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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 6
    #24740301 - 10/26/17 11:14 PM (6 years, 3 months ago)

Quote:

hamloaf said:
Heck ya, Josex.  The biopsy LC method is an old technique.




I knew of the noob method where you stab a fruit and squirt the tissue into lc...  But not josex' method.

I am currently using his method.  Should have fruits within a week! Thanks josex for the sacred knowledge:stoned:

Super easy and awesome :thaaannks:


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Re: The Biopsy Method: A different way of doing LC. [Re: catnip40] * 5
    #24741106 - 10/27/17 11:25 AM (6 years, 3 months ago)

i cloned this bitch on the left.(normal methods. scalpel and 3 or 4 transfers.)


and tried the biopsy method and used it two ways:



WAY NUMBER THE FIRST
i just did the poke and shot the sample onto a plate. it basically grew out like a MS germ plate would.. only its a clone.

ive done this with a few other MS cultures. its really cool because you can have multiple
areas of growth on the plate from the same tiny ass sample. really makes identification of growth easier/quicker.(more pics later)

WAY PART SECOND
the way it was outlined. sample into grain water broth. i fucked up and used 5grams of oat powder per 500ml. ooops.

mega cloudy and sediment like you wouldnt believe:shrug:

i didnt upload pics because it was impossible to see the growth but it cleared up considerably as it colonized.


colonization took about 15days. i poured it in 3!!! three separate sessions.. all poured. no contamination
breakin josex' rule because im gangsta like that:cool:



(LC beats the wedge by weeks)

these colonized rather fast. i used little LC for each jar. the bag got maybe a shot glass worth at most.
the bag is about 90% colonized. each jar took about 5-10days to colonize. since i poured in different sessions
i waited for all the quarts(8) to colonize before spawning. i spawned a few shoeboxes and 2 20qt minis.
the bag will be a mono. i lost one jar, the last one i poured. i banged the SAB roof when pouring and it failed its shake.

the pouring an poking are about as basic as it gets. and pouring LC into a bag with a SAB is WAY EASIER than using a wedge.

ive failed with bags like crazy in my SAB. my fucking hands shake and i hate modding lids. for me? pouring LC is fucking awesome.

:congrats:


Edited by mushboy (10/27/17 12:03 PM)


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Re: The Biopsy Method: A different way of doing LC. [Re: cronicr] * 3
    #24741610 - 10/27/17 03:36 PM (6 years, 3 months ago)

noob method because it involves shooting dirty fruit tissue into karo water.

and this gem..

Quote:

Quickly wipe your needle with iso (flame if desired, I don't bother - we did just sterilize it after all!)




:facepalm3:

although it was 11yrs ago. back then i was a noob too. now i use josex method and i am the master.
:sagetrip:


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Re: The Biopsy Method: A different way of doing LC. [Re: ASliceofPI] * 2
    #24745163 - 10/29/17 10:03 AM (6 years, 2 months ago)

i used a flat headed needle so i had to be careful not to take a 'core sample' as josex mentioned.

you want to have a few invisible myc fragments stick to the heated needle and nothing else.
you are not taking a 'agar sample' but a 'culture' sample.:thumbup:

back to you josex:thaaannks:


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24745461 - 10/29/17 12:24 PM (6 years, 2 months ago)

i go through lots of agar myself. id need hundreds of those ziplocks but can only pc  few at a time? :obamafrown:

heres the 'core sample' floating around.

in the upper center left you can see a blurry little fuzz. if you look hard enough you can see the denser agar.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24746157 - 10/29/17 06:27 PM (6 years, 2 months ago)

:awegroove::popcorn:

ill post pics of my soon to be fruiting shoeboxes tomorrow:cool:


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Re: The Biopsy Method: A different way of doing LC. [Re: cronicr] * 2
    #24747874 - 10/30/17 02:50 PM (6 years, 2 months ago)

3quarts to 3 quarts in a 20qt mini


random shoebox


poured 6qt bag of oats. my first bag to not tam out.


pouring LCs into bags kicks ass:rockon:


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 2
    #24749949 - 10/31/17 01:11 PM (6 years, 2 months ago)

ive been using quart jars with my usual tyvek mp tape filter.

