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OfflineMycoMechanic
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spore syringe to agar - how long to wait?
    #24662441 - 09/26/17 09:34 PM (3 years, 7 months ago)

Dripped from known good spore syringes to some agar plates I poured. A week later nothing. Is that normal?

Agar is based on grain cooking water, 400ml of that with 11g I think of Agar powder - can't find the recipe I used but maybe it is too hard?


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OfflineEnkidu
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Re: spore syringe to agar - how long to wait? [Re: MycoMechanic]
    #24662639 - 09/26/17 10:41 PM (3 years, 7 months ago)

A week seems like it might not be too long. I just recently went from MS to Agar but unfortunately I can't remember how long it took.

I was impatient and asking the same question until it popped up.

Longer than a week might not be out of the question at all but honestly I can't remember and my experience is super limited >.<

How many do you have?


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OfflineMycoMechanic
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Re: spore syringe to agar - how long to wait? [Re: Enkidu]
    #24662691 - 09/26/17 10:56 PM (3 years, 7 months ago)

Just two - one for each type.


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OfflineDrgreenthumbMN
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Re: spore syringe to agar - how long to wait? [Re: MycoMechanic]
    #24662888 - 09/27/17 12:23 AM (3 years, 7 months ago)

Hey man Im new to mycology I inoculated agar on 9/21 and this is my growth today. I have one dish with contam but the rest look fine



Edited by DrgreenthumbMN (09/27/17 12:24 AM)


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OfflineMycoMechanic
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Re: spore syringe to agar - how long to wait? [Re: DrgreenthumbMN]
    #24663286 - 09/27/17 06:29 AM (3 years, 7 months ago)

awesome!

I'm leaving today on vacation for a week so not much i can do about it either way.


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Invisibleherrenvolk


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Re: spore syringe to agar - how long to wait? [Re: MycoMechanic] * 1
    #24663299 - 09/27/17 06:37 AM (3 years, 7 months ago)

Spores typically take 1-2 weeks to germinate. But I think your agar recipe is a bit too firm. You could probably reduce that to 6-7g agar for better results. But there's still time, give it another week. Especially if it's an old spore syringe.


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OfflineEnkidu
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Re: spore syringe to agar - how long to wait? [Re: herrenvolk]
    #24663440 - 09/27/17 09:04 AM (3 years, 7 months ago)

Make some more if you can before you leave and inocc with a drop of MS.

I would make like 6. Maybe more >.<

I guess idk how many plates you have though or agar.

More is better though.

Better chances.

Good luck


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InvisiblebodhisattaM
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Re: spore syringe to agar - how long to wait? [Re: MycoMechanic]
    #24663590 - 09/27/17 10:50 AM (3 years, 7 months ago)

Somewhere between 2 and 30 days usually


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InvisibleGluckon
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Re: spore syringe to agar - how long to wait? [Re: MycoMechanic]
    #24663628 - 09/27/17 11:06 AM (3 years, 7 months ago)

I inoculated some agar recently and nine days from inoculation I was able to make transfers.


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OfflineVariegated
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Re: spore syringe to agar - how long to wait? [Re: Gluckon]
    #24663643 - 09/27/17 11:10 AM (3 years, 7 months ago)

Put some on agar for the first time ms to agar. 8 days and i could transfer.


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OfflineMycoMechanic
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Re: spore syringe to agar - how long to wait? [Re: Variegated]
    #24663723 - 09/27/17 11:37 AM (3 years, 7 months ago)

Thanks all, going to drop some more today before I go. hoping that gets me somewhere


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OfflineMycoMechanic
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Re: spore syringe to agar - how long to wait? [Re: MycoMechanic]
    #24664264 - 09/27/17 03:15 PM (3 years, 7 months ago)

Well the good news is I know my Agar was good, the bad news is I didn't store them properly and most of my plates were contaminated. I had them in a plastic bag and the fell sideways in my box letting the lids on several open.

Anyway, 2 are still good and I hit them with a squirt from my syringe again.


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