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Offlinenoodles-uk
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Re: The Official Woodlovers Thread [Re: Adas]
    #26825421 - 07/15/20 02:46 PM (26 days, 1 hour ago)

:thumbup:

can I put sluggo actually on the patch or just around it?


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InvisiblebobwastakenS
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Re: The Official Woodlovers Thread [Re: Adas] * 1
    #26825845 - 07/15/20 07:39 PM (25 days, 20 hours ago)

Quote:

Adas said:
Maybe you should start patches in the winter. The mycelium needs time to get into the pores of the wood, there it can be dormant for months apparently. Use larger pieces of wood, that may also help. You also need a suitable spot that's more humid than usual.
Also, a good plant cover helps retain a lot of moisture.

Best of luck!




:whathesaid: Build sterile spawn in the summer months and make your beds at the start of the season or when temps average 70f or below. The following season you should have fruits.


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OfflineFerather
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Re: The Official Woodlovers Thread [Re: bobwastaken]
    #26826414 - 07/16/20 07:06 AM (25 days, 8 hours ago)

I prefer the buried cake method.


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OfflineShroomhunts
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Re: The Official Woodlovers Thread [Re: Ferather] * 1
    #26826422 - 07/16/20 07:11 AM (25 days, 8 hours ago)

Quote:

Ferather said:
I prefer the buried cake method.



Yeah buried cakes are the eay to go. Stupid ez.


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Re: The Official Woodlovers Thread [Re: Shroomhunts] * 2
    #26827101 - 07/16/20 02:30 PM (25 days, 1 hour ago)

Hey guys,

After playing with Semperviva, first experience with a (semi)woodlover - Caerulescens :









--------------------
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OfflineFerather
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Re: The Official Woodlovers Thread [Re: Shroomhunts]
    #26827138 - 07/16/20 02:46 PM (25 days, 1 hour ago)

Here's how I see it (no offense):

You can use the benighted method, which is throwing perfectly good spawn into low pH wood, with basically no nitrogen whatsoever, and get 10 mushrooms in 2 years.
Or you can educate yourself with what's missing in wood, why the low pH is bad, what inhibitory materials are, what phenols are, and so on.

If you want to skip that, look at gourmet recipes with bran and calcium carbonate, or WL-Tek.

----

Here is a test most people can do, hydrate 25g of wood pellets, and 25g of paper pellets, and leave them to get mold.
You will notice the paper pellets don't appear to germinate anything, even after a few years.

It's complicated, however in short the soluble phenols-other have been removed.

https://en.wikipedia.org/wiki/Acid-free_paper

----

If you add wheat bran to the paper pellets, then test it, it will germinate various organisms.
This is due to the starch in the bran, which is used to germinate and feed.

This is why I use fertilizer, it's just macro-micro nutrients.

https://www.ncbi.nlm.nih.gov/pmc/articles/

----

@ Aendir, very nice.


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OnlineCelestialexplorer1
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Re: The Official Woodlovers Thread [Re: Ferather]
    #26827236 - 07/16/20 03:39 PM (25 days, 10 minutes ago)

Thank you for that post ferather. I didn’t know about that, I’m pretty new to all this and there’s no telling how long it would have taken me to find this out since I’m in the process of starting some outdoor and indoor grows.

Can I ask what type of fertilizer you use. I’ve been doing research on fertilizers effects on mushrooms and actually just got some, micro-life


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Edited by Celestialexplorer1 (07/16/20 03:41 PM)


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OfflineBobbit
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Re: The Official Woodlovers Thread [Re: Ferather]
    #26828193 - 07/17/20 04:40 AM (24 days, 11 hours ago)

Quote:

Ferather said:
There's not so much on the lemon tek and citric acid specifically, however try this (the reaction).

Link 1, Link 2, Link 3. Google Search: psilocybin dephosphorylation



Dude, there are quite a few newer papers on extraction, and it appears that most Psilocybe species produce phosphatases which is why the generally agreed method of preservation is to dry as quickly as possible. Any aqueous solution H2O, ethanol, acetic acid, etc. all pull the present phosphatases also, making them less stable due to dephosphorylation to psilocin, which is very easily oxidised. . .

This is the main reason I asked to to back up your claim, as if it were as easy as adding citric acid to both dephosphorylate psilocybin and make psilocin a more stable salt (psilocin citrate), would be ABSOLUTELY AMAZING. . .

It still seems that non-aqueous methanol extract to separate actives from phosphatases Is potentially the most likely pathway. . . Would love to be proved wrong. . .


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OnlineAdas
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Re: The Official Woodlovers Thread [Re: Bobbit]
    #26828221 - 07/17/20 05:26 AM (24 days, 10 hours ago)

Keep in mind that certain solvents and pH ranges can render certain enzymes inactive (change their tertiary structure making them useless). So it's likely true for the phosphatase as well, we just don't know what exactly, but IMO it's safe to say that low pH or concentrated alcohols inactivate it on contact.


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OfflineBU4O
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Re: The Official Woodlovers Thread [Re: Adas]
    #26828238 - 07/17/20 05:43 AM (24 days, 10 hours ago)

So my vodka with all the dried libs and allenii from 2019 is useless, I am so so unhappy right now :cryariver:  :crymeariver:  :bestcry:


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OnlineAdas
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Re: The Official Woodlovers Thread [Re: BU4O]
    #26828239 - 07/17/20 05:44 AM (24 days, 10 hours ago)

Ehm... Quite the opposite actually :rofl:

But you should try anyway, I could be wrong.


