Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Josex] * 1
    #24740236 - 10/26/17 10:39 PM (6 years, 3 months ago)

Heck ya, Josex.  The biopsy LC method is an old technique.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Violet]
    #24787654 - 11/16/17 05:34 PM (6 years, 2 months ago)

Shaking will damage the myclium.  If you want to distribute LC around the grains upon inoculation GENTLY roll the media vessel around for like a second or two.  Shaking vigorously the LC upon inoculation will cause the myce to not recover.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: bodhisatta]
    #24787684 - 11/16/17 05:40 PM (6 years, 2 months ago)

I always gently roll.  When I shake vigorously the myce doesn't recover for me.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Germs]
    #24788155 - 11/16/17 08:49 PM (6 years, 2 months ago)

Try 14 gauge.  14 has a much wider opening than 18.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Josex]
    #24788229 - 11/16/17 09:22 PM (6 years, 2 months ago)

No, I'm talking about for the aspiration of the liquid culture.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Germs]
    #24788491 - 11/17/17 01:46 AM (6 years, 2 months ago)

12 gauge needles.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Offlinehamloaf
Loaf of Fam.
Male User Gallery

Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma. Flag
Last seen: 1 year, 8 months
Trusted Cultivator
Re: The Biopsy Method: A different way of doing LC. [Re: Doc9151]
    #25147855 - 04/18/18 11:21 AM (5 years, 9 months ago)

18-20 gauge needles are actually small bore needles.  The lower the number gauge of needle, the larger the bore is, so 12-14 gauge needles are large bore needles.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Unfilter Print Post Top
Jump to top Pages: 1

North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* Storing an LC needle. NewbieS 840 3 03/12/06 02:58 AM
by skeletor
* How to draw up the "good stuff" in a large LC jar
( 1 2 all )
SWBZA 464 24 01/20/24 09:22 PM
by SWBZA
* LC extraction for inoculation>? cannabisg 848 6 12/14/06 09:38 PM
by cannabisg
* LC needle redshadow 413 2 04/03/06 08:35 PM
by trauma47645
* 18ga needle damaging SHIP on LC jar? SWBZA 67 1 01/01/24 06:51 AM
by Changed
* LC lids faceyneck 4,756 11 02/11/07 09:09 AM
by agar
* Help Finding LC Jar Tek splifner180 1,221 6 05/18/06 01:05 PM
by kilroy
* Sterile Syringe for LC JoelLoveland5 2,260 9 02/23/07 08:22 AM
by Sillicybin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
126,009 topic views. 37 members, 316 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.012 seconds on 17 queries.