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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24803926 - 11/24/17 01:26 PM (3 years, 10 months ago)

But we're not trying to kill bacteria in the grains, those get killed easily. We want to destroy the endospores that are very tough.


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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24803932 - 11/24/17 01:28 PM (3 years, 10 months ago)

Well also that bad grain prep can also make improper sterilization a possibility. Since we are using steam to sterilize we need x amount of water present within the grain to achieve proper sterilization within the jar.

I only think that because I don't see how even with a 3 hour cycle the steam can penatrate the middle of a quart jar from outside forces. Plus have to work through a filter. :shrug:.

I agree that sterile technique needs to be on point if you expect clean spawn. But if our grains are better prepped we are lower our rate of improper sterilization. Which I personally enjoy consistent results. Ive also had bad experience with poorly prepped spawn it was a nightmare.

I switched my prep and instantly saw better results.


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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24803937 - 11/24/17 01:29 PM (3 years, 10 months ago)

Quote:

bodhisatta said:
Even if oats were bad it should have absolutely nothing to do with mold contamination



:cookiemonster:

But sometimes it does.  You are in farm country.  Your oats better be kickass. I got mine at a roadside TSC sale on my way to the beach:shrug:

Ive gone through hundreds of lbs of wbs and nothing has ever been like those bags of oats.

Ill just blame the oats.  Its easier than debating a billion things and it gets me back into my seeds and now my ego is protected.

Yes..  Thats right.
:smuglook:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24803942 - 11/24/17 01:32 PM (3 years, 10 months ago)

:lolsy:


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LC's are easy
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Clean yo shit
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InvisiblebodhisattaMDiscordReddit
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24803945 - 11/24/17 01:33 PM (3 years, 10 months ago)

Any of the agricultural spores that they might treat grain with like BT, Bacillus thuringiensis. Should be completely killed off. I think a common misconception is that there's endospores inside of the grains. Ive seen no evidence of that.


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InvisibleGerms
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24803948 - 11/24/17 01:33 PM (3 years, 10 months ago)

Quote:

mushboy said:
Quote:

bodhisatta said:
Even if oats were bad it should have absolutely nothing to do with mold contamination



:cookiemonster:

But sometimes it does.  You are in farm country.  Your oats better be kickass. I got mine at a roadside TSC sale on my way to the beach:shrug:

Ive gone through hundreds of lbs of wbs and nothing has ever been like those bags of oats.

Ill just blame the oats.  Its easier than debating a billion things and it gets me back into my seeds and now my ego is protected.

Yes..  Thats right.
:smuglook:



I feel somewhat along the same page. Tried three different brands and a few different preps but the spawn is never as clean as wbs with the same culture, and I know the oats can’t be that shitty cuz this is fuckin Texas.

For stones tho oats have been seeming to do astronomically better than wbs


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Re: The Biopsy Method: A different way of doing LC. [Re: Germs]
    #24803980 - 11/24/17 01:55 PM (3 years, 10 months ago)

Indeed.  I learned oats so i wouldnt be picking or eating wbs off my stone run.

But all those were awesome.  I lost 1 out of 48 stone jars and it was from sloppy g2g and the trays ive spawned just sit for months growing more stones in the coco/sand i spawned them too.

:shrug:

Fukkit mang.  I got 100lb of wbs to prep tonight. :awedance:


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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24807126 - 11/26/17 10:39 AM (3 years, 10 months ago)

4 days in and I've got a fuzzy GH dot in my LME broth. I have a few extra plates, so I'm also going to try a couple of transfers using this method as well.

:fuckinawesome:


--------------------
“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24807132 - 11/26/17 10:46 AM (3 years, 10 months ago)

:highfive:

Good dude! The good thing about LME is that if it doesn't get cloudy with bacteria and myc does not just stay at the very bottom of the jar like a slob you can bet your shit's clean. Mold would be more difficult to see (I guess, never happened to me) but you'd need to try hard to get mold with the poke, unless it was in the culture already.

