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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Vermiculite

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OfflineWeebleWobble
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Pf tek very slow growth at the end. Wet spots?
    #24704407 - 10/12/17 12:03 PM (9 days, 20 hours ago)

Four of my six jars are almost fully colonized except for some small spots. They are still filling the area, albeit very very slowly. I've taken a pic every day to compare.

I've attached a photo from Sunday and then from today, Thursday, which is day 15.

Sunday:



Thursday day 15:



What to do?  Should I wait till they fill in then count a week?  Or birth soon?


Edited by WeebleWobble (10/12/17 12:05 PM)


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OfflinecronicrM
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Re: Pf tek very slow growth. Wet spots? [Re: WeebleWobble]
    #24704410 - 10/12/17 12:04 PM (9 days, 20 hours ago)

Likely bacterial alĺ you can do is wait it out


--------------------

It doesn't matter what i think of you...all that matters is clean spawn


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OfflineWeebleWobble
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Re: Pf tek very slow growth. Wet spots? [Re: cronicr]
    #24704412 - 10/12/17 12:06 PM (9 days, 20 hours ago)

Wait for it to fill in and eat the bacteria?


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Offlinekamealh
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Re: Pf tek very slow growth. Wet spots? [Re: WeebleWobble]
    #24704856 - 10/12/17 03:40 PM (9 days, 17 hours ago)

no the mycelium / slime will fight it out and depending on how strong your mycelium is it'll either win/lose ... if it wins you'll get some slightly darker spot with puss .... okay i just grossed myself out ... I don't wanna play anymore.

I'll let you know what I did in a similar situation .... if you are certain it's slime ... then simply very slowly and carefully open up the jars one by one and extract all healthy fully colonized parts of the substrate and place it in another clean container and let them reestablish network between all the pieces ...
they will fruit and if your cake fruits.... then it's safe to eat .


--------------------
Everything I post is make belief and all the photo's I upload are just doctored photographs and are not real.
Don't believe anything I said, or will say in the future... I lie a lot.


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InvisibleCitricM
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Re: Pf tek very slow growth. Wet spots? [Re: kamealh]
    #24704865 - 10/12/17 03:44 PM (9 days, 17 hours ago)

Trace the dark spots with a marker, and give it a week.  If there is no growth, birth it, cut out the uncolonized portions, dunk, roll, double end case, and fruit it.

If it does advance and grow past your marks, leave it be untill 100%, wait a week, and proceed as normal.


--------------------
Self Healing lid tek ** Update 10.17.17 **
Fast, easy, fool proof inoculation
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Cup O' Shrooms
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Offlinekamealh
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Re: Pf tek very slow growth. Wet spots? [Re: Citric]
    #24706571 - 10/13/17 10:25 AM (8 days, 22 hours ago)

it blows my mind the ability for mycelium to detect slime at the bottom of the jar and in turn completely stop growing ....


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Don't believe anything I said, or will say in the future... I lie a lot.


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Offlinekamealh
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Re: Pf tek very slow growth at the end. Wet spots? [Re: WeebleWobble]
    #24706573 - 10/13/17 10:26 AM (8 days, 22 hours ago)

by the way is the jar warm?  as in does it feel like the jar has a fever ?
if yes then thats a contam


--------------------
Everything I post is make belief and all the photo's I upload are just doctored photographs and are not real.
Don't believe anything I said, or will say in the future... I lie a lot.


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OfflineMateah
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Re: Pf tek very slow growth at the end. Wet spots? [Re: kamealh]
    #24706620 - 10/13/17 10:52 AM (8 days, 22 hours ago)

I see four express way tunnels on top of every jar for bacteria and mold to go trough, why aren't those holes covered?

Also, when you have bacteria and mold sitting on top of your verm layer then you shouldn't even move those jars one inch before full colonization, but I bet you have picked up every one of those jars and handled it for inspection twisting and tilting the jars (if ever so slightly) but this is not a good procedure for you considering your inoculation holes are not covered.

Another thing that also has to do with the uncovered inoculation holes is that when you pull the needle out some water or LC or what ever medium you inoculated with will smear off on the sides and on top of the verm layer. So EXACTLY where the inoculation holes are for contam to go trough exactly THERE have you probably made the verm layer wet and nutritious, just think about that for a sec.

I could possibly be very anal about these BRF cakes right now but this is just for reference so you know what you can do better next time. :smile:

What did you inoculate with?