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OfflineBU4O
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Re: The Official Woodlovers Thread [Re: Adas]
    #26828326 - 07/17/20 07:09 AM (24 days, 8 hours ago)

I did leave it in cold dry place away from light so it can extract more...
I have plenty of dry mushrooms various kinds so I did want to store them that way...


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OfflineFerather
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Re: The Official Woodlovers Thread [Re: BU4O]
    #26828459 - 07/17/20 08:53 AM (24 days, 6 hours ago)

@Bobbit, no you are correct, I did say in my original post about the normal-better method, with alcohol.
@Celestialexplorer1, checkout my "Growing Mushrooms" post in my journal.

https://www.youtube.com/watch?t=2256&v=y7mdAS7KlRw


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Commander: [Cygwin + Cmder] - Customize Android, Setup VirtualBox, plus more [2019]


Edited by Ferather (07/17/20 09:05 AM)


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Re: The Official Woodlovers Thread [Re: Ferather]
    #26830064 - 07/18/20 01:24 AM (23 days, 14 hours ago)

@Ferather What do you mean when you say ‘inhibitory materials’?


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OfflineBobbit
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Re: The Official Woodlovers Thread [Re: Adas]
    #26830237 - 07/18/20 06:16 AM (23 days, 9 hours ago)

Quote:

BU4O said:
So my vodka with all the dried libs and allenii from 2019 is useless, I am so so unhappy right now :cryariver:  :crymeariver:  :bestcry:



Remains to be seen. . . Testing is in order. . .

Quote:

Adas said:
Keep in mind that certain solvents and pH ranges can render certain enzymes inactive (change their tertiary structure making them useless). So it's likely true for the phosphatase as well, we just don't know what exactly, but IMO it's safe to say that low pH or concentrated alcohols inactivate it on contact.



Many phosphatases are alkaline so lowering pH isn’t necessarily a positive. . . Phosphatases seem to often have denaturation temps of 85+°C also, and become more active at temps above room temp and heat denaturing will kill psilicybin before the phosphatases. . .


That is why I was hassling Ferather for real info on psilocin citrate (or any salt that is stable) so the phosphatases are not an issue as psilocyn has no phosphate group. As long as there is a stable salt, it may be extractable. . .

Some of the early research suggests that acidic (acetic) extracts seem to accelerate dephosphorylation, resulting in no actives. . .

I wish I had paid more attention in Bio and Chem. . .

And should prolly end up back on topic. . .


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OfflineFerather
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Re: The Official Woodlovers Thread [Re: Bobbit] * 1
    #26830299 - 07/18/20 07:39 AM (23 days, 8 hours ago)

@Bobbit, your body will dephosphorylate psilocybin into psilocyn, at this point is can pass into the bloodstream and brain barrier.
The lemon tek, makes the psilocybin more susceptible to dephosphorylation (like stomach acid), speeds it up.

It can only speed up the conversion process, not increase potency (total amount).

----

Extraction Procedures: http://www.pbso.org/qualtrax/QTDocuments/1968.PDF

----

@anon1978, https://www.sciencedirect.com/science/article/abs/pii/S0964830512002843
Various materials-chemicals in wood are anti-fungal and can inhibit growth.

Good examples are fresh pine wood, or cedar wood.


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OfflineBobbit
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Re: The Official Woodlovers Thread [Re: Ferather]
    #26831051 - 07/18/20 03:49 PM (23 days, 59 seconds ago)

Your links speculate where in the body this happens, yes. . . Not looking to ‘strengthen’. . .

Internal dephosphorylation doesn’t really help with extractions though :shrug:

It would be really nice to be able to have a stable solution containing actives for microdosing. . .

This is why I’m keen to keep asking the questions though there are many looking and no-one really finding consistent results. . .

As cool as it would be to have a dose in a few drops, just being stable in solution is enough. . .

For now it seems that anhydrous methanol will pull all the SillySimon, and ignore the phosphatases. . . The only consistent ‘easy’ tek for the kitchen chemist. . . I’m thinking trying this then evaping all the methanol, then adding ethanol for tincture. . .

Maybe adding a peptidase to eat the enzymes?

This convo appears periodically, yet peters out before anything concrete lands. . .


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OnlineAdas
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Re: The Official Woodlovers Thread [Re: Bobbit]
    #26831078 - 07/18/20 04:04 PM (22 days, 23 hours ago)

The only ways to avoid oxidation is:

1. Restrict access to Oxygen
2. Put enough antioxidant into the solution (Vitamin C)


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Re: The Official Woodlovers Thread [Re: Adas]
    #26831308 - 07/18/20 06:22 PM (22 days, 21 hours ago)

3. Create a stable salt that doesn't oxidise readily . . .

If such a salt exists. . .


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OnlineAdas
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Re: The Official Woodlovers Thread [Re: Bobbit]
    #26831917 - 07/19/20 04:23 AM (22 days, 11 hours ago)

I don't think the salt form matters when it comes to oxidation. I highly doubt that such a salt exists (unless the acid has some reducing power, like ascorbic acid).


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