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Offlinewxorx
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Re: The Official Woodlovers Thread [Re: NothingsChanged]
    #27235229 - 03/03/21 01:11 PM (3 years, 26 days ago)

Guys, please take a look at my plates below.
So, I've made several attempts and it seems my prints were a bit old or my agar dry, I don't know, but they did not wanted to germinate.
In fact, I don't know how old the prints are, cause only one was dated 2017 (P.alenii), presumably the others are more fresh.

Finally I decided to hydrate them in syringes with distilled water for some days and tried again, that was on Feb 07.


Something appeared on the top left plate after few days (12/02) but then it turned to be some nasty thing:


The oldest known one, P.alenii, in the bottom right, caught some bacteria and was discarded at some point, and in the picture below is replaced
with new fresh plate with P.azurescens spores, so don't mind the bottom right one.

This is how they look today:



I've made transfers already from sections looking good to me (the ones with holes), but too early to post pics of them. What you guys think about them?
To my inexperienced eye, both ovoid plates looks somewhat good, but what you think about the azzie (top left) and subaeruginosa (center)?

Thank you!


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void **

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Offlinewoods
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Re: The Official Woodlovers Thread [Re: wxorx] * 1
    #27235367 - 03/03/21 03:06 PM (3 years, 26 days ago)

Hey Friends,

My first agar to grains project is working well. I started with azzy and cyan tissue put to agar in November and December. In January to early Feb, I put agar to grains. Some of the mycelium growth into the grains has been robust, some not as much. I have a heating pad on low under the box with jars in it, and the box is in my home office.

I have a few questions.

Here's a jar with agar put to grain on Jan 13, and mycelium growth is there but not robust and/or seems to have stalled. I also notice a brown liquid in bottom of jar. Is this likely contaminated? Should I give it more time or just put to chips outside and see what happens?







Other jars with agar put to grain on Jan 21 look much better but still have some areas without mycelium growth as shown below. Are these ready to be put to wood chips or should I allow more mycelium growth to occur first? When are jars ready to move colonized grain to wood chips?









Thanks!

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Offlinetututotutut
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Re: The Official Woodlovers Thread [Re: woods]
    #27235382 - 03/03/21 03:13 PM (3 years, 26 days ago)

liquid at bottom of jars is bacteria nevermind


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yeah keep telling yourself that's voluntary

Edited by tututotutut (03/03/21 03:25 PM)

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InvisibleBaba Yaga
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Registered: 09/13/20
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Re: The Official Woodlovers Thread [Re: wxorx]
    #27235388 - 03/03/21 03:18 PM (3 years, 26 days ago)

Quote:

tututotutut said:
This is how they look today:



I've made transfers already from sections looking good to me (the ones with holes), but too early to post pics of them. What you guys think about them?
To my inexperienced eye, both ovoid plates looks somewhat good, but what you think about the azzie (top left) and subaeruginosa (center)?

Thank you!




Well you got growth, that's good. The Azzies and Subs definitely do look like they need some cleanup work. Show us some photos of your most recent
transfers when they have grown out a bit and maybe photograph each plate individually for better visibility.

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OfflineAdas
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Re: The Official Woodlovers Thread [Re: tututotutut]
    #27235400 - 03/03/21 03:24 PM (3 years, 26 days ago)

Quote:

tututotutut said:
liquid at bottom of jars is bacteria




Not with Ovoids though. They do it every time.

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InvisibleBaba Yaga
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Re: The Official Woodlovers Thread [Re: tututotutut]
    #27235401 - 03/03/21 03:25 PM (3 years, 26 days ago)

Quote:

tututotutut said:
does this azurescens plate look right? It's very thick, has me a little worried Im used to whispy cube rhizo





Can't really tell from that photo, can you get a better shot, I know it's difficult with those containers.

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Offlinetututotutut
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Re: The Official Woodlovers Thread [Re: Baba Yaga]
    #27235408 - 03/03/21 03:29 PM (3 years, 26 days ago)



--------------------
yeah keep telling yourself that's voluntary

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InvisibleBaba Yaga
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Re: The Official Woodlovers Thread [Re: Baba Yaga] * 1
    #27235418 - 03/03/21 03:37 PM (3 years, 26 days ago)

Two more subaeruginosa / coir shoeboxes are fully colonized, will add some fermented wood chips soon.




Two bigger tubs from earlier got topped off with some pasteurized woody potting soil.



They seem to be doing well so far  :dancer:

Edited by Baba Yaga (03/03/21 03:46 PM)

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InvisibleBaba Yaga
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Re: The Official Woodlovers Thread [Re: tututotutut]
    #27235441 - 03/03/21 04:01 PM (3 years, 26 days ago)

Quote:

tututotutut said:





That plate looks alright, but TBH I never worked with azzies so I'm not too sure about the fuzziness, just a general observation, hopefully
someone else with first hand experience will chime in.

So? don't your work with a SAB?

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Offlinetututotutut
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Re: The Official Woodlovers Thread [Re: Baba Yaga]
    #27235474 - 03/03/21 04:34 PM (3 years, 26 days ago)

ahhhh haha nope I've been a schmuv schmuck lately. Who knows maybe I'll switch back to sab in the future, I believe its working for me now. There's been so many other factors that I've been tweaking along the way. I don't have the money for flowhood at the moment.


