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InvisiblehamloafS
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Re: The Official Woodlovers Thread [Re: moricz] * 1
    #25132767 - 04/12/18 11:31 AM (11 months, 4 days ago)

Always with the informative posts.  Nice one, moricz.  :thumbup:


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InvisibleLizardWizard
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Re: The Official Woodlovers Thread [Re: hamloaf] * 3
    #25132803 - 04/12/18 11:51 AM (11 months, 4 days ago)

Serbica patch colonizing nicely, had to move it though...






That last pics tells me it won't be lost in this place though, and I informed the new caretaker of the place of the nature of the spot, so if he would want to, he could add fresh wood chips every year...


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Invisiblebacillus
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Re: The Official Woodlovers Thread [Re: LizardWizard] * 4
    #25133431 - 04/12/18 04:52 PM (11 months, 3 days ago)

A short outdoor story of mine



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OnlineAdas
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Re: The Official Woodlovers Thread [Re: bacillus]
    #25133486 - 04/12/18 05:25 PM (11 months, 3 days ago)

Like a boss.


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InvisibleNothingsChangedS
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Re: The Official Woodlovers Thread [Re: Adas]
    #25134711 - 04/13/18 04:15 AM (11 months, 3 days ago)

Nice job. You guys are killing it. Thanks for you contributions to life.


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InvisibleRogerTheRetard
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Re: The Official Woodlovers Thread [Re: NothingsChanged]
    #25136345 - 04/13/18 07:17 PM (11 months, 2 days ago)

:whathesaid:
Astonishing would be an understatement.


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Re: The Official Woodlovers Thread [Re: RogerTheRetard] * 2
    #25136928 - 04/13/18 10:53 PM (11 months, 2 days ago)

Some P cyanescens germinated on no pours. Had some bacterial spots but I let em run and the myc covered everything. Added the cardboard later to test the cultures vigor. This was week or two ago, they are mostly white with myc now, time to transfer to something else.



Ovoid myc in a tub outside


First ovoid pins in my lilac pot!



I have some azzi, alleni and cy grain jars from spores ready I plan on expanding this week, I'll post some of those too. Good work folks this thread is dope!


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Re: The Official Woodlovers Thread [Re: Topcorn]
    #25138835 - 04/14/18 05:51 PM (11 months, 1 day ago)

How do you guys prep woodchips and egg cartons? Do you just soak overnight, or do you PC or something else?


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Invisibleopenmind
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Re: The Official Woodlovers Thread [Re: Ghost]
    #25138970 - 04/14/18 06:39 PM (11 months, 1 day ago)

Quote:

Ghost said:
How do you guys prep woodchips and egg cartons? Do you just soak overnight, or do you PC or something else?






I don't know what's ideal or proper lol, but this is what I've done for expanding myc onto more chips and/or carton...


Woodchips...I bring some water up to a simmer at most. Then I toss in the woodchips, turn off the heat, put a lid on it, and then let it sit at least over night if not a full 24 hours. Let the excess moisture drain away from the chips for a bit before I move them into a container or bag.


Egg carton...I simply soak egg carton in the "wood water" after the chips are done, the water that the chips were sitting in. Let em' soak for a bit, then when I pull them out I give it a good squeeze so they're just "moist" and not totally saturated/wet.




That's all I've done...I've had the stuff sitting in bags since January and there's no contam as far as I can see :shrug: . The myc is spreading nicely and thick.... though maybe a bit slow. I'm not sure what the typical growth rate is since this is my first time doing this (at least the first time doing it semi-properly lol).







-OM


.


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Invisiblebacillus
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Re: The Official Woodlovers Thread [Re: openmind]
    #25140136 - 04/15/18 05:21 AM (11 months, 1 day ago)

It depends on a situation, but usually I soak woodchips overnight. Egg cartons i just give a quick wash under a tap so that they don't saturate with water.

Generally, you have these options:
  • just hydrate
  • pasteurize (kill molds)
  • sterilize (kill molds and bacteria)


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InvisibleLizardWizard
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Re: The Official Woodlovers Thread [Re: bacillus]
    #25140154 - 04/15/18 05:44 AM (11 months, 1 day ago)

Actually, pasteurization kills bacteria as well, it's just the thermophilic bacteria that survive proper pasteurization, and those can only be of help to us in the colonization process and in substrate health.


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OnlineFerather
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Re: The Official Woodlovers Thread [Re: LizardWizard] * 2
    #25140250 - 04/15/18 08:00 AM (11 months, 1 day ago)

Just as a tip, pasteurization only works for a low-no starch, no free sugar and complex media.


If you pasteurized grain, all the endospores will hatch later, using the rich starch + macro-micro nutrient environment.
The same endospores can hatch on say coir, however in general they are less prolific than they are on starch.

If I add bacillus to enriched cellulose, it takes about 6-8 weeks to see a spot of slime, however it does colonize.
In the same timescale the mycelium which chiefly decays cellulose, should have "fully" colonized.

I have also tested a probiotic cellulose loving bacteria, from dry pet pellet food. The probiotic bacteria produced proper decay of cellulose.
Visibly the paper was falling apart in locations where there was no mycelium, mycelial BE and speed dropped considerably.


I have also tested some random archaea, from seaweed, it can survive the standard sterilization, and doesn't even produce endospores.
Mycelium cannot kill it, and it can be a primary decomposer. The archaea I have, can decay wood (phenols + cellulose).

