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OfflineCaptainPuffy
Marbles Lost
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Registered: 06/08/11
Posts: 90
Last seen: 1 hour, 57 minutes
Re: The Official Woodlovers Thread [Re: rhizoRider]
    #28616665 - 01/10/24 05:30 PM (17 days, 5 hours ago)

Quote:

rhizoRider said: To last poster how are you starting off? Spores? Spawn? And most any wood will do just need azure to be well established and get cold to trigger fruits . It will test your patience but worth it




My plan is to go purchased LC - Agar - Grain Spawn - G2W Sterilized Wood - Outdoor pot with Wood/Coir/Spawn mixture topped with dirt and a strawberry plant.

I'll probably let the Wood/Coir mixture consolidate for a month or two before adding the dirt because the growing season starts late where I live. Shooting for Wood/Coir at mid-March and Dirt/Strawberry end of May.

I understand I may not get anything until the late fall or at all. I live in a place where they don't grow naturally so my plan is to take the pots inside before the deep freeze and bring them back out next spring.

The only thing I'm struggling with is what Wood to use for Spawn and in the pot. I'm thinking of a mixture of hardwood pellets, aspen shavings, apple shavings, and large aldar chunks.


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InvisibleNothingsChanged
Striving for Excellence
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Registered: 05/28/11
Posts: 10,142
Loc: North/Western WA Flag
Re: The Official Woodlovers Thread [Re: CaptainPuffy] * 4
    #28616925 - 01/10/24 09:07 PM (17 days, 2 hours ago)

Take some of your bigger, wood chips and hand, cut them up and chop them and smash them in the different sizes. Grain spawn will tear through the bigger chunks in no time.




--------------------


Edited by NothingsChanged (01/10/24 09:09 PM)


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OfflineFairyring
Ⱄⱄ.𓍊𓋼𓆏𓋼𓍊.ⱄⰔ
I'm a teapot


Registered: 12/18/23
Posts: 17
Loc: Alpha Centauri
Last seen: 1 day, 16 hours
Re: The Official Woodlovers Thread [Re: NothingsChanged]
    #28616960 - 01/10/24 09:51 PM (17 days, 1 hour ago)

Sorry I didnt see this thread before I made my own, however, is apple woodchips/sawdust viable?


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OfflineTweeq
Tweeq of Nature
Male

Registered: 06/07/18
Posts: 2,043
Loc: Netherlands Flag
Last seen: 7 hours, 38 minutes
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Re: The Official Woodlovers Thread [Re: Fairyring] * 1
    #28617050 - 01/11/24 12:31 AM (16 days, 22 hours ago)

Quote:

CaptainPuffy said:
I'm hoping to grow Azures and Cyans in pots. What type and size of wood should I be looking for? Should I pasteurize or sterilize?

I've found some aldar chunks, but they seem to be rather large (around an inch). Would I want to add cardboard or sawdust to fill in the gaps? I also saw some apple wood that seems to be smaller in size and cheaper.

Any advice is appreciated.
.




Any hardwood should work fine. 1 inch alder chunks sounds really good. Ime you don't need smaller bits or sawdust. The myc will jump from grain to wood just as easy. Apple is also good btw.

I would grow normal grain jars first and transfer those to (a bag of) sterilized wood chips. Once that is colonized you may choose to expand to more wood chips and/or spawn to a pot or patch in April/May.

Cardboard is not needed and old practice imo. Obsolete with clean grain spawn 2 wood.

Good luck!

PS. I wouldn't trust a bought lc to be 100 pct so test on agar and clean up if needed first.


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InvisiblerhizoRider
Mycorrhizally expanding
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Registered: 12/24/13
Posts: 1,915
Re: The Official Woodlovers Thread [Re: Tweeq] * 3
    #28617360 - 01/11/24 08:20 AM (16 days, 14 hours ago)

:crazy2: oh shit my warm ovoid tray pin is a INVADER species with spikey cap and musta been in cactus soil

I've got many other jars pinning and the bags with large chunks are pinning REAL pins looking like balls to start with blue ring towards top

I took some cool rhizo pics I'll add to this post update









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Offlinescapo
Scientist
I'm a teapot


Registered: 11/05/22
Posts: 268
Loc: PNW
Last seen: 2 hours, 42 minutes
Re: The Official Woodlovers Thread [Re: rhizoRider] * 8
    #28618203 - 01/11/24 11:07 PM (16 days, 4 minutes ago)

Psilocybe cyanescens blobs

A bag of chip spawn that started blobbing in the garage cased with peat/verm and now it's top blobbing and side pinning, we'll see!



Edited by scapo (01/11/24 11:14 PM)


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OfflineGeneric
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
Re: The Official Woodlovers Thread [Re: ghiajake]
    #28618804 - 01/12/24 03:06 PM (15 days, 8 hours ago)

Quote:

ghiajake said:
Think generationally. Indoor culturing is faster and more controlled, but outdoor culturing is a lot slower since it's on an annular cycle and subject to the elements. If you sector before fruiting, you'll have no idea if you eliminated the genetics that allow it to better naturalize to your specific microclimate.




