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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24829624 - 12/06/17 12:20 PM (3 years, 10 months ago)

I don't understand inoculation via ship :shrug: doesn't make a lick of sense.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

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:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24829673 - 12/06/17 12:40 PM (3 years, 10 months ago)

Josex would you highlight the visual characteristics of LC vs bacteria for the uninitiated such as myself using the aforementioned LC.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24829711 - 12/06/17 12:56 PM (3 years, 10 months ago)

I did what I could here. I think a beginner could easily mistake small partciles floating in the LME broth for bacteria. If you can see the particles when you shine a flashlight through the jar, no matter how tiny these are, you can be sure that's just sediment floating around. A bacterial LME LC will get murky and lose the initial color the liquid started with.

I started doing this when I was a total LC noob a year ago, wouldn't know then how to tell a bacterial LC apart. I just tried to always use clean cultures and it never gave me trouble.

If you suspect a LC can be bacterial but don't feel like trashing it, you could just dip a flamed inoc loop in it and test it on agar.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24829874 - 12/06/17 02:30 PM (3 years, 10 months ago)

Quote:

Josex said:
you could just dip a flamed inoc loop in it and test it on agar.




thats a really good idea:thumbup:

i just dumped an LC i knew was bad. had a slimy film on the liquids surface.. shoulda taken a picture:facepalm3:
but ill been spawning a batch of texas goliad in a few days. poured on the 28th. shaken on the 2nd. almost 100%

used 200ml poured into 9quarts


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24829889 - 12/06/17 02:39 PM (3 years, 10 months ago)

A film? that's weird. Was it a GW LC or lme?

I have 6 lc's going right now at various stages of colonization :awesomenod:
I poured one yesterday into 13 jars. It's a PE clone from an agar pin but I don't know how the hell is gonna perform.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24829899 - 12/06/17 02:46 PM (3 years, 10 months ago)

brf. the slim looked like floating trash and the broth smelled like bad cottage cheese.
i put 3 pin clones to lc last night:thumbup: 1 ape, 1 pe6, 1 texas. in a few days ill be doing a pan cambo LC.:awesanta:

all poked. all poured. no SHiPs!! i followed the tek:smirk:


...no blunt needles either.


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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24829977 - 12/06/17 03:16 PM (3 years, 10 months ago)

I poured mine yesterday.

I need a fh lol


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24829984 - 12/06/17 03:20 PM (3 years, 10 months ago)

Check out stareatclouds last vid on G2G. You can pour LC's in a similar way, but pouring liquid is far easier than doing G2G. Hell I remember the first few times I poured, all nervous and shaky, and all went great, no need for a fh. :shrug:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24830003 - 12/06/17 03:29 PM (3 years, 10 months ago)

I saw that actually looks pretty good :thumbup:

I don't need a fh I just want one so i can do more at jars at a time. It was a bit awkward since my lc was a wide mouth and my jars are regular but it did make me more careful.

The first time I did a G2G I did it in a bathroom ousted out. Went well surprisingly. The master was spore to grain flamed sterilized wiped with 91%iso and shoved thru polyfil lmao. The good times.


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24830047 - 12/06/17 03:58 PM (3 years, 10 months ago)



--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
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Invisiblevan hattonFacebookDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24830060 - 12/06/17 04:06 PM (3 years, 10 months ago)

I was going to use my one liter media bottle I have for this :rockon:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24830063 - 12/06/17 04:09 PM (3 years, 10 months ago)

Yeah media bottles should be awesome for this. A ghetto version would be a small water bottle with a screw cap, no need to filter it, cap screwed all the way during colonization. Lc's don't seem to care about GE.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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InvisibleGerms
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24830090 - 12/06/17 04:24 PM (3 years, 10 months ago)

$48 for a cap... Looks like I’m in the wrong industry.

Pouring is easier than g2g IME as well. Both are easy. Liquids are just easier


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24830212 - 12/06/17 05:40 PM (3 years, 10 months ago)

Quote:

Josex said:
You don't have cube myc there. Inoculating through a ship again? Using that bacterial culture you said you used to inoculate? Follow the tek dude, you aren't doing this tek.




If this is not mycelium then I have the same contam in 4 LCs, and I opened the lid, I use ships mostly for LI. I didnt plan on using this LC on anything it was just to test how it worked so I dont have anything to test it on besides a dish, thats what Ill do to confirm what is growing. I didnt even clean the jars before making this LC they were from the store straight to LC jars and there is a lot of dust in there, but next time when I make LC that im gonna use ill make the LC more clear and with a clean culture.


--------------------
Water Tub


Edited by Mateja (12/06/17 05:41 PM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Mateja]
    #24830230 - 12/06/17 05:48 PM (3 years, 10 months ago)

I don't know what that can be tbh, never had growth like that before in an LC.
I did all the testing for you man, you don't need to do half assed attempts to know how this works.
You seem very meticulous in everything you do, apply it here man. I really want you to succeed, not for the sake of the tek but for yourself.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24830246 - 12/06/17 05:55 PM (3 years, 10 months ago)

first of all i think the LCs were a success, i had nothing else besides brown sugar, i added only 3g to 100ml water and didnt even expect it to work. Second i dont see my self as very meticulous, just curious. If I was making this LC to inoculate to get clean spawn I would have cleaned the jars first before use, and I wouldnt have used 3g of brown sugar. No need to get hung up on my "half asses attempts" im just bumping your thread to report back that i got something in my jars and that im glad it worked, when i test it on a dish ill report back again and tell you what it was. peace


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Water Tub


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Re: The Biopsy Method: A different way of doing LC. [Re: Mateja]
    #24830268 - 12/06/17 06:06 PM (3 years, 10 months ago)

:hug:
I don't care bout the thread being bumped tbh, all I care about is bringing all the projects I have to fruition, mashrooooms!

You can get LME online, is cheap and a bottle can last you like forever, it could even outlive you.
Brown sugar...that could be the reason for the shitty growth, dunno.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24830281 - 12/06/17 06:14 PM (3 years, 10 months ago)

If you don't have LME, why not make some grain flour LC? Surely you have some grains laying around, right? Just grind up some grains and throw a pinch in each LC jar. Its easy. :thumbup:


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Re: The Biopsy Method: A different way of doing LC. [Re: herrenvolk]
    #24830298 - 12/06/17 06:23 PM (3 years, 10 months ago)



300ml of thick LGT LC, gonna prep some grains tomorrow. This time I used BRF and 2 fuckin grams, to remember the old times. There was sediment like you wouldn't believe but the myc has grabbed it all already, what a hungry pig.
:africaface:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24830301 - 12/06/17 06:28 PM (3 years, 10 months ago)

i used 5grams bro and grew tons of shrooms with that LC:derp:

i ordered some lme powder so my noob ass can identify/observe the myc growth. i have a kickass nikon d5600 so i can take some sexy LC closeups:thumbup:


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