Archaea is capable of fixating carbon, and fixating nitrogen, from air, but not both at once.
This can be beneficial (nitrogen) or an annoyance depending which one you get.


Example:

Wild Summer oyster benefiting from nitrogen fixating blue-green algae, on carbon rich nitrogen weak wood.

   


Extra links:

https://en.wikipedia.org/wiki/Nitrogen_fixation

Soil Amendments and Fertilizers (Worth a read).
The Carbon:Nitrogen Ratio (C:N).



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Edited by Ferather (04/15/18 08:18 AM)


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OnlineFerather
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Re: The Official Woodlovers Thread [Re: Ferather]
    #25140269 - 04/15/18 08:25 AM (11 months, 1 day ago)

Just so you all know, the mycelium which we commonly grow, does not fixate carbon or nitrogen, it does fixate oxygen however.
Your mycelium requires pre-fixated nutrient sources, these sources are usually made soluble via enzymes.

For example, cellulose is a carbon source, and ammonia is a nitrogen source.


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https://www.mediafire.com/folder/6rssmc08r8hwk/Journal - Ferather's Journal, guides, recipes and more [Downloadable]
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, same as above [Online]
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Re: The Official Woodlovers Thread [Re: Ferather]
    #25140735 - 04/15/18 12:36 PM (11 months, 1 day ago)

It's scary to think that some organisms would survive sterilization. I would argue it can't be called sterilization then.


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OnlineFerather
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Re: The Official Woodlovers Thread [Re: Adas]
    #25141120 - 04/15/18 03:20 PM (11 months, 1 day ago)

That is technically true Adas, any material that has extreme archaea-other either already in it, or added, would need much longer, or another method.
Common fungi, such as yeast or molds, common bacteria such as bacillus and e.coli, will be destroyed after 90 mins, but not everything.

This of coarse depends on the archaea-other and it's ability to decay the media, it may not colonize very much.

Lets say it survives, but doesn't really do much, and lets say it does not facilitate bacteria.
Ultimately it would not present as an issue, unless the opposite happens.


If it fixates nitrogen, and you have not enriched for example wood, that would be beneficial.
Another example of beneficial, other than nutrients, is substrate defenses.


--------------------
                   
--------------------
https://www.mediafire.com/folder/6rssmc08r8hwk/Journal - Ferather's Journal, guides, recipes and more [Downloadable]
https://www.shroomery.org/forums/postlist.php/Journal/350881 - Ferather's Journal, same as above [Online]
--------------------
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Re: The Official Woodlovers Thread [Re: Ferather]
    #25142292 - 04/16/18 01:07 AM (11 months, 15 hours ago)

Just curious if Cyan is supposed to look like this on agar? First pic is next to some cubensis myc. I thought they would look close. Do I have contaminants?



Spores are from a print I took last fall from fruits I found.


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Offlinemoricz
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Re: The Official Woodlovers Thread [Re: Baeomaze]
    #25142537 - 04/16/18 05:54 AM (11 months, 10 hours ago)

I bet on contam :\

But the center can be, transfer the center to carton then expand :smile:


Edited by moricz (04/16/18 05:55 AM)


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InvisiblebobwastakenS
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Re: The Official Woodlovers Thread [Re: moricz]
    #25142702 - 04/16/18 09:11 AM (11 months, 7 hours ago)

Quote:

moricz said:
If someone following our lil' adventure...

The 2 testubs are replaced to the cellar after two weeks of warming and a good dunking.

Conditions are:

12-13 C°
RH 95 %
lights on from 7:00 to 16:00
misting daily, gently.

I think if we are right we can see pins in a month.


so ... :popcorn:




What is your lighting set up for your cellar grows. Have you experimented with light intensity at all to see how it affects pinsets and fruit body development?


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Re: The Official Woodlovers Thread [Re: Poison Drink] * 1
    #25143023 - 04/16/18 12:47 PM (11 months, 3 hours ago)

Quote:

Poison Drink said:
Psilocybe cyanescens Ballard on SDA.



Three days after inoculating popcorn with agar wedge:



I have also inoculated some SDA with three to four year old prints, among which Psilocybe allenii. Hoping I can revive them after a growing hiatus. Fingers crossed!




The Ps. cyanescens grew rather slow on popcorn in my opinion. I believe the grains were a bit on the dry side. After full colonization, I transferred to egg cartons and wood chips. The wood chips were boiled for about 30-60 minutes and kept in a closed pot overnight. The next day I reheated the brown water to hydrate the egg cartons for a few minutes. I am using two different types of wood chips, separately. Growth is much faster now. The larger wood chips were inoculated five days ago and the smaller ones just three days ago. The egg cartons are kept in a ziploc bag in which a tiny gap provides GE.





Sadly I did not manage to germinate the four year old Ps. allenii print. Might try to revisit an old patch, but I haven't been there in years so I'm not very hopeful.


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Re: The Official Woodlovers Thread [Re: bobwastaken] * 1
    #25144711 - 04/17/18 03:38 AM (10 months, 30 days ago)

Just 5000 (or 6000) Kelvin LED bulbs, 60W equivalent. I have 2 of them. Don't think that is a key for them, maybe just the timing.

I 90% sure that the cycles do the trick. We will know in a month :smile:


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