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yeah keep telling yourself that's voluntary

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InvisibleBaba Yaga
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Re: The Official Woodlovers Thread [Re: tututotutut] * 1
    #27235495 - 03/03/21 04:50 PM (3 years, 26 days ago)

Ah ok, I thought that was just a plastic bag, all good then
Schmuv boxes seem to work very well for some peeps. Sweet:thumbup:

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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: Baba Yaga] * 2
    #27235703 - 03/03/21 06:14 PM (3 years, 26 days ago)

A lot of my wood lovers are real tomentose on agar and grain, and don’t get ropey until after they are on the wood for a bit.

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Offlinetututotutut
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #27235713 - 03/03/21 06:23 PM (3 years, 26 days ago)

I'm going to put that dish on grains soon. Will report back. My lions mane and stipticus are definitely running on agars now, excellent stuff. Might be slow to get those into fruition at first, very focused on cubes right now


--------------------
yeah keep telling yourself that's voluntary

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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: tututotutut] * 3
    #27236191 - 03/03/21 11:35 PM (3 years, 25 days ago)

I’m just a step ahead of some of you guys as far as building spawn so I figured I write something up cause you might have some questions as your wood lovers mature into the next steps of the process.  As far as getting these things to fruit I am not very experienced.  It’s natural anytime your doing something for the first time that you may feel unsure of your work or something.  These wood lovers are very hardy and are very different from cubensis, but there’s a lot of similarities and it’s really fun to grow these.
Here is a picture of a couple handfuls is saw dust inoculated with an agar plate.  I transferred the agar to the sadust with a dirty bare hand back in december the bag is a dirty used ziplock.

That’s not my standard was of making spawn at all, it takes too long, but you could use that spawn a tub of wood chips.  I just want to show you how “tough” they are.  I also have sawdust spawn bags with worms In them

I didn't add worms on purpose, but my pile of sawdust is just in my yard and I think they just lay their eggs in the pile or something, but they don’t seem to be bothering the myc or even eating the grain.  So who knows, the fungi might be getting a benefit from the worms.  That cyan bag was spawned with grain spawn on 2/20.
Here’s some agar pics of the azurescens I’m working with.Formula: 0

The first pic is a t2 the only reason i made a t2 is the t1 had a spot of bacteria so I made a few plates to clean it.  That one isn’t going as fast as just some random tomentose plates I had.  The second plate it the multispore I took the transfers from, and the third plate is another multispore.  I don’t worry about isolating these things too much, I think I read someone say, I think it was Moricz, say they didn’t so I don’t.
It’s amazing how clean these things are for being out in the wild and who knows what until the cap gets put to foil.  The plates were inoculated using a loop stroke from a print and streaked to a plate inside a sab.  The prints were from the wild this past fall. 
So here’s that t2 on grain,
 
pretty dense and cottony not what I’d want to see in cubensis spawn.
The jar on the right has been shaken, the jar on the left has not.  The grain is oats prepped by a multi day soak and brisk boil PCd and inoculated on 1/24.  Seems pretty slow to me, but I’m not worried.  I’m just getting some of these azzies spawned to sawdust, mostly just multispore plates I had put to grain.
Here’s where it starts to look cool.  So my plan is to put these block throughout our property, we have creek bank lawn, pasture rhododendron, gardens and good irrigation just all kinds of places to plant this stuff.  We just moved here last summer so I’m trying to just make as much spawn as I can so the stuff can really settle into  this place as we do. 
Here’s what I did with the plates from the pictures above.
 
I’ve got plenty of plates, and plenty of sawdust, who knows maybe worms will hatch in these and eat the agar, or maybe they make little spawn bags.

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OfflineHaywire
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Re: The Official Woodlovers Thread [Re: Land Trout] * 1
    #27236248 - 03/04/21 01:07 AM (3 years, 25 days ago)

Nice, I tried that untreated sawdust a second time in a tote and so far no colours are popping up.
It's heating up big time though (30°C).
Maybe it was the crushed corn cob that messed it up last time.


--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches

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OfflineEdmunter
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Re: The Official Woodlovers Thread [Re: Haywire]
    #27236371 - 03/04/21 05:16 AM (3 years, 25 days ago)

Does anyone ever go straight to woodchips with an LC?

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OfflineEugene Gesuale
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Re: The Official Woodlovers Thread [Re: Edmunter]
    #27236416 - 03/04/21 06:19 AM (3 years, 25 days ago)

I hear that if your lc has wood in it it will take better that way


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OfflineLand TroutM
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Re: The Official Woodlovers Thread [Re: Edmunter]
    #27236542 - 03/04/21 08:33 AM (3 years, 25 days ago)

@edmunter I tried that last year and it didn’t work well.  It would be nice to find a way to make that work though.

Edited by Land Trout (03/04/21 08:35 AM)

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Offlinemadyogi
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Re: The Official Woodlovers Thread [Re: Land Trout]
    #27236550 - 03/04/21 08:35 AM (3 years, 25 days ago)

Quote:

Land Trout said:
I’m just a step ahead of some of you guys as far as building spawn so I figured I write something up cause you might have some questions as your wood lovers mature into the next steps of the process.




Nice write up, Trout.  Your property sounds awesome!  What zone are you in?

For anyone interested, I'm detailing my first attempt at making some Azzie beds in zone 7b on this thread (Azurescens Advice?) and this journal entry (My Outdoor Azurescens Experiment).  Just put 7 jars of Azzie on Oats to a variety of wood subs yesterday.


Edited by madyogi (03/04/21 08:36 AM)

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OfflineDabadava
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Re: The Official Woodlovers Thread [Re: Edmunter]
    #27236753 - 03/04/21 10:40 AM (3 years, 25 days ago)

Quote:

Edmunter said:
Does anyone ever go straight to woodchips with an LC?




It's sawdust, not woodchips, but I think it's the same.


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