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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Pastywhyte] * 1
    #24679346 - 10/02/17 08:16 PM (6 years, 3 months ago)

:deathrush::fuckthepolice:
And no
:derail::melt:

:bonghit:


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: potnug] * 1
    #24679384 - 10/02/17 08:28 PM (6 years, 3 months ago)

4-6 per brick give or take.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: TheMadHatter420]
    #24681468 - 10/03/17 04:03 PM (6 years, 3 months ago)

Quote:

TheMadHatter420 said:
Sorry I don't have a pic to show it, BUT I have had cultures with a really nice leading edge. I take a couple transfers to new plates only to have them look well translucent and almost non existent. I have always tossed them and snagged another transfer from the previous plate. It just looks well weak and sick as it doesn't grow nice white mycelium like one would expect. Any ideas what is happening??



Did you change media or stay the same?
Sometimes not changing media can cause problems. Sometimes a new media can too. And they otherwise might work just fine other times.
I would switch the media up and see if it helps as a first try


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Qayyn Eb-Ur]
    #24701510 - 10/11/17 07:46 AM (6 years, 3 months ago)

You cant grow a.muscaria anyway.

Spores should germinate on mea just fine try root if you want


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: cronicr]
    #24702954 - 10/11/17 05:27 PM (6 years, 3 months ago)

Hitting 170f for 10m is a do over situation for pasteurization


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: pezhvak]
    #24705044 - 10/12/17 03:20 PM (6 years, 3 months ago)

Its all white all the way around then yes. From the picture if its like that on all sides it is 100%


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: stareatclouds]
    #24706924 - 10/13/17 11:22 AM (6 years, 3 months ago)

You do close off GE on slants all the way after they colonize. Theb store them.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: mushroom_tip]
    #24707779 - 10/13/17 06:00 PM (6 years, 3 months ago)

I wouldn't make it much more than a few hours in advance. highly nutritious media that's not sterilized will mold up in a day or two


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: pezhvak]
    #24709067 - 10/14/17 10:20 AM (6 years, 3 months ago)

Quote:

pezhvak said:
I'm about to drill some holes for my monotube, some of the pictures have it in substrate level while others are 5" above the substrate, which one is right and why?



Because it doesn't really matter that much


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: ShroomyToons]
    #24709310 - 10/14/17 11:46 AM (6 years, 3 months ago)

Quote:

pezhvak said:
awesome! okay, I did my monotube and everything is done. I covered the holes with microtape for colonizing, is it okay to use it in fruiting phase too? or it has to be polyfill?



https://www.shroomery.org/forums/showflat.php/Number/23651523/fpart/all/vc/1

Quote:

ShroomyToons said:
I did use the search function but couldn't find an answer, so thought I'd ask here.

I have a good problem of having too many colonized grains for the room and number of tubs I have. How long can I store colonized cubensis grain in the fridge and still have good success with spawning?



Long enough, more than months. Probably would still try to use sooner than later


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Germs] * 1
    #24709606 - 10/14/17 01:32 PM (6 years, 3 months ago)

Quote:

Germs said:
Can bacteria or other contaminants land on agar and not germinate because of there only being one or two certain unfavorable nutrients, but then when expanded to grains or substrate then the contaminant shows up? Or will anything that lands on the agar start to grow?



A lot of contaminations might not grow on agar and will on something else. Or vice versa may do well on agar and poorly on grain.

Total plate count agar used in QA will grow a lot of aerobic organisms.

For what its worth PDA and MEA and YPD all grow a lot of things well.

Things that spoil beer are anaerobic and very specilaised to tolerate the high abv and hop bitter acids. Theres very special media incubated anaerobically to grow these microorganisms. But not all media grows all spoilage bugs. So many breweries use 2 or 3 different beer agar recipes to plate finished beer on so that hopefully if there is any bugs it will grow on one of the recipes.

Theres a big brewery in town that had an infection they were completely blind to since the two agar mediums they used to test against didn't grow the bug that was in their beer. I got a yeast sample from them and it tested bad. Low and behold it only grew on BMB agar which they didn't use. They ended up having a million dollar product recall because the spoiler eventually over carbonated bottles. It would have cost them so much less if they stopped the problem earlier than spending money on cardboard boxes, six pack holders, bottles, crowns, labels, labor, refrigeration, shipping, and then having to reship them back, and destroy them.

Rather than dumping it out before even filtering it.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: van hatton]
    #24709661 - 10/14/17 01:52 PM (6 years, 3 months ago)

Quote:

van hatton said:
So would grain water agar be best for the purpose of finding these things.

Since it's the grain were growing on anyways wouldn't anything that would grow on grain be the same for the agar? And vice versa.



Likely. Thats the idea behind diy beer agar.
Theres other media they make. Such as universal beer agar UBA it has things like tomato extract to help encourage colonies of beer spoliling lactobacillus to form since they like to grow in beer but not on agar a lot of times


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: van hatton]
    #24709695 - 10/14/17 02:05 PM (6 years, 3 months ago)

Nope just make PCR cheaper and cheaper. Agar for QA will be old news in a few years once even mom and pop places can afford something that gives results in the same day vs waiting 7 for incubation

Agar will still have use but no one will be trying to find spoilers with it in industry

Even universal beer agar isn't universal they're always trying to make a better recipe that grows every spoiler known


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Munchauzen]
    #24709732 - 10/14/17 02:21 PM (6 years, 3 months ago)

Quote:

Munchauzen said:
Quote:

Germs said:
anything that lands on the agar start to grow



nothing hides on agar



That's just not true. tuberculosis wont grow on any of our agars, but theoretically could get on one. Same goes with anaerobic bacteria, if not all of them die they still may be transferred around on aerobic plates. Which is a problem growing yeast on ypd then using it for beer in which a unseen anaerobic organism can later find itself in an appropriate home


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Germs]
    #24709740 - 10/14/17 02:24 PM (6 years, 3 months ago)

Streptococcus would not grow well on our plates if at all. But you could spit on a plate and it could get there. Theyre usually facultative anaerobes so they'll likely form no colony. And would thus hide on typical media

Hospital labs work would be a lot cheaper and easier if they only had to use MEA


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: F. 3]
    #24712762 - 10/15/17 07:37 PM (6 years, 3 months ago)

Hopefully does well. 28c is very hot try 18-24 20-22is a good range


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: KidMyco4296]
    #24714480 - 10/16/17 02:55 PM (6 years, 3 months ago)

Contamination. If you have a microscope make a slide and you'll be able to get a better idea


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: KidMyco4296]
    #24714505 - 10/16/17 03:02 PM (6 years, 3 months ago)

Transfer clean mycelium to a new dish and toss that one. Or just toss that one


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: KidMyco4296]
    #24714535 - 10/16/17 03:15 PM (6 years, 3 months ago)

Looks blurry and out of focus


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: KidMyco4296]
    #24714560 - 10/16/17 03:22 PM (6 years, 3 months ago)

Thats bacteria


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