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Offlinegaryguitar
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Bringing Spore Prints From the US Via Doha
    #24715800 - 10/17/17 03:07 AM (6 years, 6 months ago)

Hello all. I am on my way to the USA from South Africa. Spores are both legal in Arizona and South Africa. My problem is that I have a two hour period in transit in Doha, Qatar on my way back to South Africa. I assume that my bags will still be in the plane.

Does anyone have advice for me on whether it would be fine to have spore prints in my luggage? Or should I not risk it?

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OfflineGombaTaltos
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Re: Bringing Spore Prints From the US Via Doha [Re: garyguitar]
    #24715889 - 10/17/17 06:03 AM (6 years, 6 months ago)

Don’t fuck around in an Arab country. U probably get 25 years in prison and a beating for a spore print. Not worth the risk. Mail that shit.


--------------------

Edited by GombaTaltos (10/17/17 06:06 AM)

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InvisibleCitric
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Re: Bringing Spore Prints From the US Via Doha [Re: GombaTaltos]
    #24715936 - 10/17/17 06:42 AM (6 years, 6 months ago)

Quote:

frostmage said:
Don’t fuck around in an Arab country. U probably get 25 years in prison and a beating for a spore print. Not worth the risk. Mail that shit.




100%


--------------------
Self Healing lid tek ** Update 10.17.17 **
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!

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InvisibleCitric
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Re: Bringing Spore Prints From the US Via Doha (moved) [Re: garyguitar]
    #24715939 - 10/17/17 06:43 AM (6 years, 6 months ago)

This thread was moved from Mushroom Cultivation.

Reason:
Better place for this.

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OfflineTammy
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Re: Bringing Spore Prints From the US Via Doha (moved) [Re: Citric]
    #24716799 - 10/17/17 01:26 PM (6 years, 6 months ago)

Dont...

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InvisibleEnigma1
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Re: Bringing Spore Prints From the US Via Doha (moved) [Re: Tammy]
    #24716899 - 10/17/17 01:53 PM (6 years, 6 months ago)

Do they allow small waster bottles on the plane?

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OfflineAlan RockefellerM
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Re: Bringing Spore Prints From the US Via Doha (moved) [Re: Enigma1]
    #24717672 - 10/17/17 06:19 PM (6 years, 6 months ago)

If the bags are just sitting on the plane, you will be fine.    They are not going to take the luggage off, search it, then put it all back in.

If you are paranoid do not label them as psilocybin spores.  It is quite hard to test spores to see if they are psilocybin - they would need to germinate them, then test the culture with GCMS.  It would take weeks, minimum.

You could also mail prints in a letter.  US customs does not really open letters, they mostly check big boxes.


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Anonymous #1

Re: Bringing Spore Prints From the US Via Doha (moved) [Re: Alan Rockefeller]
    #24718341 - 10/17/17 10:26 PM (6 years, 6 months ago)

Quote:

Alan Rockefeller said:
It is quite hard to test spores to see if they are psilocybin - they would need to germinate them, then test the culture with GCMS.  It would take weeks, minimum.




What about the ability to identify spores of Psilocybe spp. using direct PCR techniques?

Thread: https://www.shroomery.org/forums/showflat.php/Number/23486807
PMID: 27021629 [for sci-hub.cc]

Actually this paper above is really about DNA analysis of subtrates inoculated with spores so maybe it is not relevant


But there are other references that make it seem like one could do taxonomic analysis from spores alone. (e.g. http://www.fgsc.net/Aspergillus/protocols/Spore%20DNA%20protocol%20Oakley.pdf)

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OfflineAlan RockefellerM
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Re: Bringing Spore Prints From the US Via Doha (moved) [Re: Anonymous #1] * 1
    #24718415 - 10/17/17 11:20 PM (6 years, 6 months ago)

Thanks for posting that paper, I had not seen it.  I responded to the thread you linked before I finished reading your post - you noticed the same thing I did, which is that they didn't identify the spores, they grew them and identified the mycelium.

I have heard that you can't sequence a spore print, and I have been meaning to try it, but haven't yet.  I'll give it a try this week.

It is typically the psilocybin and psilocin that is illegal rather than the spores, so police haven't had much incentive to identify spores.  Even in the three states where spores are illegal, I have not heard of those laws being enforced.  It is a whole lot easier to just bust the people with active mycelium growing.   

If they could identify spores they could bust some people for intent to manufacture a controlled substance for having syringes and grow supplies, but that isn't currently how police labs work.  What they do is go in, seize suspected substances, and then test it with gas chromatography.  If they don't have controlled substances, they drop the case and bust someone else that does have controlled substances.

About the protocol you posted sequencing from spores, those are conidiospores, which are asexual and have twice the DNA of the sexual spores that Psilocybe has.  Whether or not that matters, I don't know.  If you don't hear back feel free to PM me to remind me about it.  What I plan to do is scrape a thick spore print into a tube, grind with a plastic pestle to break the cell walls, extract the DNA with NaOH and run a PCR.  Would be cool if I could get good sequences out of spore prints, as some people would rather mail spore prints than actual tissue samples.


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