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InvisibleStargate
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Re: Does Reishi Eat Caffeine? [Re: Alan Rockefeller]
    #24705264 - 10/12/17 05:17 PM (6 years, 6 months ago)

The tough part, is that cellulose is a very difficult organic polymer to break down. Do you know of an enzyme that breaks down cellulose easily?

I'll definitely try letting it eat to different stages.


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InvisiblebodhisattaMDiscordReddit
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Re: Does Reishi Eat Caffeine? [Re: Stargate]
    #24706584 - 10/13/17 08:31 AM (6 years, 6 months ago)

Cellulase :shrug:


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InvisibleStargate
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Re: Does Reishi Eat Caffeine? [Re: bodhisatta]
    #24707261 - 10/13/17 01:45 PM (6 years, 6 months ago)

Quote:

bodhisatta said:
Cellulase :shrug:


:facepalm3: Can't believe that didn't come to mind for me. I was just researching that a month or two ago.
Well, fungi and bacteria make cellulase. I would rather culture the bacteria or fungi that is capable of producing it. Originally, I was looking at bacteria, but was having a lot of difficulty finding a source to buy a strain I know of that can do this. Do you know of a bacteria capable of making it? Perhaps you know one that I don't.


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InvisiblebodhisattaMDiscordReddit
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Re: Does Reishi Eat Caffeine? [Re: Stargate]
    #24707273 - 10/13/17 01:51 PM (6 years, 6 months ago)

Not off the top of my head. They probably have gmo aspergillus to make cellulase. Bacteria idk


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InvisibleStargate
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Re: Does Reishi Eat Caffeine? [Re: bodhisatta]
    #24707288 - 10/13/17 01:57 PM (6 years, 6 months ago)

What do you think about pseudomonas fluorescens? It can be cultured on agar, it has a biosafety level of 1, and it should produce pretty good levels of cellulase.


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Edited by Stargate (10/13/17 01:58 PM)

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Re: Does Reishi Eat Caffeine? [Re: Stargate]
    #24707340 - 10/13/17 02:18 PM (6 years, 6 months ago)

As long as you're not elderly, a newborn, or have aids I don't see a problem growing it.


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InvisibleStargate
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Re: Does Reishi Eat Caffeine? [Re: bodhisatta]
    #24707378 - 10/13/17 02:34 PM (6 years, 6 months ago)

I feel that this experiment could be expanded at this point. A comparison of the effects on the green (non-roasted) coffee bean, from cellulase producing bacteria vs cellulase producing fungi. I'll start it with the fungi, and then when I get the bacteria in, I'll run a set of tests with bacteria. The bacteria will only cost me $20.

I can get the beans roasted, and do taste tests afterwards.

I've got a high grade coffee company that is supplying me with free coffee beans for these tests. These beans are coming from the highest mountain peak in Jamaica. They are the ones that will be roasting the beans for me as well.


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Offlinekrypto2000
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Re: Does Reishi Eat Caffeine? [Re: Stargate]
    #24707398 - 10/13/17 02:46 PM (6 years, 6 months ago)

The highest?  :awehigh:

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Mushrooms, Mycology and Psychedelics > Advanced Mycology
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