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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: ComebackKid]
    #24847147 - 12/15/17 07:28 AM (3 years, 11 months ago)

Quote:

ComebackKid said:
Had this LC colonizing for almost a month now.
500ml Potato boil water with a pinch of sugar

Before the PC it was translucent but after it came out very dark brown.

The longer the myc colonizes the more it clears up. Kind of cool




Hey man, try doing less water per jar.


These are 300ml LC's. Colonized in 2 weeks. 1.200ml of LC in total and less eggs in one basket, or however the fuck that phrase is said.


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InvisibleViolet
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24847244 - 12/15/17 09:26 AM (3 years, 11 months ago)

Can second that.  My 500mL quart jar LCs siting right next to my 250mL ziploc container LCs have taught me this.  Both LCs appear to grow as much mycelium in the same time period.  Meaning liquid drawn up from the short Ziploc containers is thick with mycelium way sooner.

So, more liquid height takes longer.

But possibly not as much so with more liquid width, so it seems at least, though we probably don't have many different wider container options to see.


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InvisibleTheMadHatter420
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Re: The Biopsy Method: A different way of doing LC. [Re: Violet]
    #24847290 - 12/15/17 10:01 AM (3 years, 11 months ago)

I am rocking out some 400ml Grain water LC. I added 0.5g dextrose to each jar. I was using one LC on maybe day 10 at the latest. They colonized nicely!


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InvisibleJHOVA
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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24847364 - 12/15/17 10:42 AM (3 years, 11 months ago)

Hey josex is it possible to pc syringes with water in them? From what i have read the pressure empties out pre filled syringes.


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24847369 - 12/15/17 10:44 AM (3 years, 11 months ago)

Its in the tek.  Of course its possible... :confused:


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InvisibleJHOVA
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24847379 - 12/15/17 10:47 AM (3 years, 11 months ago)

I read cronicr saying the water ejects on multiple threads :shrug:

is there a certain orientation of the needle that prevents water leaking?


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24847387 - 12/15/17 10:50 AM (3 years, 11 months ago)

Quote:

JHOVA said:
I read cronicr saying the water ejects on multiple threads :shrug:

is there a certain orientation of the needle that prevents water leaking?




It's all in the OP brutha mang. If you don't read the whole TL;DR you are sure to miss out something important. It's the section "prepping the syringe".


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InvisibleFungus Mountain
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24847394 - 12/15/17 10:54 AM (3 years, 11 months ago)

Ok, talking about nasty looking LC. Has anyone tried making a lions mane  LC. That shit looks freaky! I'll have to snap a pic later so I can show off my LC collection. :strokebeard:


--------------------
“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
George Orwell, 1984

"If you can't explain it simply, you don't understand it well enough."
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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24847397 - 12/15/17 10:57 AM (3 years, 11 months ago)

Quote:

JHOVA said:
I read cronicr saying the water ejects on multiple threads :shrug:




Stand the syringe up. Water wont eject.  Physics bro:thumbup:


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InvisibleJHOVA
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24847421 - 12/15/17 11:16 AM (3 years, 11 months ago)

Ok ill test it out. Upon further reading i see pussy fart mentioning rapid depressurization can cause the water to eject out of a filled syringe. So dont be a dumbass and operate the PC  per usual.


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24847644 - 12/15/17 01:24 PM (3 years, 11 months ago)

damn, my windows phone takes better pics of LCs than the 500$$ nikon. i need to learn to use that bitch some day:crazy:

my first time doing penis evny #6


nice little white fuzz ball bottom left. xtra lite malt:thumbup: 1g per 400ml.

this one is nasty grain water. unflitered. just scooped out after the grain boil and PCed. 1/4cup grain water to 250ml water


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InvisibleComebackKid
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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24847808 - 12/15/17 02:40 PM (3 years, 11 months ago)

Quote:

Fungus Mountain said:
Ok, talking about nasty looking LC. Has anyone tried making a lions mane  LC. That shit looks freaky! I'll have to snap a pic later so I can show off my LC collection. :strokebeard:



:popcorn:


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InvisibleFungus Mountain
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Re: The Biopsy Method: A different way of doing LC. [Re: ComebackKid]
    #24848140 - 12/15/17 05:18 PM (3 years, 11 months ago)

Here is the Hericium. Has a pretty unique appearance.


Random LC's that are on deck. 


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Offlinetombosley8
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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24848780 - 12/15/17 11:25 PM (3 years, 11 months ago)

unfiltered with metal lids rubber side down?

i'VE BEEN THINKING of doing some lcs(well I already did one) in qt jars with a good amount of airspace above the liquid and unfiltered unmodded plastic lids.

Should that be enough air for proper growth of say 400ml of lc or will it stall or not grow properly due to lack of ge?


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InvisibleFungus Mountain
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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8]
    #24848820 - 12/15/17 11:52 PM (3 years, 11 months ago)

Unfiltered, flipped metal lids, gasket up. Hard to see, but the bottom one is 600ml and fully colonized, no stalling has been observed. On the contrary, it's starting to get thick and continuing to grow.


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“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
George Orwell, 1984

"If you can't explain it simply, you don't understand it well enough."
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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24849693 - 12/16/17 01:27 PM (3 years, 11 months ago)

Hell yea FM, you rockin it! :rockon:

Tom, I think the problem with plastic lids is that they're bulky and can be awkward to handle if we compare it with metal lids, where you only need to do a quick fluid motion to inoculate.
You can do it as long as you feel comfortable using plastic lids and you're confident you can distinguish a bacterial LC. I personally wouldn't.
CBK said the other day that he had had problems in the past with platic lids for LC's.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24849721 - 12/16/17 01:44 PM (3 years, 11 months ago)

Plastic lids suck :thumbdown:


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24849734 - 12/16/17 01:54 PM (3 years, 11 months ago)

my texas goliad totes are looking bad ass:thumbup: the recovery and coco colonization are insanely quick too.

:wellhellothere:

ill post some pics when its more than brown coco with white myc. i think we all know what that looks like by now:tongue:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24849743 - 12/16/17 01:57 PM (3 years, 11 months ago)

Problem is, there's nothing more unforgiving than LC's, things you can get away when noccing grains can bite you in the ass with LC's. The more measures we take to ensure sterility the better.

Quote:

mushboy said:
my texas goliad totes are looking bad ass:thumbup: the recovery and coco colonization are insanely quick too.

:wellhellothere:

ill post some pics when its more than brown coco with white myc. i think we all know what that looks like by now:tongue:



:popcorn:


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24849757 - 12/16/17 02:05 PM (3 years, 11 months ago)

i thought a one piece plastic lid would be better for LCs, like this salad dressing jar..
yes its karo, yes i tossed it:facepalm3:

..took me a few inoculation sessions but i cant fucking stand the 1 piece plastic lid.
its surprisingly awkward compared to the metal lid thats so easy to pop n drop.(inoculate)


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