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OfflineDiastamasta
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Contamination of grain spawn
    #24707419 - 10/13/17 04:59 PM (3 years, 6 months ago)

Hi all,

First off this is my first post.  I would like to thank you all for your vibrant community it has helped me successfully grow using Pftek in the past.  I am now venturing into bulk grows but am coming up with contamination.  I am using Bods easy AF teks.  I have a SAB and a 23 quart PC that I am using properly for all that i can tell. 

So i took some spore prints that were originaly successfully grown using Pftek and bought from a vendor.  Made my own spore syringes using medical grade syringes and sterile water.  Followed all the steps etc.  The only thing I did not use was poly fill.  Couldn't find it at my local home depot.  Instead i used filter material for air ducts.  crammed it in the top really well.  I inoculated grain spawn in the SAB.  Everything was going well I thought during consolidation in the jars but then I saw some green bits developing.  I should also mention that the temp in the room once got up to 94 f for about 10 -20 minutes. 

Please check out the pictures and see if you can either confirm a faulty filter material or bad spore syringe or if the temp was too high allowing for contams to take over quickly.  Also not even sure if the white stuff is mycellium.  I should add that all 10 jars have similar contam patterns.

Thanks again...





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InvisiblebodhisattaM
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Re: Contamination of grain spawn [Re: Diastamasta]
    #24707470 - 10/13/17 05:29 PM (3 years, 6 months ago)

Chances are it was from making the syringe from your print. Thats why all the jars are bad


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OfflineDiastamasta
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Re: Contamination of grain spawn [Re: bodhisatta]
    #24707522 - 10/13/17 05:48 PM (3 years, 6 months ago)

Quote:

bodhisatta said:
Chances are it was from making the syringe from your print. Thats why all the jars are bad





Guess i should have followed your spore print TEK.  I have a spore print from one of the first mushrooms to bloom during my last Pftek grow.  Would that have less contams maybe?  Would testing them on agar first be agood idea?  Maybe I should just buy another MS syringe.


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InvisiblebodhisattaM
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Re: Contamination of grain spawn [Re: Diastamasta]
    #24707560 - 10/13/17 05:59 PM (3 years, 6 months ago)

Making a print and then a syringe from your print isn't going to have a 100% success rate your first times


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Re: Contamination of grain spawn [Re: bodhisatta]
    #24707624 - 10/13/17 06:38 PM (3 years, 6 months ago)

Yeah I hear you.  For now, before i jump into agar.  I think ill see if one of the jars I inoculated turns out ok.  I double checked them and it seems as if maybe one or two could be clean.  then ill G2G them.


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Re: Contamination of grain spawn [Re: Diastamasta]
    #24707626 - 10/13/17 06:40 PM (3 years, 6 months ago)

Buying a vendor syringe will be a lot more clean. Doesn't mean to give up making your own though. Youll get good at making a syringe, but agar work is highly recommend. Its easy too

Youll flub up some agar learning. Good thing its cheap. You don't learn how to have dexterity making syringes and agar plates and look like you've done it before overnight.


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OfflineDiastamasta
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Re: Contamination of grain spawn [Re: bodhisatta]
    #24707702 - 10/13/17 07:17 PM (3 years, 6 months ago)

Thanks Bod!


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OfflineCapefearjay
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Re: Contamination of grain spawn [Re: Diastamasta]
    #26925737 - 09/09/20 08:03 AM (7 months, 27 days ago)

Thanks man


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