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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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to Land Trout & others:
i've been thinking it makes sense to not isolate on agar for outdoor patches. I've got 4 'strains' from spore germination on agar, and i've been doing 2-3 transfers on each , trying to not eliminate sectoring that may be occuring.
one of the plates just comes out round, growing vigorously and symmetrical without being transferred much. I Just now put that to grain. The other 3 strains are on their 3rd run on agar.
I was thinking of putting each strain to grain then testing out the patches, but it sounds like I should just mix the cultures. Should that be done sooner rather than later? Should I throw a wedge of all 4 strains in each grain jar, seems a little easier to mix grains later on. Or even bags of sawdust spawn. (is Grain spawn to sawdust spawn ok? or I could add alder shavings (from a planer) or possibly wood chips
Edited by Generic (01/09/24 05:22 PM)
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Re: The Official Woodlovers Thread [Re: ghiajake]
#28618804 - 01/12/24 03:06 PM (15 days, 8 hours ago) |
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Quote:
ghiajake said: Think generationally. Indoor culturing is faster and more controlled, but outdoor culturing is a lot slower since it's on an annular cycle and subject to the elements. If you sector before fruiting, you'll have no idea if you eliminated the genetics that allow it to better naturalize to your specific microclimate.
That's how I'm thinking... I like what you've done, by selecting for traits and cloning and all, If we germinate spores then its really breeding for our conditions/bioregion.
So how would we best start from spores with as many strains as possible while still ensuring clean spawn? I figured to select at least 4 strains; it seemed like a lot of agar work but not too much. Any other ideas?
I also figured to let the original germination plate grow for a while for them to have time to mate, does this seem right?
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Re: The Official Woodlovers Thread [Re: ghiajake]
#28619111 - 01/12/24 07:39 PM (15 days, 3 hours ago) |
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Quote:
ghiajake said:
As to your original plate. Yeah... Leave it alone until it's fully colonized, then inoculate a master jar or two of grain spawn with it and G2G for spawn expansion from there.
If you're asking me how to "start from spores with as many strains as possible while still ensuring clean spawn", that tells me you don't have much experience with lab work and should NOT dive into large agar projects. Get your lab work process down tight first, and you won't have to ask how to make "clean spawn".
Sure, my terminology's off, but with the idea of going from spores to agar, I saw one germ plate you mentioned that could just be sent to grain as is, and I was thinking you would surely want to do at least one transfer unless you get good at making good spore prints and streaks. For me, I had to transfer away from bacteria.
I'll just combine everything when spawning
But when we germinate spores, and see mycelium from those points, are those not unique strains? There's probably many definitions of 'strain'. I like how in the plant world there are 'varieties' and there are 'cultivars', or cultivated/traded varieties. Can you name some strains of woodlovers do you know of that are selected for a certain trait as you say?
Edited by Generic (01/12/24 07:41 PM)
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Re: The Official Woodlovers Thread [Re: rhizoRider]
#28625936 - 01/18/24 01:36 PM (9 days, 9 hours ago) |
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I would like some opinions as to what wood to use for cyanescens...
I have Doug Fir (sawdust) Fuel Pellets, which has seemed to colonize fine with a quasi-pasteurization (boiling water poured into bags inside a cooler, allowed to sit at 160-170 for 2 hours) with Reishi and Oyster.
I also am getting Alder planer shavings, so fluffy, not sawdust, but good alder. I figure I'd have to sterilize this, so would a mix of Doug Fir sawdust and alder be very good?
It would be easier to quasi-pasteurize DFFP and colonize in XL filter patch bags, but these could also be sterilized if the Alder is worth it.
Then after there are spawn bags, I'm curious about how people like to do it in totes or contractor bags or some other way to get more wood to colonize inside before spawning outdoors in the spring.
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Re: The Official Woodlovers Thread [Re: Land Trout]
#28626080 - 01/18/24 03:49 PM (9 days, 7 hours ago) |
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OK woodchips are best, got it.
I was assuming it would be grain to sawdust spawn to woodchips... thank you all.
So would it be best to go from grain spawn to unsterilized wood chips, no sawdust involved? t I'm still curious about the colonization vessels at that point. I have lots of XL filter patch bags and plastic totes I could use. But unsterilized wood chips inside seems contaminant prone. I probably need to get over my affinity for sterilization for this; So after grain spawn, nothing should be sterilized or pasteurized is the consensus?
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Thanks for pointing out that context is key and when it would be appropriate to use sawdust spawn.
When the trees are dormant is the best time to get chips in general, before their energy goes into their foliage in spring and then there gets to be leaves in the chips.
I like hearing that bit about cedar, I might mix some in, it makes sense to me.
And I love hearing that you hand-chip the wood, I think I'll try that more in this context.
Time to find or make chips
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Re: The Official Woodlovers Thread [Re: JW123] 2
#28636160 - 01/26/24 08:23 PM (1 day, 2 hours ago) |
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Does anyone have any ideas on what these look like?   
Also I've been curious about soaking chips; I have a 10 foot diameter, 1000 gallon open-topped water tank that's maybe 3 feet tall. It works great for soaking chips, and I can pitchfork off the top to get the good big chunks, while some finer stuff sinks. It's a lot of work, to move wet wood chips so many times. I'm thinking of making it so my truck can drive to that tank. That's what I've done before for outdoor Oyster patches on wood chips, but I've always questioned the soaking part. Soaking chips seems to be recommended all the time for gourmet woodlovers. Maybe that's becaues sawdust spawn is usually recommended before that...
As mentioned earlier perhaps it's harder for mycelium to run from sawdust spawn onto chips than to sterilize chips and have those colonize indoors, then use the chips as spawn for the outdoor chips.
I would lean towards soaking with a pile that's mostly hardwood but has some dead oak tree(s) pieces in there that seem dry, just to hydrate things. If it were all freshly cut living wood I would feel better about not soaking
I'm interested more in the option of fermenting... How many people currently soak chips for so long they ferment? is it an anaerobic fermentation thing that kills off the aerobic fungi in the chips, perhaps?
who soaks their wood chips?
who never does?
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 5 hours, 45 minutes
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Re: The Official Woodlovers Thread [Re: Land Trout]
#28637066 - 01/27/24 03:27 PM (7 hours, 45 minutes ago) |
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So I got a dump load of wood chips, I removed parts that didn't look ideal, and spread the pile out so that it's A foot and a half tall.
Big piles create heat and competitors, So i always spread them thinner. Now the rain is soaking in to the top... inch.
I'm tempted to spread it thinner to soak up more rain.
But then I think, why don't i just put some of the wood chips in their final place already. When it comes time to spawn outdoors, would it be too hard to mix in spawn with the wood chips if at that time more wood chips were added?
Also, Has anyone ever put spawn directly in a wood chipper?
Edited by Generic (01/27/24 03:27 PM)
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