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Offlinetrubblesome
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Registered: 11/09/19
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Re: The Official Woodlovers Thread [Re: Hindsight]
    #27280273 - 04/24/21 10:07 AM (2 years, 8 months ago)

yo woodlover friends, any tips/tricks for starting ovoids on agar? do i need a different recipe than LME? having trouble after several attempts, I check them under scope each time and the spores are there! they just don't want to grow.

first attempts were swiped from print, then i made a spore syringe, nada. next step I'm trying today was placing the syringe in the refrigerator for the last few weeks. not super confident though so thought I'd ask here!


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InvisibleWrightii
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Registered: 05/15/20
Posts: 216
Re: The Official Woodlovers Thread [Re: trubblesome]
    #27280300 - 04/24/21 10:41 AM (2 years, 8 months ago)

Hi people :smile:

Its my first time trying to grow psilocybe cyan outdoors this year. I've made it from sporeprint to a medium sized tub with inoculated beech chips so far. Now my question is, when I lay the beds tomorrow, do I add more un-inoculated beech chips? And if so, what is a good ratio? I see 1:5 comming by when reading, but thats only for the first transfer from grain to chips right? And should I also pasturize these, or is an overnight soak enough?
Or should I just spread out the allready colonized chips in to a bed, without adding anything new at all. 

Cheers!
-Wii


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The Official Hericium Thread


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OfflineBU4O
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Re: The Official Woodlovers Thread [Re: Wrightii] * 1
    #27281003 - 04/25/21 01:54 AM (2 years, 8 months ago)


Not my bed, it's a project of a friend of mine.
P.A. Ovoid strain,clone from stem buds taken from my garden bed last year.
Good luck all!


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OfflineShroomhunts
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Re: The Official Woodlovers Thread [Re: CHUCK.HNTR]
    #27282000 - 04/25/21 09:42 PM (2 years, 8 months ago)

I would just let them do their thing, when fruiting happens they will spread spores


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You never kno


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OfflineHaywire
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Re: The Official Woodlovers Thread [Re: Shroomhunts]
    #27282214 - 04/26/21 04:17 AM (2 years, 8 months ago)



after at least 20 substrates, this is the first one the baeocystis is actually is colonizing.
substrate is sterilized mulch from a bag.
spawn is wbs inoculated with liquid culture.
I've tried straight sawdust, supplemented with manure, woodchips, mulch straight out of the bag, ...

difficult fuckers!!!


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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InvisibleCHUCK.HNTR
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Registered: 09/30/19
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Re: The Official Woodlovers Thread [Re: trubblesome]
    #27282535 - 04/26/21 09:34 AM (2 years, 8 months ago)

Quote:

trubblesome said:
yo woodlover friends, any tips/tricks for starting ovoids on agar? do i need a different recipe than LME? having trouble after several attempts, I check them under scope each time and the spores are there! they just don't want to grow.

first attempts were swiped from print, then i made a spore syringe, nada. next step I'm trying today was placing the syringe in the refrigerator for the last few weeks. not super confident though so thought I'd ask here!




I had a really tough time getting ovoids started of agar. I had to keep trying with different prints and even then the plate was in the incubator for months. Sometimes I would just add more spores to an old plate as long as it was still free of contamination.
Sorry I don’t have advise I had a lot of trouble with P. cyanescens too and the prints were fresh from wild fruit.
Once you get the culture going they are strong as can be and form rhizomorphic growth very quickly.
Unlike some cubensis that take lots of work to attain good rhizomorphs.


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"What is the practical application of a million universes?" -Alan Watts
:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:


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OfflineHaywire
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Re: The Official Woodlovers Thread [Re: CHUCK.HNTR]
    #27283353 - 04/27/21 01:41 AM (2 years, 8 months ago)

I've used regular MEA and it worked fine.
spores germinated quickly too.


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: Haywire]
    #27283582 - 04/27/21 08:03 AM (2 years, 8 months ago)

With ovoid I bought a print that I’m pretty sure was dead and then got a print from a guy off Instagram and bingo, it worked. 
Haywire what type of bagged mulch?  Like nature scapes? Or a compost or bark mulch? 
My ovoid patch is finally poppin!  We’ve had such hot a dry weather I was really doubting it would happen this season, I’ve been watering it a lot, but we got a couple days of rain and now things are really getting going.


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OfflineHaywire
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #27283941 - 04/27/21 01:54 PM (2 years, 8 months ago)

I'm not in the US so brands are different.
It says on the bag 'mulch'.
it smells of pine and just looks like it's been decomposing for a while.


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflinePs.NoName
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Re: The Official Woodlovers Thread [Re: Haywire] * 3
    #27286771 - 04/29/21 03:38 PM (2 years, 8 months ago)

First sign of fruits from outdoor ovoid patch started about 22 months ago. Spores -> pf cake -> wood chips.



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Set me off, see what I'm worth. Turn me on, I go berserk.



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Offlinetrubblesome
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Re: The Official Woodlovers Thread [Re: Ps.NoName]
    #27286843 - 04/29/21 04:40 PM (2 years, 8 months ago)

thanks y'all for the ovoid starting info, guess I'll keep crackin and just look for a new print in the meantime.


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: trubblesome]
    #27286856 - 04/29/21 04:51 PM (2 years, 8 months ago)

Ovoids are great once you get them going, almost hard to kill in my experience.


