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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: The Biopsy Method: A different way of doing LC. [Re: van hatton]
    #24740755 - 10/27/17 08:21 AM (6 years, 3 months ago)

Nice write up brutha


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: The Biopsy Method: A different way of doing LC. [Re: FooMan] * 2
    #24741597 - 10/27/17 03:31 PM (6 years, 3 months ago)

You can also let a plate pin and do creamcorns style:cheers:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24746752 - 10/30/17 12:49 AM (6 years, 2 months ago)

Quote:

Josex said:
Wow, just read Creamcorn's tek, just wow! haha
How in the actual fuck can someone manage to pull that off without contaminating the LC? :facepalm3:

It's almost offensive to compare it with this Ham. :ohyou:

This tek is not about taking a perfectly visible core sample either but about inoculating with literally microscopic pieces of tissue from a clean agar culture to make the procedure as safe as possible, so you can perfectly get away with no testing and dicking around.

Also, I would not do this method poking an invitro agar pin as Cron said, I'd transfer the pin to a blank first. My confidence in pp5 containers with snap-on lids has shaken quite a bit after my last mold disaster a few months back. They're far from perfect and shit can def get in them if you keep them for long till they pin.



I dont actually poke the pin...i drop the whole thing in


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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