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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24799979 - 11/22/17 03:05 PM (3 years, 10 months ago)

:creepylurker:


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LC's are easy
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InvisiblemushboyMDiscord
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24799995 - 11/22/17 03:16 PM (3 years, 10 months ago)

right now its more like...:disappoint:

and doing open air on a mono is a bitch. thats a fuckload of misting bros:thumbdown:


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Offlinetombosley8
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
    #24801743 - 11/23/17 10:09 AM (3 years, 10 months ago)

Will do some magic with this one
ms lgt 5th xfer


Edited by tombosley8 (11/23/17 10:10 AM)


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8]
    #24801756 - 11/23/17 10:20 AM (3 years, 10 months ago)

What are we seeing? It looks like a quart jar with water, an agar culture you poked and a wedge missing from it.

What's that magic?:eek:

Edit: Ha! I see whatcha doing. Gonna blender that after having poked it and taken a wedge, nothing goes to waste. :hehehe:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


Edited by Josex (11/23/17 10:38 AM)


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Offlinetombosley8
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24801825 - 11/23/17 11:13 AM (3 years, 10 months ago)

yep exactly. Thanks for playing. :cultureclash:

and also going my old route with it from Li to lc broth except using your grain lc broth instead of mea...

Definitely one of my better/best looking lgt plates so I put it to a lot of good use "magic"


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Edited by tombosley8 (11/23/17 11:16 AM)


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8]
    #24801831 - 11/23/17 11:17 AM (3 years, 10 months ago)

Yep, that plate looks wonderful, very uniform nice growth. Me likes :awesomenod:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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Offlinetombosley8
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24801839 - 11/23/17 11:22 AM (3 years, 10 months ago)

Thanks I needed that!


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InvisibleTheMadHatter420
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24801843 - 11/23/17 11:23 AM (3 years, 10 months ago)

I got some jars rocking fast as hell from LC made this way. Now I did pour the LC and ended up with like 20-30ml per jar but some will be fully colonized in right around a week, tomorrow.


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Offlinetombosley8
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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24801849 - 11/23/17 11:27 AM (3 years, 10 months ago)

Not seeing any growth from 4 days ago on a couple biopsy Lc inoculations. It's kinda hard to see through it, I thought I saw a small dot of floating myc but then I lost it really quick. Think I'm just overly anxious.

oh yeah haven't seen any growth on the agar plates either that were nocced with the biopsy 4 days ago.

Sure something will pop up soon/
oh yeah the wheat is much clearer than oat lc


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Edited by tombosley8 (11/23/17 11:28 AM)


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24801855 - 11/23/17 11:29 AM (3 years, 10 months ago)

I've also accidentally poured way too much in some occasions. Nutting ever went wrong with those jars. The reason I say 'pour with caution' in the OP is mainly because you could pour less liquid per jar and do a shit ton more jars which equals more spawn fuck yea.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24801863 - 11/23/17 11:35 AM (3 years, 10 months ago)

Hey Tom, I understand your concern man. If this is the first time you do this you're gonna be all anxious and needing to see some myc asap.

LME lets you see everything, as you know. When you gain trust in the method and have done it plenty times, you won't care at all about going full-on nasty mode.:crazy2:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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InvisibleTheMadHatter420
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24801866 - 11/23/17 11:38 AM (3 years, 10 months ago)

I used grain soak water diluted to 25% and tossed in just a bit of dextrose. I PC'd with 3 marbles in each jar, for 1 hour. I shook 2-3 times a day, marbles help a lot. I saw growth rather fast, and it expanded fast as fuck from fragmenting the mycelium.


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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
    #24802102 - 11/23/17 01:40 PM (3 years, 10 months ago)

Maybe I missed in op but josex you should add those pics of lc after 7 days you have in journal land.


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24802122 - 11/23/17 01:51 PM (3 years, 10 months ago)

Those pics are old as hell dude haha
And I don't want to clutter up the op with pics. Currently I mostly do nasty GW LC and never take pics of them.
I had to make a single LME LC on purpose to take a pic for the tek.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24802134 - 11/23/17 02:03 PM (3 years, 10 months ago)

:rockon: for sure


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24802238 - 11/23/17 02:57 PM (3 years, 10 months ago)

All josex LC'd and shaken after inoculation mindful to not wet the filters. Recovery visible in 1 day bit 8-10 days to full colonization with 10cc LC inoculation.



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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24802242 - 11/23/17 02:59 PM (3 years, 10 months ago)

:thumbup:


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InvisibleJosex
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24802342 - 11/23/17 03:59 PM (3 years, 10 months ago)

Quote:

bodhisatta said:
All josex LC'd and shaken after inoculation mindful to not wet the filters. Recovery visible in 1 day bit 8-10 days to full colonization with 10cc LC inoculation.





:cookiemonster:

:dogpipe:


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
LAGM '21

PHENO HUNTERS


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24802885 - 11/23/17 10:25 PM (3 years, 10 months ago)

:sad:

mono got the mean green. i officially suck and i officially banish oats from my house to the depths hell.
i cant help but think its more than just coincidental that with everything i poured, only the oats tammed out after spawning.

either way, i should of poured all my grains in one session but meh. that what happens when you say 'fukkit' too many times:nono:

first time ive seen green pre flush 2 in like ...uh ..:shrug:


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Re: The Biopsy Method: A different way of doing LC. [Re: senseit]
    #24802938 - 11/23/17 10:57 PM (3 years, 10 months ago)

:kenthumbup:


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