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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835745 - 12/09/17 01:34 PM (3 years, 10 months ago)

Anyway malt look darker because it's full of all sorts of good shit.

Proteins from the malt, free amino nitrogen, enzymes, sugars, etc..

The only difference between LC and agar is the agar. LC is known as broth by all the rest of science.


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835753 - 12/09/17 01:39 PM (3 years, 10 months ago)

Josex Tek says 1g for 500ml, mushboy confirmed this, and somegirl corrected me as well and said she uses 1g for 500ml :shrug:


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835754 - 12/09/17 01:40 PM (3 years, 10 months ago)

Quote:

bodhisatta said:
Whos doing 0.2% we usually use 2% so 20grams into 1L





It's in op 1-2gs per 500ml.

Bod pcing hdpe for 15 minutes yay or nay


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24835766 - 12/09/17 01:43 PM (3 years, 10 months ago)

I use more :shrug:


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835771 - 12/09/17 01:46 PM (3 years, 10 months ago)

If 1 gram works why waste the rest?


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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24835780 - 12/09/17 01:49 PM (3 years, 10 months ago)

Likely more mycelial mass, and intracellular energy reserves. Thats just a hunch based on what I know works for yeast propagation.

Yeast likes around 8% for growth aerobically to get the best growth potential.


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835788 - 12/09/17 01:52 PM (3 years, 10 months ago)

Do you notice any increase in colonizations with a stronger broth?


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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24835893 - 12/09/17 02:36 PM (3 years, 10 months ago)

Shit I had been using0.5-1g/500ml so my mycelium mass doesn't get to damn thick. The couple times I used 2g it was crazy thick and a bitch to get into the needle.


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835897 - 12/09/17 02:38 PM (3 years, 10 months ago)

Quote:

bodhisatta said:
I use more :shrug:





i made a broth with 5gram of poorly powderized oats. colonized the same as the others. jars are colonizing as we speak:thumbup:
i also did 2g with unfiltered brf and it was so thick and nasty i couldnt even see the myc let alone take a decent pic.

mateah-just make some broths with different ratios and report your findings:shrug: so far i like ~1g per ~400-500ml.

Quote:

TheMadHatter420 said:
crazy thick and a bitch to get into the needle.




follow the tek and pour that thick nasty shit. colonizes hella quick.
youll see a white glob ontop of the grains after pouring. 2days later is myc explosion:eek:


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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24835900 - 12/09/17 02:38 PM (3 years, 10 months ago)

16gauge?


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835905 - 12/09/17 02:40 PM (3 years, 10 months ago)

No mine was so thick I had issues with the LC dispenser needles clogging with mycelium.


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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24835907 - 12/09/17 02:41 PM (3 years, 10 months ago)

Glass shards in your jar and a shake really help


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24835954 - 12/09/17 03:04 PM (3 years, 10 months ago)

I followed the tek as written, several times with the same results. Used 1g/500ml LME, no filter, just the metal lid flipped, poke and squirt per OP. Left to sit to one week, I didn't fuck with it at all (pic is posted, no stir aids). After one week, I could see healthy colony growth with no indications of bacteria. 2nd week LC was agitated twice daily. Healthy growth, and well dispersed by day 14 and ready to go.

I'm an LC noob, so take my results with a grain of sand. Just saying that I followed it as close as I could and it works like a champ! I'd recommend that everyone give it a shot as written before adding their own flair to it. The results are inspiring.

:awesantabig:


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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24835966 - 12/09/17 03:08 PM (3 years, 10 months ago)

the cleaner your cultures the better your LC. ive noticed what i thought was 'clean enough' for grains might not be that clean after all.:facepalm3:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24836102 - 12/09/17 04:19 PM (3 years, 10 months ago)

:whathesaid:


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24836191 - 12/09/17 05:18 PM (3 years, 10 months ago)

Glass shards didn't work so well for me, but 3 glass marbles seem to shred it for me, for some reason. I guess their mass assists in a stronger swirling motion or some shit, lol.


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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24836194 - 12/09/17 05:19 PM (3 years, 10 months ago)

I broke a pint glass some pieces are marble sized at least.


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24836202 - 12/09/17 05:21 PM (3 years, 10 months ago)

Quote:

mushboy said:
the cleaner your cultures the better your LC. ive noticed what i thought was 'clean enough' for grains might not be that clean after all.:facepalm3:





Yea those will only really work for cakes, lol. I have been using agar for grains, and LC for cakes. LC is not something I need, but I make it to learn a new skill, and figure why not put it to use.


@Bod - I used a cracked half pint jar. Broke it up with a hammer and put 3 pieces in my jars. Not sure if the fractures just left the edges to dull or what, but hell marbles work. I guess it is possible my pieces were to small, about the size of a nickel.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24836729 - 12/09/17 10:36 PM (3 years, 10 months ago)

There was some discussion earlier in this thread about smaller gauge needles.  Just FYI, blunt 25GA (1.5") is too small, you can't squirt at all.  22Ga 1" sharps squirt well, I have blunt 1.5" coming from amazon.

The 25Ga will be great for spores though, you can only get one drop out at a time, slowly.


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Re: The Biopsy Method: A different way of doing LC. [Re: somegirl]
    #24838012 - 12/10/17 06:15 PM (3 years, 9 months ago)

Lc 13 days in. Been swirling for about 4 days


Lc poured over rye five days ago shaken 1.5 days ago. Looks weird I've never messed with LCS any thoughts?



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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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