That's how I'm thinking... I like what you've done, by selecting for traits and cloning and all, If we germinate spores then its really breeding for our conditions/bioregion.

So how would we best start from spores with as many strains as possible while still ensuring clean spawn?  I figured to select at least 4 strains; it seemed like a lot of agar work but not too much.  Any other ideas?

I also figured to let the original germination plate grow for a while for them to have time to mate, does this seem right?


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InvisibleBaba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
Trusted Cultivator
Re: The Official Woodlovers Thread [Re: Generic] * 4
    #28618809 - 01/12/24 03:09 PM (15 days, 8 hours ago)

700 pages of woody goodies. Rock on you lovely outdoor people :rockon:


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Offlinesmalltalk_canceled
Babnik
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Registered: 07/13/20
Posts: 2,862
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Re: The Official Woodlovers Thread [Re: Baba Yaga] * 1
    #28618969 - 01/12/24 05:27 PM (15 days, 5 hours ago)

When you pc wood furniture like a wooden bowl how long would you run it?

Like a adult sized wooden soup bowl

Is  furniture considered shorter pc times than grains?


--------------------
Willpower is the one true virtue



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Offlinesyri
Etard
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Registered: 09/27/23
Posts: 84
Last seen: 2 hours, 42 minutes
Re: The Official Woodlovers Thread [Re: smalltalk_canceled]
    #28619019 - 01/12/24 06:06 PM (15 days, 5 hours ago)

:lol:


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Offlineghiajake
Myco-Viking
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Registered: 01/10/13
Posts: 3,846
Loc: Indiana
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Re: The Official Woodlovers Thread [Re: Generic] * 2
    #28619076 - 01/12/24 06:56 PM (15 days, 4 hours ago)

Quote:

Generic said:
So how would we best start from spores with as many strains as possible while still ensuring clean spawn?  I figured to select at least 4 strains; it seemed like a lot of agar work but not too much.  Any other ideas?

I also figured to let the original germination plate grow for a while for them to have time to mate, does this seem right?




First and foremost, when it comes to WL you kinda need to remove the word "strain" from your vocabulary. A "strain" is a stabilized variant of a species, which even an isolate does not gain that title unless it consistently produces generations through spores that share the same taxonomical variation as the parent isolate.

IMO, yes the best way to go initially is multispore inoculation. Don't make plans about how many different variants of your MS culture you're going to play with because you want to select for the best traits from your first couple of year's harvests. What is the point of having multiple beds of different agar cuttings when you've never fruited any of them? Just make a couple beds with all the same MS culture for the first 2 years, take your clones, then plan on building new beds after with your cloned cultures.

As to your original plate. Yeah... Leave it alone until it's fully colonized, then inoculate a master jar or two of grain spawn with it and G2G for spawn expansion from there.

If you're asking me how to "start from spores with as many strains as possible while still ensuring clean spawn", that tells me you don't have much experience with lab work and should NOT dive into large agar projects. Get your lab work process down tight first, and you won't have to ask how to make "clean spawn".


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Offlinesyri
Etard
Female

Registered: 09/27/23
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Re: The Official Woodlovers Thread [Re: ghiajake]
    #28619078 - 01/12/24 07:04 PM (15 days, 4 hours ago)

Oh... Those pressure cookers:wonka:


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OfflineLand TroutM
Stranger
I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,076
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Re: The Official Woodlovers Thread [Re: Generic] * 1
    #28619083 - 01/12/24 07:11 PM (15 days, 4 hours ago)

Quote:

Generic said:
Quote:

ghiajake said:
Think generationally. Indoor culturing is faster and more controlled, but outdoor culturing is a lot slower since it's on an annular cycle and subject to the elements. If you sector before fruiting, you'll have no idea if you eliminated the genetics that allow it to better naturalize to your specific microclimate.




That's how I'm thinking... I like what you've done, by selecting for traits and cloning and all, If we germinate spores then its really breeding for our conditions/bioregion.

So how would we best start from spores with as many strains as possible while still ensuring clean spawn?  I figured to select at least 4 strains; it seemed like a lot of agar work but not too much.  Any other ideas?

I also figured to let the original germination plate grow for a while for them to have time to mate, does this seem right?



Don’t over think it
If you can see mycelium with your bare eyes they have likely mated


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OfflineGeneric
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
Re: The Official Woodlovers Thread [Re: ghiajake]
    #28619111 - 01/12/24 07:39 PM (15 days, 3 hours ago)

Quote:

ghiajake said:

As to your original plate. Yeah... Leave it alone until it's fully colonized, then inoculate a master jar or two of grain spawn with it and G2G for spawn expansion from there.

