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northstar777 said:
Okay So Ive been lurking here for awhile I tried to get spooled up doing this back in November. love you guys by the way. At some point I was planning on doing a proper write up about my experience, and questions. (I didnt want to clutter up this place with my noobness) but I need some feed back now.

grow bag with treasure coast MS I am sure it bacterial done with one lb grain bag spawned with hpoo substrate. out of 3 bags this is the only one that consolidated. 

why is that cluster looking sick and how does the substrate look is it too/wet too/dry? I dont know how to read it.






also this is my next project




how does the tub look?

thanks

and yeah i am already doing agar work to get clean spawn.

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Tookitooki said:
Clarification on spawn bags and SAB work. 

.2 micron filter, not pre-sealed.  PC in a converted aa941 sterilizer. 

Is it advisable to seal the bags, the way they vacuum down, or should one unwrinkle the opening and seal while pulling tight? Im trying to avoid pin holes along the seal.

If running a sterilizer that pulls a hard vacuum, can one get away with a sealed bag? If so what are the precautions to take?

Thank you

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northstar777 said:
sorry to come back but opened this tub today and smelled strongly of vinegar.



here is the shoe box I am working on

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cronicr said:
Fuzzy feet is also a simple genetic thing and can pop up in even outdoor grows.
Size is mainly dependant on 3 things...food water and genetics so with heavy pinsets you will get smaller fruits

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So its reasonable to assume, with multiple strains and test bins that my problem stems more from proper nourishment and hydration to support a heavy pinset growing to large sizes? I should have at least 1 bin with large fruits if genetics was the culprit yes? Nourishment can't get any better than 2 quarts of oats soaked in coffee and gypsum and put straight to coir and verm? Do I need to start using Hpoo?

So hydration of the sub? I notice after cultivating the first flush that the sub is as dry as can be. even though the surface is still moist from misting. If the sub hasn't receded from the sides of the bin how do I get more water in there?

Edit: Im doing shoeboxes with a solid 3-3.5 inch sub depth.

Edit #2: Here is a picture to help


Seriously thumping my head why I can't get 5-6 height out of these guys.

Thank you very much for responding Cronicr

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verum subsequentis said:
if there is trich on an agar plate...

  1. does it always eventually sporalate?

  2. can it be identified without a microscope prior to sporalation?

I've grown ridiculous amounts of green lately and i CAN not understand where it's coming from.

if you think it's in my agar you can view my agar in the dick link in my signature.

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verum subsequentis said:

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verum subsequentis said:
if there is trich on an agar plate...

  1. does it always eventually sporulate?

  2. can it be identified without a microscope prior to sporulation?

I've grown ridiculous amounts of green lately and i CAN not understand where it's coming from.

if you think it's in my agar you can view my agar in the dick link in my signature.

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also, how much should i expect to spend on a microscope capable of determining whether or not my plates have trich? what sort of microscope should i be looking at?

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I’m no TC but FWIW:

A biological specimen microscope or soil scope would be ideal

Here’s the one I have. http://www.microscopenet.com/40x400x-trinocular-compound-siedentopf-microscope-soil-microscopy-p-9807.html?gclid=EAIaIQobChMIjYKs77vL2QIVBLXACh3AjQpfEAQYCiABEgIhZvD_BwE

I plan on upgrading the 40x to 60x or maybe 100x oil immersion for looking at spores at some point but Its low on the list of priorities rn.



When you get it, please help contribute to my thread.
https://www.shroomery.org/forums/showflat.php/Number/24873961

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verum subsequentis said:

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verum subsequentis said:
if there is trich on an agar plate...

  1. does it always eventually sporalate?

  2. can it be identified without a microscope prior to sporalation?

I've grown ridiculous amounts of green lately and i CAN not understand where it's coming from.

if you think it's in my agar you can view my agar in the dick link in my signature.

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also, how much should i expect to spend on a microscope capable of determining whether or not my plates have trich? what sort of microscope should i be looking at?

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Yeah trich spores are relatively large compared to say penicillium. Which is probably best seen at least at 100x. You can probably get away with 60x for most molds. However, when it comes to agar take a pic and post it. Trich is much different from it's commonly mistaken counterparts aspergillus or penicillium (who are also much more common on agar) because they have a much larger concentration of spores in the air. They all sporulate on an agar dish, either upon agitation/stress, or Fae. Keep in mind all of these can quite easily be identified and isolated away from your myc on agar WITHOUT a microscope. 

Here's some examples.

Trich:

Sporulated from stress


Exposed to more airflow.

Penicillium sporulating after it got to about a nickel size:


Sporulated from having enough GE (polyfil filter on the first and I'm not sure about the 2nd)

Aspergillus:

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Kizzle said:
Here's one with hidden Aspergillus.


This is a clone of one of the agar pins.

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Sporulated from stress

Sporulated from getting large enough/enough GE.

If your transfers are clean, chances are your shit is clean. Green mold on agar is much harder to hide.