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Offlinesmalltalk_canceled
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #27286879 - 04/29/21 05:08 PM (2 years, 8 months ago)

Luteoviridis on rye:



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OfflineBU4O
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Re: The Official Woodlovers Thread [Re: smalltalk_canceled]
    #27287682 - 04/30/21 10:01 AM (2 years, 8 months ago)


My friends bed is going my beds aren't fruiting for now :justdontknow:


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InvisibleSpacetuna
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Re: The Official Woodlovers Thread [Re: BU4O]
    #27287905 - 04/30/21 01:54 PM (2 years, 8 months ago)

I have a yellow slime mold in one of my beds that seems to have taken dominance and killed the ovoid mycelium. Shit poped up last year and ate some fruits at the end of the season now I have no bright white mycelium in this area of the bed.  Any way to kill the slime mold ?


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OfflineBU4O
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Re: The Official Woodlovers Thread [Re: Spacetuna]
    #27288446 - 04/30/21 10:27 PM (2 years, 8 months ago)

No way to kill it... I have one cyan and one subs plastic tubs that are almost killed by it... Your only option is sun and drying out the entire bed and the woodchips...


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OfflineTyperwritermonky
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Re: The Official Woodlovers Thread [Re: Ps.NoName]
    #27292988 - 05/03/21 08:52 PM (2 years, 8 months ago)

Quote:

Ps.NoName said:
First sign of fruits from outdoor ovoid patch started about 22 months ago. Spores -> pf cake -> wood chips.






must feel so good!  My patch is progressing well, I'm hoping since they aren't supposed to fruit for another 8 months that I'll get some fruits this year - but if not, two years.  I can tell the mycelium is totally active and growing, it's just a matter of if they'll grow and settle enough to produce fruits.  I have a feeling I'll at least get a few small ones, but hoping for a sweet flush!


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: Typerwritermonky] * 2
    #27293159 - 05/03/21 10:20 PM (2 years, 8 months ago)

Wanted to give some updates on some R and D I’ve worked on for sawdust blocks.  So I’m working with red alder, I bought it by the yard from a landscape supply complany, they sell it for mushroom growing, as in if you call them and ask for alder sawdust they say you mean for growing mushrooms.  It looks like a mix of what comes off the band saw and the fines that get screened on a chipper belt so there’s some bigger chunks in there, but definitely not chips.  I haven’t pasteurized or run any of it in a canner.  I haven’t counted but I’m around 200 bags, out of those I’ve lost less than a dozen to trich.  The only ones I’ve lost were azzies.  I had to start azzie from spores this fall.  The ovoids cyans and subs I’ve started from slants or plates that were sitting in my fridge for about 12 months.
One theory I’m theorizing is that I buy his stuff and it’s in a 100 yard pile or so that they scoop my two yards out of, and it’s hot when they load it into the trailer.  All of my subs ovoids and cyans were grain spawned to the sawdust when it was peaty damn fresh and likely loaded with thermophilic bacteria, which I remember reading Stamets talking about being beneficial to fungi, and the reason why substrates are pasteurized instead of sterilized.  The azzies took longer to get to good grain spawn so possibly the bio community of my sawdust pile changed and I started getting trich outbreaks.  This is just a theory, I’m not a good scientist so don’t take this as a thesis, but just something to think about and observe in the future. 
Also I tested unicorn bags vs. simple gusseted poly bags from plastic bags.com or something like that.  I learned there is more to gas exchange than I believed, and the inicorn bags out preformed the other poly bags.  I just zip tie them, some I zip tied close around a wad of polyfill which did better than bags that were simply zip tied.  However when I saw the unicorn bags or polyfill vented bags running faster I removed the zip tie and just did a single fold over and that seems to be working just fine.  Also as far as the trich in my azzies, the unicorn bags were least affected, almost all the poly bags got trich, and only one or two unicorn bags got it.  I’ve been using the 14a bags. 
Hope this helps, or at least gets the wheels turning.  Everything I’ve spawned to the orchard clerk bank and flower gardens is doing really well, I did have one plum tree die, so we replaced it with an apricot. 
Oh!  And the first bags that got trich I just spawned around an apple tree I planted and the shits looking good!

The one pic is literally an earth worm in my sawdust block and the fungi looks to be thriving.


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OfflineHaywire
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #27293312 - 05/04/21 03:22 AM (2 years, 8 months ago)

I'm happy that no treatment works for you.
tried it twice now and both of them triched out heavily :frown:


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Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineTyperwritermonky
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Re: The Official Woodlovers Thread [Re: Haywire]
    #27294576 - 05/04/21 07:52 PM (2 years, 8 months ago)

Hey guys I have a question, maybe there's a better place to ask it, but I'll ask here.  Are there any particular toxins or nasty bacteria that can be on or in woodlovers that are natural patches?  Especially any that might survive the drying period at a medium heat for several days with air drying until it's cracker dry?

I consumed probably.. .4-.5g of dried p. alleni over the course of 2 and a half months or so in microdoses.  Absolutely confirmed, looked over multiple times, etc.  So I know it's not those, but is there any sort of bacteria or toxin like I said that can cause health issues that could still be active on the dried mushrooms even after a good while?

I've had some strange health issues after receiving the 2nd Pfizer covid shot, and my liver started having very high enzyme levels and I had to be hospitalized within 48 hours of getting the shot.  every possible test came back negative, so they have no idea what's caused it.  I'm quite sure that it's the shot, but trying to rule everything out.

I hadn't microdosed as well for about two weeks before getting my second shot, but is there anyway that these microdoses could be account for the liver issues I experienced?  They are confirmed p. alleni from myself and TI's on this website... so I know it's not the mushroom themself, but could there be something piggybacking here that anybody has heard of?  Like I said, I'm just trying to rule everything out.


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