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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Buddha19er]
#24740722 - 10/27/17 07:55 AM (6 years, 3 months ago) |
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-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24752912 - 11/01/17 06:31 PM (6 years, 2 months ago) |
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Pcing some syringes and brf plates right meow
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
#24763708 - 11/06/17 11:47 AM (6 years, 2 months ago) |
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4 days I used the method with some brf plates (josex idea) nice white growth coming in.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24764373 - 11/06/17 04:24 PM (6 years, 2 months ago) |
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I'll throw some cvg in next time as well.
One culture I'm trying to clean up the second one is for fun.
Going to try this with lc when these plates grow out (if they dont comtam).
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24764932 - 11/06/17 08:10 PM (6 years, 2 months ago) |
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Different plates would be used for the lc. Should of specified.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
#24768815 - 11/08/17 10:10 AM (6 years, 2 months ago) |
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What about poking a pin on agar?
Should be sterile
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
#24768837 - 11/08/17 10:14 AM (6 years, 2 months ago) |
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I mean I definitely would as well just a thought.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
#24768977 - 11/08/17 10:51 AM (6 years, 2 months ago) |
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I know 
Bad picture look 4 transfers 2 days ago.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
#24802102 - 11/23/17 11:40 AM (6 years, 2 months ago) |
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Maybe I missed in op but josex you should add those pics of lc after 7 days you have in journal land.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24802134 - 11/23/17 12:03 PM (6 years, 2 months ago) |
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for sure
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24803804 - 11/24/17 10:37 AM (6 years, 2 months ago) |
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Every different type of grain has a different type of prep. (Duh)
I feel alot of people switch around alot. Just find the way to prep your grain and stick with it fuggit. Unless your willing to learn how to make that new grain perfect.
One thing may work for someone and not another vice versa. There are so many variable's with grain prep it makes my head spin. You really gotta tweak it yourself with what you have around you.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: TheMadHatter420]
#24803932 - 11/24/17 11:28 AM (6 years, 2 months ago) |
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Well also that bad grain prep can also make improper sterilization a possibility. Since we are using steam to sterilize we need x amount of water present within the grain to achieve proper sterilization within the jar.
I only think that because I don't see how even with a 3 hour cycle the steam can penatrate the middle of a quart jar from outside forces. Plus have to work through a filter. .
I agree that sterile technique needs to be on point if you expect clean spawn. But if our grains are better prepped we are lower our rate of improper sterilization. Which I personally enjoy consistent results. Ive also had bad experience with poorly prepped spawn it was a nightmare.
I switched my prep and instantly saw better results.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24820724 - 12/02/17 08:11 AM (6 years, 1 month ago) |
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Day 5 no growth which is not a bad thing at all. It's got that weird mod on it so at least the inside stays sterile 
Another lc finished at 19 days gunna put to agar later. This guy didn't show growth for like 6-7 days so I'm assuming I'll have the same results with the newer ones. The grain is super slow.
Edit: snapped a pic before work of the lc that's done.

-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (12/02/17 08:36 AM)
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24829624 - 12/06/17 10:20 AM (6 years, 1 month ago) |
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I don't understand inoculation via ship doesn't make a lick of sense.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
#24829977 - 12/06/17 01:16 PM (6 years, 1 month ago) |
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I poured mine yesterday.
I need a fh lol
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
|
Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
#24830003 - 12/06/17 01:29 PM (6 years, 1 month ago) |
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I saw that actually looks pretty good 
I don't need a fh I just want one so i can do more at jars at a time. It was a bit awkward since my lc was a wide mouth and my jars are regular but it did make me more careful.
The first time I did a G2G I did it in a bathroom ousted out. Went well surprisingly. The master was spore to grain flamed sterilized wiped with 91%iso and shoved thru polyfil lmao. The good times.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
#24830060 - 12/06/17 02:06 PM (6 years, 1 month ago) |
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I was going to use my one liter media bottle I have for this
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: mushboy]
#24835663 - 12/09/17 11:02 AM (6 years, 1 month ago) |
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Having some trouble find a bottle like yours that's all #5 pp.
Most of the lids seem to be #2 hdpe. While Google says it can withstand 120°c for a short time. I don't think id feel confident pcing hdpe for 15 mins.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
#24835754 - 12/09/17 11:40 AM (6 years, 1 month ago) |
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Quote:
bodhisatta said: Whos doing 0.2% we usually use 2% so 20grams into 1L
It's in op 1-2gs per 500ml.
Bod pcing hdpe for 15 minutes yay or nay
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: The Biopsy Method: A different way of doing LC. [Re: somegirl]
#24838012 - 12/10/17 04:15 PM (6 years, 1 month ago) |
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Lc 13 days in. Been swirling for about 4 days

Lc poured over rye five days ago shaken 1.5 days ago. Looks weird I've never messed with LCS any thoughts?
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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