Congrats dude!


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
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InvisibleFungus Mountain
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24807196 - 11/26/17 11:34 AM (3 years, 10 months ago)

Thanks man! I've tried a few other LC methods, but this one is far and away the simplest.

Keep up the good work!


--------------------
“Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
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"If you can't explain it simply, you don't understand it well enough."
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Re: The Biopsy Method: A different way of doing LC. [Re: Fungus Mountain]
    #24807966 - 11/26/17 05:51 PM (3 years, 10 months ago)

I'm making 4 LC's with a bacterial MS plate. I bumped the armholes a few times during this transfer and it showes. The transfer came from a clean plate so stabbing away at this culture should be safe right guys? I have a lot of clean plates that I can use instead but to honour this method I would like to make four clean LC's from an otherwise unusable plate for inoculation :smile: I will inoculate both grain and flour with these liquid cultures so we'll see soon enough what will become! Cheers



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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24807971 - 11/26/17 05:54 PM (3 years, 10 months ago)

Quote:

Josex said:
myc does not just stay at the very bottom of the jar like a slob




so if its settled its no good?


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24808030 - 11/26/17 06:30 PM (3 years, 10 months ago)

Quote:

mushboy said:
Quote:

Josex said:
myc does not just stay at the very bottom of the jar like a slob




so if its settled its no good?




Yeah that's a bad sign. I just threw an GW LC down the toillet like that, cos my impatient ass can't wait to have an obvious clean culture with a PE clone I have. So don't rush it kids, get a fucking nice clean culture and then expand the shit out of it via LC.
The smell coming from the LC I just dumped was horrible. Mind you clean LC's don't smell pretty either but bacterial LC's smell disgusting.

If you're gonna make GW LC with sediment that's pretty much the clue that tells you something's fucky, myc settling I mean.
Also, there's a good reason I don't like to add anything to agitate the LC when I make GW with sediment. I want to leave the colony alone doing its thing the first few days. If it's healthy, there should be several puffy balls of myc at the bottom that can grow pretty big with time. If the LC is contaminated, these balls of myc won't grow big, but rather they'll start slowly spreading throughout the bottom and you can tell the myc is weak.
When the puffs look healthy I start swirling the jars daily and the myc will try to colonize the whole LC very actively. It's tough to explain I hope you get what I mean.

Again, LME LC's are very noob friendly in that respect, it is very easy to tell when one is contaminated with bacteria. So if you are not sure you can tell when a GW LC is contaminated I'd rather go the LME route.


--------------------
LC's are easy
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Edited by Josex (11/26/17 07:15 PM)


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24808090 - 11/26/17 07:06 PM (3 years, 10 months ago)

:takingnotes:

i poured some last night and i DID NOT shake them. gonna let em chill until i see some good recovery.

the first lc i ever made was with spores and i remember that smell when i went to 'test it' like hot molding cheese.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24808114 - 11/26/17 07:17 PM (3 years, 10 months ago)

What have we got there? APE?


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LC's are easy
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Clean yo shit
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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24808120 - 11/26/17 07:22 PM (3 years, 10 months ago)

:hamletmonkey:

this culture

i did the poke a few days before this pic.

and i did the biopsy sample poke on this one. violating the tek.:blush:


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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24808166 - 11/26/17 07:40 PM (3 years, 10 months ago)

poured right at midnight.

already seeing recovery.

i cased the plate i poked:naughty:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24808169 - 11/26/17 07:40 PM (3 years, 10 months ago)

That first plate mang, fuckin rights :rockon: Reminds me a lot of my fake APE, growth's very similar.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24808173 - 11/26/17 07:41 PM (3 years, 10 months ago)

getting some pins


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24808179 - 11/26/17 07:43 PM (3 years, 10 months ago)

First time I see a petri cased with verm, that's a good one :cool:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
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