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InvisibleViolet
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Registered: 12/06/11
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24741348 - 10/27/17 01:41 PM (6 years, 3 months ago)

Cool stuff josex.

Blunt tips help.


When I started I did the original lc biopsy tek where the biopsy is of a fruit. 3 tried, 3 succeeded, proved to be a single step contraction of biopsy cloning and creating a powerful inoculant as per this tek.  I would agree it's a noob method though as it's preferable to hold on to isolated cultures from a source prior to such expansion, and both cloning and LCs should be learned on their own first instead of mushing them into one.


These days I'm also back into LCs, thanks to grainwater.  You may find it interesting that Ziploc twist n loc containers can apparently host LCs without modifications whatsoever. I've done a number of LCs to maturity in them that have exploded into gorgeous mycelium that cleared up thick grainwater. Somehow these containers 'breathe' without the spread of contaminants.
They're then simply poured open lid in a session as you do.  Or since I use sterile airflow I'm able to draw much of the liquid up into syringes first thing for storage and later use.


I may try a syringe biopsy for starting my next LCs from master culture. I have been growing out an agar ziploc container then squirt & scrape to create some LI for an LC and a couple ryegrass masters.  Figured more mycelium would expand to LC faster but it may not end up mattering much.

But I'm particularly interested in the agar poke method here for taking multi-spore culture samples.  Seems like an easy, instant, precise method to isolate different new cultures from germination plates into separate grows like in my grain petri culture hunt tek.  I like using different tools like tweezers and screwdrivers but I think the blunt needle and water plunger might be the perfect tool for that first step of many containers.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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InvisibleViolet
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24745167 - 10/29/17 10:04 AM (6 years, 2 months ago)

Quote:

Josex said:
Also, I would not do this method poking an invitro agar pin as Cron said, I'd transfer the pin to a blank first. My confidence in pp5 containers with snap-on lids has shaken quite a bit after my last mold disaster a few months back. They're far from perfect and shit can def get in them if you keep them for long till they pin.



I hope you took note of the detail I offered in my last post, about the screw-top lids even working unmodified for LC.

With the exception of fewer fitting in the cooker, I can hardly understand why a ton more people aren't using these ziploc containers for cultivation. They're like a dream come true for every utility I've found so far for Cubensis cultivation and most utilities growing in general. Essentially I find them only poorer for constant spawn production.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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InvisibleViolet
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24745384 - 10/29/17 11:51 AM (6 years, 2 months ago)

Huh, I found that blunt needles worked better.  The needles are called hypodermic which means adapted for use in or administered by injection beneath the skin, they're designed NOT to cut a sample. Of course you're still touching them to your culture regardless, we throw medical use needles away after their use because they are contaminated by the contact.  But when I do a clone scrape using a needle (still often to agar) I am combining a scrape with the puncture motion, otherwise I found it just pushes through which you do not want for a fruit body clone.

The blunt tip made stab motions more successful for me in taking a cylinder, although sometimes for fruit tissue I would still end up scraping.



As for the containers.  I guess in part thanks to my clean success rate and clever little culturing steps I end up using much less agar and fewer dishes of any sort as a whole.  It's a transfer or storage medium for me, and I have gotten to running a very tight ship.  If I trimmed down to a collection of just 30 containers to turn around, I could still fairly easily operate all my gourmet culture handling and all my exotic culture hunting.

shhh....


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy] * 1
    #24745626 - 10/29/17 01:44 PM (6 years, 2 months ago)

I know, the fruit clone was brought up as an aside, but I was still using it for comparison for this as well, as to why I was surprised you commented that you figured blunt tips may not work as well.  I might not have understood perfectly if you weren't staying away from blunt needles.

I can't see any downsides to using such a tiny sample as even a 31G would take for expansion to a grow-out.

Also, I wasn't shushing you about what you're doing, that was as if I was showing you a part of a little personal secret.
What you're doing is fine, great even.  I used to boom out lots more agar plates as well, but since then I've decided just to trim it down to the smaller focus, only telling you about what I do and why I don't mind the size.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Edited by Violet (10/29/17 01:54 PM)


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InvisibleViolet
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24774396 - 11/10/17 06:43 PM (6 years, 2 months ago)

That's perfectly reassuring. I'm gonna do syrige agar pokes for my next round of grain petris culture hunting.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: ComebackKid]
    #24778516 - 11/12/17 06:53 PM (6 years, 2 months ago)

I love the unmodified upside-down metal lid when using jars. Have a little overflow of no-pour agar in some now.  Can vouch it works.  But then again you also remember my comment here earlier about doing LC in totally unmodified ziploc containers where they work perfectly even with the lid on correctly. They're very safe. I've shaken them around like making a cocktail, and tested them clean weeks later on all grows.  Squeezing them is the only true danger, air CAN exchange if you try to make it.

I'm considering getting out of the practice of modifying glass jar lids for spawn and seeing how it goes just doing those upside down too.


You guys' LCs are making me think mine might grow out faster if I diluted my rye/wheat/ryegrass boil water.  After all, the nutrition isn't affixed in place like it is agar.  Are people still finding any differences with LC concentrations?


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24778825 - 11/12/17 09:31 PM (6 years, 2 months ago)

Quote:

Josex said:
We need someone like Ferather to conduct a weird ass shit experiment about this and then hire someone to translate what he says.



:rofl:

About the recipes, I have seen that when diluted my grain water doesn't appear to grow much less mycelium or much slower, at least.

Maybe the difference is what I consider being a grown-out LC.  I like them to occupy a good chunk of the liquid when settled before I really use them. Of course they can be used earlier, they still have myc.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24787619 - 11/16/17 05:25 PM (6 years, 2 months ago)

Josex, do you shake your jars after LC inoc?


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: Psilosopherr]
    #24791358 - 11/18/17 10:52 AM (6 years, 2 months ago)

Quote:

Psilosopherr said:
Sorry but whats the TL;DR for the OP?

Is it: Poke myceliated agar with syringe, use this to knock an LC/other plate?




Cross-contam on purpose lol


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24811610 - 11/28/17 12:02 PM (6 years, 1 month ago)

Those look so sharp. Almost makes me wanna order malt.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24847244 - 12/15/17 07:26 AM (6 years, 1 month ago)

Can second that.  My 500mL quart jar LCs siting right next to my 250mL ziploc container LCs have taught me this.  Both LCs appear to grow as much mycelium in the same time period.  Meaning liquid drawn up from the short Ziploc containers is thick with mycelium way sooner.

So, more liquid height takes longer.

But possibly not as much so with more liquid width, so it seems at least, though we probably don't have many different wider container options to see.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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