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InvisiblerhizoRider
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Re: The Official Woodlovers Thread [Re: rhizoRider] * 2
    #28578274 - 12/11/23 08:31 AM (1 month, 16 days ago)





sorry for 3posts but I just got more pic space thx to woodlover brother here and wanted these shots included


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: rhizoRider]
    #28578888 - 12/11/23 04:35 PM (1 month, 16 days ago)

Rhizorider
This thread
And That thread
Are really cool reads and give a real good view into the description of P. ovoideocystidiata
Your culture reminded me of these
Yours are ovoid.


Edited by Land Trout (12/11/23 04:36 PM)


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OfflineAspectOfTheCreator
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #28578933 - 12/11/23 05:02 PM (1 month, 16 days ago)

Are woodlovers similar to pans, in that they prefer grain water agar to lma? This is my germ plate.



Maybe im just being impatient but the plate hasnt changed much the past 3 days and aside from taking a couple transfers to lma and gwa im unsure what to do from here. Theres just a bunch of nonexpanding dots. :shrug:


Edited by AspectOfTheCreator (12/11/23 05:11 PM)


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OfflineYoshiTrainer
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Re: The Official Woodlovers Thread [Re: AspectOfTheCreator] * 1
    #28578997 - 12/11/23 05:43 PM (1 month, 16 days ago)

Did you use a plate spreader?

I don't think they care much which agar you use, mine do fine on plain MEA.


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OfflineAspectOfTheCreator
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Re: The Official Woodlovers Thread [Re: YoshiTrainer]
    #28579018 - 12/11/23 05:53 PM (1 month, 16 days ago)

No? Whats a plate spreader? Im a simple man, I used a swab and sporeprint.


Edited by AspectOfTheCreator (12/11/23 06:47 PM)


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Offlineghiajake
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Re: The Official Woodlovers Thread [Re: AspectOfTheCreator]
    #28579078 - 12/11/23 06:50 PM (1 month, 16 days ago)

Quote:

AspectOfTheCreator said:
Are woodlovers similar to pans, in that they prefer grain water agar to lma?




It's not a Pans thing. Grain soak water works for the vast majority of all commonly grown fungal species. IMO is "primes" the myc for A2G transfers, but that may just be me being partial. I've used diluted GSW for all agar work for about 9 years now, at a 10:1 (water/GSW) ratio.


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: ghiajake]
    #28579257 - 12/11/23 08:19 PM (1 month, 16 days ago)

Quote:

Are woodlovers similar to pans, in that they prefer grain water agar to lma?



Ok, help me wrap my brain around this, or is this just something that’s been repeated so people belive it? I have a hard time believing that pans prefer grain soak water vs. malt that comes from grain. People have done great with grain soak water.

I’ve never had a problem with malt for any species, they all seem to do fine on it. The only agars I don’t like are water  and woodsoak.
Aspect, give those time, take a look under a stereo scope if you have one.


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OfflineYoshiTrainer
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #28579277 - 12/11/23 08:32 PM (1 month, 16 days ago)

A plate spreader allows you to put a couple drops of spore solution on your agar dish then spread them around. I asked because your entire plate looks covered.


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OfflineAspectOfTheCreator
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Re: The Official Woodlovers Thread [Re: YoshiTrainer]
    #28579572 - 12/12/23 05:59 AM (1 month, 15 days ago)

Quote:

YoshiTrainer said:
A plate spreader allows you to put a couple drops of spore solution on your agar dish then spread them around. I asked because your entire plate looks covered.




I streaked with a qtip


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OfflineAspectOfTheCreator
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Re: The Official Woodlovers Thread [Re: Land Trout] * 1
    #28579580 - 12/12/23 06:03 AM (1 month, 15 days ago)

Quote:

Land Trout said:
Quote:

Are woodlovers similar to pans, in that they prefer grain water agar to lma?



Ok, help me wrap my brain around this, or is this just something that’s been repeated so people belive it? I have a hard time believing that pans prefer grain soak water vs. malt that comes from grain. People have done great with grain soak water.

I’ve never had a problem with malt for any species, they all seem to do fine on it. The only agars I don’t like are water  and woodsoak.
Aspect, give those time, take a look under a stereo scope if you have one.




Its from my own experience but I have seen several people mention the same.


(The darker GWA plates are mislabeled, theyre supposed to be 12-2 and 12-4, not 11-2 and 11-4)

Ive had probably hundreds of Pan plates stall and get this crusty look on LMA. Every single time. I spent the better part of a year trying to remedy it with no success until I read an obscure thread and decided to switch agar types. Doesnt happen on gwa. Never.


Edited by AspectOfTheCreator (12/12/23 06:37 AM)


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Offlinecybesa
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Re: The Official Woodlovers Thread [Re: RescueU]
    #28579602 - 12/12/23 06:35 AM (1 month, 15 days ago)

That large one does NOT looks like a cyanescens, please be careful.


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InvisiblerhizoRider
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #28579605 - 12/12/23 06:40 AM (1 month, 15 days ago)

@ land trout, i see some of my pins resemble the small pins in those threads but I've not seen many resemble my grow variants well yet. I might raise temp n add case to half n see if it's happier and not aborting pins

Most voids are short and have fat large cap early pin stage. Mine are weird lol 😆


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Offlineghiajake
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #28579735 - 12/12/23 08:47 AM (1 month, 15 days ago)

Quote:

Land Trout said:
Quote:

Are woodlovers similar to pans, in that they prefer grain water agar to lma?



