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Offlinend595
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Contam?? PIC
    #2458461 - 03/21/04 10:16 PM (12 years, 8 months ago)

I was wondering if these cakes are contaminated. There has been some condensation in the cakes which was delt with by removing the tape from the holes but it did not help the build up and was wondering if these cakes are contaminated now.




Edited by nd595 (03/22/04 07:37 PM)


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OfflineShroomInhaler
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Registered: 03/04/04
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Re: Contam?? PIC [Re: nd595]
    #2458974 - 03/22/04 12:38 AM (12 years, 8 months ago)

The pic is pretty small so I cant tell. What was your substrate?


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OfflineSuntzu
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Re: Contam?? PIC [Re: nd595]
    #2459022 - 03/22/04 12:57 AM (12 years, 8 months ago)

Can you get a closer shot?

The whiteness is a good sign. . .


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Offlinend595
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Re: Contam?? PIC [Re: Suntzu]
    #2459538 - 03/22/04 08:15 AM (12 years, 8 months ago)

sorry about the bad pic but it would't let me upload the one that was closer, didn't realize you only got 1 every 24hours. SO when i can put another one up i will. And i guess i can describe it, its ona PF tek cakes, and it just showed up yesterday, its a brown rust color spot, 2 of them on 2 different cakes, they are both very small. The have been isolated from the other cakes.


--------------------
"He is one of those people who would be enormously improved by death."
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Everything i say, do, or post is a lie Don't believe a word i say


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OfflineReTrIbUtIoN
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Re: Contam?? PIC [Re: nd595]
    #2459794 - 03/22/04 11:58 AM (12 years, 8 months ago)

is it yellowish? most likely mycelium piss from what i can see. Are the jars 100%? if so remove the cake take a closer look at the spot and if needed cut it out and sterilize the spot with rubbing alcohol.


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Offlinend595
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Re: Contam?? PIC [Re: ReTrIbUtIoN]
    #2460260 - 03/22/04 03:17 PM (12 years, 8 months ago)

The color is like a rust brown, the jars were turned over and the cake slipped down a little bit and it let the brown color stuff stuck to the jar, and a hole were the mycelium was. It is on two other jars and has not grown at all in a little over a day. THe cakes that it is is on are all about 90% colonized. I checked on some petri dishes and agar and i noticed on one cake it had a similar growth, just one small spot with the rust brown color to it about the size of a pin head.


Edited by nd595 (03/22/04 07:36 PM)


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Offlinend595
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Re: Contam?? PIC [Re: nd595]
    #2461654 - 03/22/04 10:27 PM (12 years, 8 months ago)

heres the new pic, sorry its the best i could do up close, my camera ain?t to great.



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OfflinebioTek
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Re: Contam?? PIC [Re: nd595]
    #2461897 - 03/22/04 11:31 PM (12 years, 8 months ago)

looks like it's more on the glass than on the myc. maybe it's substrate sludge that wasn't colonized?


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Offlinend595
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Re: Contam?? PIC [Re: bioTek]
    #2463085 - 03/23/04 08:34 AM (12 years, 8 months ago)

It is on the class right now, i flipped the jars over and it slid down some leaving that still stuck to the glass and an empty spot on the cake where that once was.


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OfflineSuntzu
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Re: Contam?? PIC [Re: nd595]
    #2463186 - 03/23/04 10:09 AM (12 years, 8 months ago)

It's still tough to tell, of course. Personally, I'd give it a little time just to see if the brown gets any bigger or more slimy. It looks like it could be uncolonized substrate sludge, as it is only at the bottom where more 'sludgy' stuff would settle. I think the odds are good that it's fine, but give it another couple of days. If it is a minor bacterial growth, upon birthing it will likely disappear.


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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