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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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The Ps Cyan print you sent me had no problem germinating on LME, it took 4-5 days and still looks visibly clean
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 17 days
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I never had problems getting them to germ on MEA or straight grain. Maybe make up a few no pours with some grain and see if that helps. Just transfer a grain to an agar plate to clean it up from there.
I've heard you can use the water from soaking or boiling woodchips in agar and lc to help for later vigor but I don't think I read it's helps with germinating but that would make sense.
edit... damn boys you beat me to it
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Edited by tombosley8 (10/22/17 11:41 AM)
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,942
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Re: The Official Woodlovers Thread [Re: tombosley8]
#24729240 - 10/22/17 11:41 AM (6 years, 6 months ago) |
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OK, I may just need to give them a few more days, or maybe the no pour plates need a little softer agar with a little more moisture.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 17 days
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yeah slightly softer agar has always helped me with germinating.
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
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Quote:
TheMadHatter420 said: Boys I am having some issues getting wood lover spores to germinate, I have them on grain broth agar right now, the cubes and gourmet cultures love it. Should I try soaking some wood and using that broth with ME to make agar, or what is the go to method to get wood lover spores to germinate?
If you're having trouble getting the active woodlovers to germinate, try germinating them in 60F.
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,942
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Re: The Official Woodlovers Thread [Re: hamloaf]
#24729421 - 10/22/17 01:07 PM (6 years, 6 months ago) |
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Well I just cleaned up some dishes from the same agar batch and honestly, that shit is a bit hard and all I use is 2g/100ml so I may try softer agar. My work area is in mid to upper 60's right now. I tried throwing a plate of ovoid in the fridge, in a zip lock bag, and that didn't seem to help. I may need more moisture as well as the extra moisture is now gone from those plates. Fucked up part is I did germinate cyan spores before.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 17 days
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yea slightly lower temps could help a lot as ham said and also try a couple brf plates. They'll germinate damn near anything for me.
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Edited by tombosley8 (10/22/17 01:42 PM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Re: The Official Woodlovers Thread [Re: hamloaf]
#24729494 - 10/22/17 01:56 PM (6 years, 6 months ago) |
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Quote:
hamloaf said: If you're having trouble getting the active woodlovers to germinate, try germinating them in 60F.
Tested and I agree, general idea: Germinate at fruiting temperatures, if below room temperature. You should go for the higher end of the tolerated fruiting temperature, for better speed.
Example: Fruiting temperature range 10-16°C, try germination at 16°C.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 17 days
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Re: The Official Woodlovers Thread [Re: Ferather] 1
#24729506 - 10/22/17 02:01 PM (6 years, 6 months ago) |
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idk fruiting temps are average of 40-50 degrees I believe.
I found colonization and germinating worked great around 70 degrees and slightly increased germinating at about 60 degrees.
I never tried germinating at anything lower than 50 so I can't say for sure but I didn't notice much benefit and very possibly the opposite from that low of temps
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,942
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Re: The Official Woodlovers Thread [Re: Ferather]
#24729508 - 10/22/17 02:02 PM (6 years, 6 months ago) |
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K, I will try germinating them in a cooler part of the basement. I need to hang a heat lamp, in opposite corner, in the area I work, cause its getting a little cold for my cubes, growth is slowing down as the temps drop.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 6 months
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Indeed, the lower the temperature the slower the growth, however the lower temp is only needed to germinate. One you have visible growth, you can place into normal working conditions, speed should improve.
You no longer need the lower temperature, so even spawn can be made at room temps. Until fruiting conditions are required, then reduce temperature to suit.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 11 months, 17 days
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Re: The Official Woodlovers Thread [Re: Ferather]
#24729533 - 10/22/17 02:18 PM (6 years, 6 months ago) |
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ok kool thanks.
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,344
Loc: North/Western WA
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Re: The Official Woodlovers Thread [Re: tombosley8]
#24729692 - 10/22/17 03:55 PM (6 years, 6 months ago) |
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Give them a few extra days to germ.
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
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Re: The Official Woodlovers Thread [Re: Ferather] 1
#24729744 - 10/22/17 04:16 PM (6 years, 6 months ago) |
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Quote:
Ferather said:
Quote:
hamloaf said: If you're having trouble getting the active woodlovers to germinate, try germinating them in 60F.
Tested and I agree, general idea: Germinate at fruiting temperatures, if below room temperature. You should go for the higher end of the tolerated fruiting temperature, for better speed.
Example: Fruiting temperature range 10-16°C, try germination at 16°C.
Yea, my ovoids, and azurescens wouldn't crack after several attempts until the spore plates were sustained at 60-65F.
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Re: The Official Woodlovers Thread [Re: hamloaf] 7
#24730322 - 10/22/17 08:11 PM (6 years, 6 months ago) |
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TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,942
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Re: The Official Woodlovers Thread [Re: liloldme]
#24730340 - 10/22/17 08:18 PM (6 years, 6 months ago) |
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Just fucking beautiful!!!! I take it that is the patch you started last year.
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TheDuder
Mushroom Hunter



Registered: 11/07/16
Posts: 2,544
Loc: PNW
Last seen: 1 year, 4 months
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Damn nice one dude! Checked one the azure bed I laid a few weeks ago, the grass seed I put down sprouted. I hope that helps keep moisture in. Got some ground cover strawberry and rubus growing too.
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|-------------------[Ps. Azurescens]------------------------------------------[Ps. Semilanceata]--------------------------------------------[Ps. Allenii]------------------------|
|--------------[Ps. Ovoideocystidiata]------------------------------------------[Ps. Stuntzii]--------------------------------------------[Ps. Baeocystis]----------------------|
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NothingsChanged
Striving for Excellence



Registered: 05/28/11
Posts: 10,344
Loc: North/Western WA
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liloldme
( ͝° ͜ʖ͡°)つ=D



Registered: 05/15/04
Posts: 5,087
Loc: Zone 8
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Quote:
TheMadHatter420 said: Just fucking beautiful!!!! I take it that is the patch you started last year.
One of several
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 21,756
Loc: ation undisclosed.
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Re: The Official Woodlovers Thread [Re: liloldme]
#24730934 - 10/23/17 07:20 AM (6 years, 6 months ago) |
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That's dope, lilme. Woot woot woot!
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