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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Pasty Agar Tek [Re: waxbrain]
#24667522 - 09/28/17 03:46 PM (6 years, 4 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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waxbrain
That Fun Guy



Registered: 05/13/17
Posts: 678
Last seen: 2 years, 10 months
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Re: Pasty Agar Tek [Re: cronicr]
#24667529 - 09/28/17 03:48 PM (6 years, 4 months ago) |
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Quote:
cronicr said:

Good deal cronicr, appreciate it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,467
Loc: where?
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Re: Pasty Agar Tek [Re: waxbrain]
#24667530 - 09/28/17 03:48 PM (6 years, 4 months ago) |
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Quote:
waxbrain said:
Wish I knew more and didn't have to ask every time there's an issue.
wwellll.. the search feature is awesome.. or.. do what i did. pick a grower you can follow/relate too, and thread stalk them. like, read every fucking post they ever made.
and when you read teks, dont just read the first post. read every page. even if the thread has billion pages..
shroomery+phone+toilet=no more constipation and more shroom knowledge.
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waxbrain
That Fun Guy



Registered: 05/13/17
Posts: 678
Last seen: 2 years, 10 months
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Re: Pasty Agar Tek [Re: mushboy]
#24667597 - 09/28/17 04:05 PM (6 years, 4 months ago) |
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Haha mushboy im on the forums constantly, I just figured I'd ask the questions that could help others or something really specific that I can't find. I've even read tens on the SE lol..
I don't attempt to annoy anyone and do use the search engine for a lot of things, it just makes me happy to see an answer to a question I had asked and not just UTFSE. It's nice. especially when you have zero people to talk to about a hobby that is dominating my life right now so I can get my mind off of other shit.
I didn't mean to blow up earlier either, I apologize. My values are very important to me and that came out as aggression in my opinion.
I really do hope everyone has a good night and weekend. Love those who have made this possible and extensively answered my questions (or had me figure it out myself then feel like a dumbass )
This has made a huge huge huge impact on my life and I literally cannot wait to take it to the next level with edibles and medicinal as well. If anyone has either of the spores/cultures (for the portabello of course!) of the mush in my signature please send me a PM I'm very grateful for everything.
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Variegated
Pressure cooker operator



Registered: 01/15/17
Posts: 820
Last seen: 2 years, 4 months
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Re: Pasty Agar Tek [Re: waxbrain]
#24667694 - 09/28/17 04:30 PM (6 years, 4 months ago) |
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I mix mine and let them sit out for hours depending on how busy i am. They firm up like that then re liquify in the pc. Those look like mine did first time. Only advice i can give is let them cool 100% in the pc so they set up nice and level. I have moved warm plates and had them get kind of thin on one side or the other when they firmed up from me tilting them. Didnt hurt anything but im a litle ocd and didnt like it lol
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 hours, 46 minutes
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to each their own, but you aren't eating the food coloring anyway.
Quote:
waxbrain said: Wish I knew more and didn't have to ask every time there's an issue. Thank you though anyone that can help me.
read every link in the first link in my sig and you will have no more questions. and I mean every link that is linked in every link. then if you have questions then you know where to ask
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Blah. I didn't let the temperature come up slowly and wound up with a bunch of soaked paper towels. I think I'll just replace them with fresh ones and hope the wet filter doesn't cause any issues.
Started doing pour agar and forgot my roots! Long live Pasty Plates!
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 hours, 46 minutes
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micropore is waterproof, you're fine!
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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I don't use micropore tape. I'm more concerned about the paper towel staying wet for so long, sitting on top of the surgical tape I use. I just feel like resting moisture that isn't evaporating quickly can't be too great, heh. I'm sure it'll be fine enough, though.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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yeah the pp towel should dry pretty fast with the foil off mine were always damp coming out of the pc but I would let them sit for a couple days before use to reduce condensation and by that point the pp towel was always dry.
I forgot the reason for the pp towel does it help with condensation? or keeping th filter dry?
because it seems to do the opposite for me.
the micropore dried up faster for me without the paper towel.
If those filters are left in open air with a wet filter for too long they will contam.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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I think the initial reasoning was for protection, but people stopped using them down the line. I keep mine on because I write on them and you can use them probably 15-20 times before there's no space to write on. I usually keep mine in a shoebox tote for some protection, but I'm leaving these out to dry.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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also I think pasty leaves the foil on until inoculation with no troubles. I have seen wet filters kept in near airtight conditions where they don't have a chance to dry, nearly all fail after a week or so with mold colonies. That's why I leave them out in the open or in a tote with an imperfect seal if I don't use them right away.
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mezzy jeb
Skywalker


