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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,344
Loc: North/Western WA
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Quote:
TheMadHatter420 said: So I want to make some softer agar to aid in germination some wood lover spores. I normally do 2g/100 and was thinking about 1.5/100. Now thing is I am not sure just what would be to soft. Anyone got any advise here. I really want the subaeruginosa to germinate as I was only gifted a small piece of a print. I got a couple prints of the other to keep trying with.
use the recipe on pasty's plate tek but instead of the potato use grain soak water for your water measurement. Don't forget the drop of karo.
Nice to see you Nobler
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wolfedawwg
Veteran Lib Hunter & Gatherer



Registered: 10/27/11
Posts: 893
Loc: Nova Scotia
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Quote:
TheMadHatter420 said: So I want to make some softer agar to aid in germination some wood lover spores. I normally do 2g/100 and was thinking about 1.5/100. Now thing is I am not sure just what would be to soft. Anyone got any advise here. I really want the subaeruginosa to germinate as I was only gifted a small piece of a print. I got a couple prints of the other to keep trying with.
I used up the last of my agar the other night. I had 1.7 g agar left which I added to 150 ml of grain/wood water and it's borderline. It seems great for germinating so far but it was a disaster doing transfers yesterday. It was way too soft, when I would attempt to do the transfer I couldn't stick it to the receiving plate, it would just push into the agar.
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Easy as Fuck Isopropyl Hash Oil Psilocybe quebecensis   
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: wolfedawwg] 1
#24736212 - 10/25/17 10:18 AM (6 years, 6 months ago) |
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Soluble agar, and soluble liquid culture | Alternative DIY method. Germinates everything in 3-5 days, average, room temp.
Requires aseptic (sterile) working (be clean).
For nutrients provided, see here.
Recipe:
Optional: 1-2g > Calcium carbonate powder
2-3g > Dextrose monohydrate (brewers sugar, see here) 0.1g > Miracle-Gro, or other, soluble nutrients 0.1g > Yeast nutrient (or yeast extract)
100g > Boiling hot water 2.8g > Agar powder *

Not ideal for cleaning purposes.
Edited by Ferather (10/25/17 10:35 AM)
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,344
Loc: North/Western WA
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Re: The Official Woodlovers Thread [Re: Ferather] 1
#24736267 - 10/25/17 10:55 AM (6 years, 6 months ago) |
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,307
Loc: Central EU
Last seen: 13 hours, 13 minutes
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Just found a GIGANTIC P. serbica today. Printing in progress. That shit is (almost) as big as Cyanescens.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Working with T-Gel antibacterial agar, and wood pegs:
T-Gel agar uses no added starch or sugar, laccase enzymes and the mycelium produce sugars via oxidization.
Here is oyster, strong enough to "selectively" devour mold, and a bacterial competitor. This is infected WBS (wild bird seed) and T-Gel agar (modified camera settings).

 ---- Also, green mold is a wood loving mycelium, see some pics here, it will therefor germinate on pretty much any substrate supporting germination. In my above pictures, its producing soluble food via enzymes, so not only is it being devoured, but it's also attracting oyster growth. The oyster will produce the same enzymes as the mold (both wood loving, and both are primary decomposers).
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Here are some images of oyster oxidizing T-Gel (extra strong) using laccase.
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Ideal for cleaning lignicolous mycelium. Caution: Can germinate spores.
Risk factor, exposed: Low.
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svamp
Registered: 09/03/15
Posts: 25
Loc: Scandinavia
Last seen: 6 years, 2 months
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Re: The Official Woodlovers Thread [Re: Ferather] 1
#24736329 - 10/25/17 11:17 AM (6 years, 6 months ago) |
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very nice guys and girls, i am gettig all jaloux of all the myco-prn pictures. never tried cultivating woodlovers
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,307
Loc: Central EU
Last seen: 13 hours, 13 minutes
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Re: The Official Woodlovers Thread [Re: svamp] 3
#24736344 - 10/25/17 11:21 AM (6 years, 6 months ago) |
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Adding some pics of today's Serbicas. The two whitish ones were big, but look at that monster in my hand.

Also, found this six-pack of tumors. You don't see that every day!
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: svamp] 3
#24736359 - 10/25/17 11:25 AM (6 years, 6 months ago) |
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Quote:
svamp said: ..never tried cultivating woodlovers
They come armed with laccase, something like cubensis fails to do either well or at all. Lignicolous mycelium will self provide sugars to improve cellulose decay.
Not as fast as absorbing sugars directly, but much safer.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: Ferather] 6
#24736481 - 10/25/17 12:18 PM (6 years, 6 months ago) |
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Wood, untreated is about 50% carbon (from cellulose, lignin, other), and 0.1% nitrogen: [500:1] = bad yield, weak-slow growth. All wood loving types benefit from the addition of missing, low nutrients, wood has a very low nitrogen ratio (cold).
Many species can be picky about what proteins they detect, sometimes in specific format. Oysters will trap nematodes, and digest them as a source of protein (nitrogen).
The most universal format, is soluble ammoniacal or ureic nitrogen. Dung contains both ammoniacal and ureic nitrogen.

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Manure (decayed foods), note the three main nutrients, contains both ammoniacal and ureic nitrogen, high water content.

Alternative sources, note matching nutrients, plus many more:

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Straw analysis:

Wood analysis:

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Paper pellets:

Basic nutrient list, plant fiber based: Carbon, Potassium, Phosphorus, Magnesium, Chromium, Copper, Iron, Manganese, Silicon.
If you don't add fast carbon (starch, sugar, lignin, other solubles), you don't get contamination germinating. Using spawn, you have two potential options, enriched and 10%+ spawn, or 50/50 unchanged.
Convert spawn as, roughly: -55.55% carbs = carbon, -84% proteins = nitrogen. Convert paper as, roughly: -64.55% dry = carbon, -99.8% dry = nitrogen.
20:1 for best yield, 40-60:1 for faster colonization.
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If that fails, seek bacteria, or specific woods.
Also wood is acidic, check the best pH.

