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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: catnip40] 1
#24747202 - 10/30/17 09:16 AM (6 years, 5 months ago) |
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Aerobic organisms usually don't enjoy high co2 environment even if they can tolerate it. Interesting enough cubes don't have a high co2 part in their life cycle anyway. If paul never published those books i bet cultivation would look different today.
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mushboy
modboy
Registered: 04/24/05
Posts: 32,782
Loc: where?
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: bodhisatta]
#24747229 - 10/30/17 09:24 AM (6 years, 5 months ago) |
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ill keep fruiting at spawning for sure.
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pixelpopper
Crap Artist
Registered: 09/20/13
Posts: 4,022
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Pastywhyte]
#24747257 - 10/30/17 09:36 AM (6 years, 5 months ago) |
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Quote:
But this was a pretty small sample. I want to run this again with more clones and a broader spectrum of tubs just to clear out the noise.
Will be looking forward to seeing a followup and how it compares.
Edited by pixelpopper (10/30/17 09:37 AM)
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Ferather
Mycological
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: pixelpopper]
#24748011 - 10/30/17 04:11 PM (6 years, 5 months ago) |
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I actually agree with you bod.
If you put an agar sample or a wood peg into a centrifuge vial, it will stall almost strait away. I'm yet to see mycelium do anything when fully cut off from O2, not without help.
Interestingly though, low O2 does cause mycelium to "push out". However more decay is better than a simple push out.
Even high humidity can lower O2 levels.
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Xclozure
Stoned as shiiitakes
Registered: 09/09/12
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: catnip40]
#24829815 - 12/06/17 12:04 PM (6 years, 4 months ago) |
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Has anyone copied this and tested it?
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,844
Loc: Canada
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Xclozure] 1
#24829867 - 12/06/17 12:28 PM (6 years, 4 months ago) |
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I was gonna run it again once i get a new clone.
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Xclozure
Stoned as shiiitakes
Registered: 09/09/12
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Pastywhyte]
#24829886 - 12/06/17 12:37 PM (6 years, 4 months ago) |
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I was thinkin about introducing fruiting this shopebox of tamps i layered coir/verm on 5 days. Do you think its a good idea pasty?
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Ferather
Mycological
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Pastywhyte]
#24829898 - 12/06/17 12:45 PM (6 years, 4 months ago) |
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My opinion is, we should stop using CO2 references, it even gave me a totally wrong impression. Can we start using high, medium, low, O2 instead, as a reference it's more accurate.
Not bashing, it's just we are talking about fruiting an aerobic organism. Mycelium respond to high or low O2 value over anything else.
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Low O2 reduces decay via oxidization, and produces low nutrient like settings. These settings cause mycelium to "push out", even when fruiting.
We all know fruit bodies will "push out" to high O2. If high O2 is reached the cap gets bigger.
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This also suggests that an oxidization process occurs in the cap. Perhaps the cap also acts like a lung, and light catcher.
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Ferather
Mycological
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Ferather]
#24830036 - 12/06/17 01:54 PM (6 years, 4 months ago) |
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Here is a visible example of mycelium going from low O2, to medium O2, as supporting evidence of Pastywhyte's findings. This is WL-Tek, using budgie seed spawn (8%, dry-to-dry), using the grow-up method (bottom-to-surface).
As the mycelium expands, near the surface it detects "higher O2", and "changes" growth pattern.
It was breathing O2 (has correct GE), it fruited only at the surface.
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shroomNdoom
Mycophilia
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Pastywhyte]
#24930105 - 01/20/18 07:50 PM (6 years, 2 months ago) |
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Been doing it the old way for years. Read the entire thread and it makes sense. I have an aggressive Chilean strain on rye that broke through the coir/verm casing and threw up aerial hyphae in 24 hours. It seems almost a shame to force it into an extended colonization period. So after 24 hours I switched from duct tape to micropore and gave it some light. Here we go!
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bobwastaken
under construction
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Posts: 1,973
Loc: SA
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: shroomNdoom]
#25059635 - 03/13/18 02:24 AM (6 years, 1 month ago) |
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Cool experiment. Would be interesting to see how casing/taping at spawning compares to applying the casing after full colonization.
Edited by bobwastaken (03/13/18 02:25 AM)
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JHOVA
Post whore
Registered: 02/17/17
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: bobwastaken]
#25060215 - 03/13/18 10:02 AM (6 years, 1 month ago) |
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Why would you case and keep tape on? Thats just a top layer.
If you are gonna case early just put it in fruiting conditions asap and shave off 1-2 weeks.
See [here] for more info
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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bobwastaken
under construction
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: JHOVA]
#25060514 - 03/13/18 12:58 PM (6 years, 1 month ago) |
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By adding the casing after spawning you slow down colonization of the casing. You can remove the tape when its ready.
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JHOVA
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: bobwastaken]
#25060546 - 03/13/18 01:17 PM (6 years, 1 month ago) |
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Isnt that the antithesis of this thread
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Failboat
Fuck Up
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: JHOVA]
#25060570 - 03/13/18 01:25 PM (6 years, 1 month ago) |
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Ok so if I understand correctly; in the shoebox tek if dub tubbed the growth should speed up and more quickly begin pinning due to higher o2 levels?
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bobwastaken
under construction
Registered: 06/17/10
Posts: 1,973
Loc: SA
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Failboat]
#25061252 - 03/13/18 06:23 PM (6 years, 1 month ago) |
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It's a tweak to the experiment. Will be testing this with a Psilocybe Alutacea clone when it's done.
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ghaleon
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: bobwastaken]
#25082003 - 03/22/18 09:46 AM (6 years, 28 days ago) |
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Doing the same thing here. 1 quart spawn (WBS) to 2 quarts of bulk substrate (CVG) with jiffy mix as casing. 8 days since spawning and the casing is partially colonized and there's alot of hyphael (sp) knots. Guess I'm supposed to just leave it alone and let it do its thing?
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Pastywhyte
Say hello to my little friend
Registered: 09/15/12
Posts: 37,844
Loc: Canada
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: ghaleon] 1
#25083404 - 03/22/18 08:05 PM (6 years, 27 days ago) |
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Yep just check to make sure it doesn't dry out and mist it if it's dry. Otherwise leave it the hell alone and you will be rewarded.
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Ferather
Mycological
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: Pastywhyte]
#25084293 - 03/23/18 07:47 AM (6 years, 27 days ago) |
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Are you still going to investigate this topic or are you happy with results? Growing oysters, which we all know, definitely needs more O2 to fruit correctly. Oxidization requires oxygen to work, however since Cubensis lacks all-most laccases it's requirements for O2 should be much lower.
Lignicolous mycelium will be utilizing laccase which requires O2 to function correctly, and decay phenols.
What I wonder is, do the active materials, which oxidize, benefit from more or less O2.
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GroovyGeorge
Camper Dude
Registered: 02/17/20
Posts: 2
Loc: USA
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Re: Case and fruit at spawning vs colonization in elevated CO2 [Re: bodhisatta]
#26492327 - 02/18/20 07:04 PM (4 years, 1 month ago) |
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Sorry I know this is mad late and you might know by know by now but I was just in another thread (I forget which) but the consensus is that it really depends on the pheno for how much CO2 is best/when.
-------------------- I'm just trying to grow some cubes,man
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