Registered: 07/28/12
Posts: 291
Last seen: 2 years, 5 months
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Hi so I know it's common practice to wipe everything in an SAB down with alcohol before doing any work, and it makes sense: even from the PC to the SAB they can pick up some dirty stuff.
My question is - do people wipe down the tops with alcohol too? That gets the tape wet, and though alcohol dries quickly it makes me think if it's even worth it, especially since if you use the tinfoil and paper towel it should be pretty dang sterile on top there.
Thanks for any input
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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you can wipe around the rim and the container but the pp towel or filter shouldn't be wiped.
Also If using fresh out of pc straight to the sab I rarely wipe them at all as they were exposed to very little air and thus contams.
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Steevo
Just a cog in the death wheel



Registered: 03/02/17
Posts: 2,702
Loc: Here and There
Last seen: 1 year, 10 months
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I put holes in the sides of my containers so I don't need to cover the tops at all
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 24 days
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Re: Pasty Agar Tek [Re: Steevo]
#24686497 - 10/05/17 05:21 PM (6 years, 4 months ago) |
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good idea.
I use half pint wide mouth jars and unmodded plastic lids so no filter and no foil.
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Edited by tombosley8 (10/05/17 05:22 PM)
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waxbrain
That Fun Guy



Registered: 05/13/17
Posts: 678
Last seen: 2 years, 10 months
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I've had my pasty plates in the fridge inside my PC after cooling for a couple days now. Will leaving the towel under the foil for an extended period of time cause contains. I have company and might not be able to get it for a couple more days. Did thirty plates so I hope they're fine lol.
Do they need to be room temp to inoculate? And one more question - do the contents inside the plates need to be solid? When I had them out before PCing I could slowly move the contents like a slurrry - what I've seen seems to be that the recipe should be solid for PDA..?
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Re: Pasty Agar Tek [Re: waxbrain]
#24689189 - 10/06/17 06:22 PM (6 years, 4 months ago) |
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Quote:
waxbrain said: I've had my pasty plates in the fridge inside my PC after cooling for a couple days now.
You don't need to do that at all. Best thing you can do is take the plates out of the pc as soon as the pressure drops and when they're still hot leave them on a table (open air no problem) to solidify, that way the surface of the agar will be level once it cools down. You can store the blank plates in a tote and they'll stay good a really long time. I've had uninoculated plates in a tote for more than a month and used them without a hitch.
Quote:
waxbrain said: Will leaving the towel under the foil for an extended period of time cause contains.
No.
Quote:
waxbrain said: Do they need to be room temp to inoculate?
No.
Quote:
waxbrain said: And one more question - do the contents inside the plates need to be solid? When I had them out before PCing I could slowly move the contents like a slurrry - what I've seen seems to be that the recipe should be solid for PDA..?
After pouring your plates you should always wait until they solidify before introducing them in the pc, just to make sure they're good to go. It sounds like you either didn't wait enough for the agar to solidify before loading the plates in the pc or maybe you just simply made an useless bacth that won't solidify. They should all be solid, PDA, LME, DFA or any other recipe. It's the agar that solidifies, the ingredients have nothing to do. Not all agar-agar are the same so you should adjust how much you use.
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Variegated
Pressure cooker operator



Registered: 01/15/17
Posts: 820
Last seen: 2 years, 4 months
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Re: Pasty Agar Tek [Re: Josex]
#24689332 - 10/06/17 07:08 PM (6 years, 4 months ago) |
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I added no extra nutes to my last plates but still not seeing rhizo type growth. Maybe the second or 3rd transfer with no extra nutes?
Edited by Variegated (10/06/17 07:24 PM)
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BreakTheSOB
Stranger



Registered: 07/09/17
Posts: 162
Last seen: 6 years, 3 months
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Try 1 gram agar and .8 gram lme per 50 ml to start. Maybe .7 if no rhizo. Mine are usually in .9 range.
-------------------- "A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle
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