For live oyster mycelium, pH 7-8 produced the most vigorous growth. Calcium carbonate, powdered, can be used to increase pH.
Most acidic materials decay above pH 6.5-7.
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Multiple data sites stated an increase in laccase production with the addition of wheat bran.
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Rough carbon to nitrogen guide:
Wood products: Contain about 50% carbon, 0.1% nitrogen, this is a carbon to nitrogen ratio of 500:1 (0.1 x 10, until 1%, 50 x 10 = 500). Paper products: Contain about 40% carbon, 0.2% nitrogen, this is a carbon to nitrogen ratio of 200:1 (0.2 x 5, until 1%, 40 x 5 = 200).
Wheat bran, example:
Here is wheat bran, with total nitrogen of 2.560g per 100g. Wood has about 0.1g of nitrogen per 100g.
100g wood + 100g bran = 200g, 0.1 + 2.560 = 2.660, 2.660 / 2.00 = 1.33% nitrogen. 100g wood + 75g bran = 175g, 0.1 + 1.920 = 2.020, 2.020 / 1.75 = 1.15% nitrogen. 100g wood + 50g bran = 150g, 0.1 + 1.280 = 1.380, 1.380 / 1.50 = 0.92% nitrogen. 100g wood + 25g bran = 125g, 0.1 + 0.640 = 0.740, 0.740 / 1.25 = 0.59% nitrogen.
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Some species-strains may like nitrogen rich leaves with their wood. An example would be tea leaves, nice and rich in nutrients.
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Wood ash, composition data here.
Edited by Ferather (11/01/17 10:43 AM)
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Mike O Voidenski
Undomesticated Rockoholic



Registered: 09/04/17
Posts: 295
Loc: North America
Last seen: 6 years, 5 months
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Re: The Official Woodlovers Thread [Re: Ferather] 3
#24737011 - 10/25/17 03:56 PM (6 years, 6 months ago) |
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Bad ass! Maybe this information can be used to help make better woodlover cakes.
-------------------- There are three distinct realities occurring simultaneously. The first involves a single point or object. The second involves that point/object's relationship with other points/objects. The third involves their relationships in motion.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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I cant see why not, to be fair, gourmet wood loving, is wood loving. Same basic settings, some changes here and there.
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 7 months
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Re: The Official Woodlovers Thread [Re: Ferather] 3
#24737192 - 10/25/17 05:30 PM (6 years, 6 months ago) |
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Thanks for that post Ferather... it's great to have all that info in one place. Will definitely reference that again!
Here's some Az ripping through a random chip blend. Standard grain spawn > 2gal > 5gal. The recovery/leap-off is fantastic.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: Primal Call]
#24737202 - 10/25/17 05:34 PM (6 years, 6 months ago) |
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Nice ratio of spawn to substrate, looks great, what grain was it?
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Primal Call
Earth Mage



Registered: 09/05/10
Posts: 2,766
Loc: Here until here
Last seen: 4 years, 7 months
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Re: The Official Woodlovers Thread [Re: Ferather]
#24737223 - 10/25/17 05:42 PM (6 years, 6 months ago) |
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Oh... to clarify: I used a few pints (3 or 4) of spawn to the 2gal. That was done in a month or so, then the 2 went to the 5.
Right now I'm using hard red spring wheat from Great River Organic Milling.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,307
Loc: Central EU
Last seen: 13 hours, 13 minutes
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Re: The Official Woodlovers Thread [Re: Primal Call] 7
#24738445 - 10/26/17 07:09 AM (6 years, 6 months ago) |
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You know the big Serbica I found yesterday?
Forget about that... Bumped into a new secret place!

Nearly shat my pants that's for sure. They apparently love conifer branches.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: Adas]
#24738467 - 10/26/17 07:28 AM (6 years, 6 months ago) |
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Quote:
Primal Call said: Oh... to clarify: I used a few pints (3 or 4) of spawn to the 2gal. That was done in a month or so, then the 2 went to the 5.
Right now I'm using hard red spring wheat from Great River Organic Milling.
Oh right, I thought that was 2 gal dry spawn, to 5 gal dry wood (28.5% spawn).
Quote:
Adas said: You know the big Serbica I found yesterday?
Forget about that... Bumped into a new secret place!

Nearly shat my pants that's for sure. They apparently love conifer branches.
That's a very nice bunch you have there. Conifer, interesting.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,307
Loc: Central EU
Last seen: 13 hours, 13 minutes
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Re: The Official Woodlovers Thread [Re: Ferather] 1
#24738477 - 10/26/17 07:43 AM (6 years, 6 months ago) |
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Fuck, I put them into the oven to dry at 50°C and they are bluing and releasing tons of water and sticking to the paper. 
I'm NEVER drying them like this again. I hope they didn't lose too much potency. But the bluing tells me they did..
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: Adas]
#24738493 - 10/26/17 07:58 AM (6 years, 6 months ago) |
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Shame you cant dehydrate them in a CO2 or no air environment.
I've considered storing them in carbonated water. Or even something like fizzy lemonade.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,307
Loc: Central EU
Last seen: 13 hours, 13 minutes
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Re: The Official Woodlovers Thread [Re: Ferather] 1
#24738514 - 10/26/17 08:08 AM (6 years, 6 months ago) |
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Oven is good, but you need to open-air dry them a bit first. Otherwise they'll become a sticky wet mess.
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