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oontribe

Registered: 01/14/15
Posts: 3,570

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: Panarchist]
#24423523 - 06/21/17 11:01 AM (6 years, 9 months ago)

:takingnotes:

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c10h12n2o
serial dilutor

Registered: 01/21/15
Posts: 3,200
Loc: the abyss

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: oontribe]
#24424207 - 06/21/17 03:41 PM (6 years, 9 months ago)

thanks guys :smile:

:smile:

im a bit behind on all my chores (working with waaaaay too many species , not to mention races and cultures lol), but i have a nice update for this coming as soon as i get the chance. gonna add lots more pics of examples, distinctions between SAB and FH techniques, cloning, maybe some video demonstrating how to execute particular steps, etc...

im spawning an assload of reishi, king oysters, and cord m, testing a bunch of different substrates and preps

let me know if yall have any questions about anything, want clarification on something, or want to request that i cover a specific topic in subsequent updates :wink:

:wink:

--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide

"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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ManifoldPrime
Per Ardua Ad Astra

Registered: 03/16/17
Posts: 1,313
Loc: South Africa

Last seen: 1 year, 3 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: c10h12n2o]
#24425993 - 06/22/17 07:50 AM (6 years, 9 months ago)

You should do a journal / log of your grows, I really enjoy reading through grow logs to pull cool ideas and whatnot. And you have some interesting varieties going there (What is Cord M?)

--------------------
My Magnum Opus:Thai Variety Comparison
Please correct me if I say something wrong!
| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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foragedfungus

Registered: 09/30/13
Posts: 1,867
Loc: out there

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: ManifoldPrime]
#24426013 - 06/22/17 08:01 AM (6 years, 9 months ago)

Cord m = Cordyceps militaris.

http://www.mushroomexpert.com/cordyceps_militaris.html

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ManifoldPrime
Per Ardua Ad Astra

Registered: 03/16/17
Posts: 1,313
Loc: South Africa

Last seen: 1 year, 3 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: foragedfungus]
#24426017 - 06/22/17 08:05 AM (6 years, 9 months ago)

Oh the zombie shroom, lmao I posted that in the 100 facts thread. I should memorise latin names better :facepalm:

:facepalm:

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NattyDR

Registered: 05/27/17
Posts: 97
Last seen: 1 year, 7 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: ManifoldPrime]
#24440018 - 06/27/17 05:03 PM (6 years, 9 months ago)

Wow, this is incredibly helpful, thank you. I'm just dipping my toes into agar and will be referring back to your guide regularly!

:bow2:

:bow2:

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jkz
Padawan

Registered: 06/16/13
Posts: 1,910
Loc: Coruscant

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: NattyDR]
#24440107 - 06/27/17 05:32 PM (6 years, 9 months ago)

I reference this once a day at least lol

--------------------
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c10h12n2o
serial dilutor

Registered: 01/21/15
Posts: 3,200
Loc: the abyss

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: jkz]
#24440193 - 06/27/17 06:00 PM (6 years, 9 months ago)

thanks guys :smile:

:smile:

im glad yall find it useful :smile:

:smile:

there is a lot of stuff in there that i REALLY wish had been made clear to me when i first started. im a critical thinker, so i take everything apart and ask a million dumb questions in my head about everything, and that can be really overwhelming when you first start agar: there are just so many applications, so many factors involved. some of the things that took me a while to figure out could benefit a newbie from day 1

i have some planned updates i want to add, including some videos (as soon as my camera gets back from the shop), with lots of distinctions between SAB and FH technique, and more on streaking techniques, but ive been playing catchup with all my projects... recently started doing plant tissue culture and good god that shit is complicated... lots of overlap though, i really look forward to learning more about it and applying some of the techniques to our hobby

yall are always welcome to bump this thread with questions, requests, clarifications, racism, pornography, flames, idk :smile:

:smile:

(though mods might)

this is a work in progress. id like to keep it somewhat current with my techniques, because i am always trying and testing new things i read in lab manuals, textbooks, and papers, and will be happy to share anything that others might find useful. i love the thought of newbies getting to start with all the info i wish i had when i started :smile:

:smile:

--------------------

C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide

"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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bill bixby
Stranger

Registered: 06/09/17
Posts: 36
Last seen: 4 years, 6 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: c10h12n2o]
#24461479 - 07/06/17 09:18 AM (6 years, 9 months ago)

:takingnotes:

cheers lad

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Shroomterd
42

Registered: 06/28/17
Posts: 220
Loc: washington
Last seen: 2 years, 8 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: bill bixby]
#24499787 - 07/21/17 09:42 PM (6 years, 8 months ago)

This is awsome definitely moving on to agar next it's insane how these teks have helped me progress in my mycology thank you for your time sir..