If you're asking me how to "start from spores with as many strains as possible while still ensuring clean spawn", that tells me you don't have much experience with lab work and should NOT dive into large agar projects. Get your lab work process down tight first, and you won't have to ask how to make "clean spawn".




Sure, my terminology's off, but with the idea of going from spores to agar, I saw one germ plate you mentioned that could just be sent to grain as is, and I was thinking you would surely want to do at least one transfer unless you get good at making good spore prints and streaks.  For me, I had to transfer away from bacteria.

I'll just combine everything when spawning


But when we germinate spores, and see mycelium from those points, are those not unique strains?  There's probably many definitions of 'strain'.  I like how in the plant world there are 'varieties'  and there are 'cultivars', or cultivated/traded varieties.  Can you name some strains of woodlovers do you know of that are selected for a certain trait as you say?


Edited by Generic (01/12/24 07:41 PM)


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Woodlovers Thread [Re: Generic]
    #28619117 - 01/12/24 07:48 PM (15 days, 3 hours ago)

Nothing wrong with making transfers away from potentially bacterial plates. Should still be lots of diversity in whatever selection you make.


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Offlinesyri
Etard
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Registered: 09/27/23
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Re: The Official Woodlovers Thread [Re: wavyedge]
    #28619288 - 01/13/24 12:17 AM (14 days, 22 hours ago)

Drink Fight and Fuck


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Invisiblethirdeyewild
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Registered: 11/01/13
Posts: 794
Loc: Flag
Re: The Official Woodlovers Thread [Re: TheDuder] * 6
    #28619586 - 01/13/24 10:34 AM (14 days, 12 hours ago)



--------------------


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OfflineBlopblop
Pantalones del Fuego!
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Registered: 05/08/23
Posts: 35
Loc: Olympia, Wa
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Re: The Official Woodlovers Thread [Re: thirdeyewild] * 3
    #28619783 - 01/13/24 01:30 PM (14 days, 9 hours ago)

10 jars of Subaeruginosa  spawn ready for wood chips. I’ve got a 3 day weekend which will be spent pc ing bags of alder chips. I know it’s pretty early but I’m pumped for springtime!


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Offlineghiajake
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Registered: 01/10/13
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Re: The Official Woodlovers Thread [Re: Generic]
    #28619942 - 01/13/24 03:35 PM (14 days, 7 hours ago)

Quote:

Generic said:

I was thinking you would surely want to do at least one transfer unless you get good at making good spore prints and streaks.


 

Why? For backup? Colonized grains last longer than agar in the fridge, but if you feel more comfortable transferring to a backup dish then go right ahead. For me, unless I'm putting the culture in an agar slant for long term storage I just keep a master grain jar in the fridge of each species I'm working with. Then when I pull the jar out to G2G I take a grain or two to agar for back up until I store another master jar.

BTW, just plan on needing to "get good" at every part of spawn production if you want to run with the big dawgs. :wink:


Quote:

Generic said:
But when we germinate spores, and see mycelium from those points, are those not unique strains?  There's probably many definitions of 'strain'.  I like how in the plant world there are 'varieties'  and there are 'cultivars', or cultivated/traded varieties. 




No, they are not a "strain" because even if only two spores mate the resulting culture is still just MS because both parent spores have the full genetic potential. Yeah, there are many "definitions" of the word strain, but just because someone who doesn't know what they're talking about calls something a strain doesn't make it so. The term "strain" is also used for plants, but mostly smaller organisms like bacteria/viruses. Since you know the plant ones the comparable term to strain is "cultivar", which is short for "cultivated variety". An isolate is also technically a cultivar since it is a "cultivated variety", but is does not earn the title "strain" until it has been stabilized to produce the same traits generationally. Multispore (MS) equals "native", and a regional mutation (like Hunter's summer azzies) equals a "variety" or "nativar". Hope that clears it up.


Quote:

Generic said:
Can you name some strains of woodlovers do you know of that are selected for a certain trait as you say?




Personally no, because I don't care enough to have learned any. But some of the other elder members here might be able to name some legit "strains" of WL's. If there are any that is.


Edited by ghiajake (01/13/24 03:52 PM)


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Offlineghiajake
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Registered: 01/10/13
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Re: The Official Woodlovers Thread [Re: ghiajake] * 1
    #28619950 - 01/13/24 03:43 PM (14 days, 7 hours ago)

Quote:

ghiajake said:
An isolate is also technically a cultivar since it is a "cultivated variety", but is does not earn the title "strain" until it has been stabilized to produce the same traits generationally.




This is why I will argue 'til the day I die that the "Enigma" P. cubensis mutation is NOT a "strain", since no one in the 6-7 years it's existed has ever been able to produce spores to create successive generations. It's just an isolated mutation that people have passed around so much the genetics mutated again due to sectoring.


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