500ml of liquid in a quart still leaves plenty of headroom to stir up the broth w/out getting the filter wet.
and its super easy to pour. but i like the idea of the really small spout to pour from. less exposure=less chance of ickies.

FIRST LC KNOTS.

cant see any but ya know.. they dont show up with my shitty nokia fone.

yah dude. i got 3 more quarts to spawn tonight.. and that bag should be done by the weekend. mono town:borfase:

..and ive done about 3 rounds of this. lots of jars. lots of broth. no contams:thumbup:

now pimpin is easy :snoop:


Edited by mushboy (10/31/17 01:16 PM)


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24756540 - 11/03/17 09:36 AM (6 years, 2 months ago)

once these knots/pins grow a but ill post some more pics.


lucy/aa+ pins never show up too well on my camera. just looks like white blurs:tongue:

id also like to note..

due to my shaky fucking hands, MOST if not all of my contams happen during inoculation.
either from a bad culture drop, or i bang something.. usually 1 jar out of 10 gets an
out break of green on the surface of the grains. but with pouring?? not a single jar
was visibly contaminated. although 1 jars recovery took 4days...:cool: meh. point is?

this method is awesome for anyone who has a hard time with being clean(inoculation wise). cause im sloppy as fuck sometimes.


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24756615 - 11/03/17 10:18 AM (6 years, 2 months ago)

haha. ya dude. the 100% test. 4days and its usually in the garbage. but i spawned it anyway:shrug:

:awegroove::popcorn:


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24759107 - 11/04/17 11:47 AM (6 years, 2 months ago)

this clone is takin its sweet ass time pinning.



im never spawning while tripping again:facepalm:

and i spawned these the other day:derfase:


not happy with this clone but fukkit. its the other aspects that make this grow awesome:awesomenod:


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24763810 - 11/06/17 12:39 PM (6 years, 2 months ago)

made some ape broth with the poke:naughty:


using the blunt tipped needle is more of 'core' sample taking. both ways work. i just happen to have spare blunt tips:shrug:


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24763899 - 11/06/17 01:19 PM (6 years, 2 months ago)

60mm dishes. perfect size:thumbup:

Quote:

That's some wide ass needle you got there



:smuglook: thats what they tell me

maybe im not letting the needle cool long enough?so the core sticks inside? in fact, i barely cool. i stab shit while hot.


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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24766900 - 11/07/17 03:54 PM (6 years, 2 months ago)

no. i chop the base off to about an inch stump. then i pull the base apart like a bag of chips.. if that makes sense..

exposing making 2 halves. i then take the flamed blade and gently scrap out a sample.. put to agar. ... eat the discarded  specimen:stoned:


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Re: The Biopsy Method: A different way of doing LC. [Re: tombosley8]
    #24768792 - 11/08/17 10:02 AM (6 years, 2 months ago)

even though ive had clean clones on the first dish, id be way to scared to shot tissue into LC.

put to agar and then poke with the needle would be the way id go.  ...wait. i did go that route!


scraped out tissue from the guy on the left.

agar'ed


poked, LCed, poured, colonized, spawned


i should of cleaned up the original culture. but fuck it. it worked too easily to care. and the tiny specs
of myc from the poke slimmed down the genetics even more so maybe you dont need to use a super nice culture.


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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24768818 - 11/08/17 10:11 AM (6 years, 2 months ago)

Id just drop the pin zgzr


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24768906 - 11/08/17 10:30 AM (6 years, 2 months ago)



:cool:


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24768945 - 11/08/17 10:42 AM (6 years, 2 months ago)

:derfase:

tissue is the inoculate. lc/li is a broth to grow/suspend said tissue

thats like asking if pancakes are better than maple syrup


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Re: The Biopsy Method: A different way of doing LC. [Re: JHOVA]
    #24768988 - 11/08/17 10:54 AM (6 years, 2 months ago)

Quote:

Jonhova said:
Is that faster or about the same as using LC/LI to inoculate a 1/2 pint brf cake?




:shrug:


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Re: The Biopsy Method: A different way of doing LC. [Re: insanemike]
    #24769155 - 11/08/17 12:23 PM (6 years, 2 months ago)

being practical isnt hostile. either way, its is definitely worthy of its own thread:thumbup:

***spelling errors:uhoh:


Edited by mushboy (11/08/17 12:34 PM)


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