--------------------
The most important thing is to find out what is the most important thing


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OfflineWeebleWobble
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Re: Pf tek very slow growth at the end. Wet spots? [Re: Mateah]
    #24711301 - 10/15/17 10:19 AM (6 days, 22 hours ago)

Quote:

Mateah said:
I see four express way tunnels on top of every jar for bacteria and mold to go trough, why aren't those holes covered?

Also, when you have bacteria and mold sitting on top of your verm layer then you shouldn't even move those jars one inch before full colonization, but I bet you have picked up every one of those jars and handled it for inspection twisting and tilting the jars (if ever so slightly) but this is not a good procedure for you considering your inoculation holes are not covered.

Another thing that also has to do with the uncovered inoculation holes is that when you pull the needle out some water or LC or what ever medium you inoculated with will smear off on the sides and on top of the verm layer. So EXACTLY where the inoculation holes are for contam to go trough exactly THERE have you probably made the verm layer wet and nutritious, just think about that for a sec.

I could possibly be very anal about these BRF cakes right now but this is just for reference so you know what you can do better next time. :smile:

What did you inoculate with?




I've repeatedly read that the holes do not need to be and should not be covered, that the verm layer is all you need.

The bottom right jar is almost fully colonized now, maybe two more days. The center jar has filled in a little more but there's still a tiny spot. Perhaps it was uneven verm.

The other jars aren't doing as well I think. As an experiment I flipped one, propped up, which is recommended by some.

Yea, I moved the jars some. :frown:


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OfflineWeebleWobble
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Re: Pf tek very slow growth at the end. Wet spots? [Re: kamealh]
    #24711304 - 10/15/17 10:21 AM (6 days, 22 hours ago)

Quote:

kamealh said:
by the way is the jar warm?  as in does it feel like the jar has a fever ?
if yes then thats a contam




Not sure, I can check. I've tried smelling them and don't smell anything.


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OfflineLemurLemur
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Re: Pf tek very slow growth at the end. Wet spots? [Re: WeebleWobble]
    #24711344 - 10/15/17 10:55 AM (6 days, 21 hours ago)

Who tf told u that? Cover them with 3m micropore tape or make SHIPS with rtv silicone.
Your really lucky mold hasn't gotten in.


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InvisibleTheMadHatter420S
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Re: Pf tek very slow growth at the end. Wet spots? [Re: LemurLemur]
    #24711360 - 10/15/17 11:09 AM (6 days, 21 hours ago)



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OfflineWeebleWobble
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Re: Pf tek very slow growth at the end. Wet spots? [Re: LemurLemur]
    #24711367 - 10/15/17 11:12 AM (6 days, 21 hours ago)

Quote:

LemurLemur said:
Who tf told u that? Cover them with 3m micropore tape or make SHIPS with rtv silicone.
Your really lucky mold hasn't gotten in.




This popular thread says no tape.


Dont cover the lids after inoculation with anything example, Foil/tape
Give them love


https://www.shroomery.org/forums/showflat.php/Number/11585613/fpart/1


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InvisibleTheMadHatter420S
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Re: Pf tek very slow growth at the end. Wet spots? [Re: WeebleWobble]
    #24711387 - 10/15/17 11:24 AM (6 days, 21 hours ago)

Well we now know that Micropore tape works just fine. Hell we use it for our no pour agar plates.


--------------------


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All great art is made from suffering, and so are we!


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OfflinemushboyS
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Re: Pf tek very slow growth at the end. Wet spots? [Re: kamealh]
    #24711446 - 10/15/17 11:48 AM (6 days, 21 hours ago)

Quote:

WeebleWobble said:
Quote:

LemurLemur said:
Who tf told u that? Cover them with 3m micropore tape or make SHIPS with rtv silicone.
Your really lucky mold hasn't gotten in.




This popular thread says no tape.


Dont cover the lids after inoculation with anything example, Foil/tape
Give them love


https://www.shroomery.org/forums/showflat.php/Number/11585613/fpart/1




the dry verm layer is your filter. but if you are noobly and look at your shit every 10 seconds,
teh dry layer can be compromised. i would always use mp tape. even when i did pf. i always taped the holes.

mostly because i was a noob and sometimes i did weird shit.. like lots of noobs do. CYB:cheers:

and that jar has a fever shit is laugh out loud lolwut to the max


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OfflineWeebleWobble
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Re: Pf tek very slow growth at the end. Wet spots? [Re: mushboy]
    #24711527 - 10/15/17 12:27 PM (6 days, 20 hours ago)

I'll use tape next batch.


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Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Vermiculite

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