Ok, help me wrap my brain around this, or is this just something that’s been repeated so people belive it? I have a hard time believing that pans prefer grain soak water vs. malt that comes from grain. People have done great with grain soak water.

I’ve never had a problem with malt for any species, they all seem to do fine on it. The only agars I don’t like are water  and woodsoak.
Aspect, give those time, take a look under a stereo scope if you have one.





I'd guess the issue is too much malt extract in the recipe, making it WAY too nutritious. The stalling issue happens with GSW as well if it is not diluted. This is why I settled on a 10:1 ratio water/GSW, just enough nutrient to force rhizo growth but not enough to cause any stalling.


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Re: The Official Woodlovers Thread [Re: AspectOfTheCreator]
    #28579740 - 12/12/23 08:52 AM (1 month, 15 days ago)

Quote:

AspectOfTheCreator said:

Ive had probably hundreds of Pan plates stall and get this crusty look on LMA. Every single time. I spent the better part of a year trying to remedy it with no success until I read an obscure thread and decided to switch agar types. Doesnt happen on gwa. Never.




For us to figure it out we need your recipe. Preferably for both malt and GSW recipes. "Stalling" is usually one of two things, either not enough nutrient or too much.


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InvisiblerhizoRider
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Re: The Official Woodlovers Thread [Re: ghiajake] * 5
    #28579755 - 12/12/23 09:13 AM (1 month, 15 days ago)

:awedance:

The pretty one has spores dropping yayyyy

I will clone it into two or three mini stem butt cultures


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InvisiblerhizoRider
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Re: The Official Woodlovers Thread [Re: rhizoRider] * 3
    #28579759 - 12/12/23 09:17 AM (1 month, 15 days ago)





Mutant one cap went spiraling and then hit ground going back up. It's stem has mega fuzz healing the dry spot. Wild , it has gills but more sterile look to them so far. It's grown near inch upward after looking to abort. Tough mutant


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Re: The Official Woodlovers Thread [Re: rhizoRider]
    #28579760 - 12/12/23 09:17 AM (1 month, 15 days ago)

Love the blue nip! Those look awesome.


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: rhizoRider]
    #28579769 - 12/12/23 09:31 AM (1 month, 15 days ago)

Quote:

rhizoRider said:
Mine are weird lol 😆



Yeah they are.

@cybesa that one is Ps. cyanescens, maybe it got a touch of frost and it got a Lot of water. The darkened umbo can happen with maturity when saturated and frost, the orange brown stipe is typical when they’re saturated,  you can see the cortinate veil remnants with dark purple spore deposit and no ring which eliminates the possibility of it being a dangerous lookalike.

Thanks aspect, looks like you’ve got something that works for you. I haven’t worked that much with pans, have had them on grain, but always overthought the next step and had other stuff going on🤷🏻.


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OfflineAspectOfTheCreator
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Re: The Official Woodlovers Thread [Re: ghiajake]
    #28579962 - 12/12/23 01:11 PM (1 month, 15 days ago)

Quote:

ghiajake said:
Quote:

AspectOfTheCreator said:

Ive had probably hundreds of Pan plates stall and get this crusty look on LMA. Every single time. I spent the better part of a year trying to remedy it with no success until I read an obscure thread and decided to switch agar types. Doesnt happen on gwa. Never.




For us to figure it out we need your recipe. Preferably for both malt and GSW recipes. "Stalling" is usually one of two things, either not enough nutrient or too much.




Ive used everything from 0.5% to 2%.


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Offlineghiajake
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Re: The Official Woodlovers Thread [Re: AspectOfTheCreator] * 1
    #28580220 - 12/12/23 04:55 PM (1 month, 15 days ago)

Quote:

AspectOfTheCreator said:
Quote:

ghiajake said:
Quote:

AspectOfTheCreator said:

Ive had probably hundreds of Pan plates stall and get this crusty look on LMA. Every single time. I spent the better part of a year trying to remedy it with no success until I read an obscure thread and decided to switch agar types. Doesnt happen on gwa. Never.




For us to figure it out we need your recipe. Preferably for both malt and GSW recipes. "Stalling" is usually one of two things, either not enough nutrient or too much.




Ive used everything from 0.5% to 2%.




0.5%-2% of what, GSW or malt? That's not really a recipe, if you want us to answer the question we need more info first. Also, what type of grain, and how dark is your GSW? Let me give mine as an example.

Nutrient Broth:

9 or 10 parts water, depending on how thin I want the nutes.
1 part GSW

2 grams of agar powder for every 100mL of nutrient broth.

Let's say I'm pouring 100 plates at 10mL per plate, that means I need 1L of liquid agar mix plus a bit extra just in case. So I'll make up 1.1L, 1000ml of water and 100ml of coffee-filtered GSW. That's 9% GSW, and that's as thin as I ever make the nutes in my agar. Others may do it differently, but as far as I've experienced if I go any thinner on the nutes the myc will starve out, regardless of species.


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