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wtfcrazymofo
foil hater

Registered: 07/26/15
Posts: 1,220
Loc: Colonial alley
Last seen: 4 days, 10 hours

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: mushboy]
#24533743 - 08/05/17 09:51 PM (6 years, 8 months ago)

Nice words and pictures in great order. :rockon:

:rockon:

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If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922

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lovelaughlibs
Dopamine Slave

Registered: 10/14/15
Posts: 1,811
Loc: England

Last seen: 6 years, 2 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: wtfcrazymofo]
#24573576 - 08/23/17 05:07 AM (6 years, 7 months ago)

Clearly a lot of effort went into this, would be 5 shrooms rating if I hadn't already done so!

--------------------
Ask and ye shall receive; Seek and ye shall find.

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BreakTheSOB
Stranger

Registered: 07/09/17
Posts: 162
Last seen: 6 years, 5 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: lovelaughlibs]
#24573671 - 08/23/17 06:40 AM (6 years, 7 months ago)

what brand of face shield, and what brand on the reusable black gloves?

--------------------
"A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle

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optic1
Dr. Phil Atio

Registered: 08/18/17
Posts: 31
Loc: WA
Last seen: 6 years, 6 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: c10h12n2o]
#24581368 - 08/26/17 02:09 PM (6 years, 7 months ago)

I spent the time to read this, great post.
Can't believe the time that was spent just to put this together.
Great job C10, thanks for the help.

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Pipefitter537
You're Not Yelping

Registered: 11/25/16
Posts: 715
Loc: SodoSopa

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: optic1]
#24581384 - 08/26/17 02:24 PM (6 years, 7 months ago)

Where the fuck u at c10? Hope everything is ok.

--------------------
"You should never underestimate the predictability of stupidity"-Bullet Tooth Tony

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Sphink
Stranger

Registered: 08/09/17
Posts: 21
Last seen: 3 years, 2 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: Pipefitter537]
#24586423 - 08/28/17 08:32 PM (6 years, 7 months ago)

Excellent write up, I'm just getting started and have been doing a ton of research. I really picked up a ton of information from this post.

I like the information about serial dilution, that brings a whole new dimension and logical thought process to something as simple as smearing spores on agar.

This is something I can read, and reread, and continue to pick up on little techniques. Very well written and logically thought out. Easy to follow. A lot of information in the comments as well.

When you say you use "full strength" agar in your long term slants, what exactly is your recipe? 20g agar, 30g light malt extract per 1000ml water? Also curious as to what size tubes you personally use.

I would love to read your thoughts on long term storage and how you make and store your slants and prints and keep them viable. The photo album is an excellent idea. I know there are plenty of other teks about it out there (franks). But I'd like to hear the processes and equipment you've settled with, and how you maintain your library. If there is anything you do different. Or any tips or tricks you have on the subject from your experience. (C10's storage guide?) Lol

Thanks again, keep up the exceptional work!

Edited by Sphink (08/28/17 09:00 PM)

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ManifoldPrime
Per Ardua Ad Astra

Registered: 03/16/17
Posts: 1,313
Loc: South Africa

Last seen: 1 year, 3 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: Sphink]
#24586945 - 08/29/17 01:36 AM (6 years, 7 months ago)

Quote:

20g agar, 30g light malt extract per 1000ml water

Thats a little strong, usually youd use 20g malt and around 2-4 g dextrose. It would work, but generally speaking you want your nutrient content to be on the low side, you want the myc to work for its dinner and spread out faster :biggrin:

:biggrin:

--------------------
My Magnum Opus:Thai Variety Comparison
Please correct me if I say something wrong!
| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

Extras:

Sphink
Stranger

Registered: 08/09/17
Posts: 21
Last seen: 3 years, 2 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: ManifoldPrime]
#24587481 - 08/29/17 09:30 AM (6 years, 7 months ago)

Quote:

ManifoldPrime said:
Quote:

when you say you use "full strength" agar in your long term slants, what exactly is your recipe? 20g agar, 30g light malt extract per 1000ml water?

Thats a little strong, usually youd use 20g malt and around 2-4 g dextrose. It would work, but generally speaking you want your nutrient content to be on the low side, you want the myc to work for its dinner and spread out faster

Yes, for petri's you want nutrients on the low side to encourage rhizomorphic growth and sectoring. C10 did a good job explaining that in the original post and suggests "half strength" agar. But for master slants you would want the opposite, no?

wouldn't you want it to grub out and stay put to avoid unnecessary cell division? Thus the refrigeration ect...

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mekannic
just dropped 2 hits

Registered: 09/28/14
Posts: 238
Last seen: 1 year, 5 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: c10h12n2o]
#24636430 - 09/16/17 09:03 PM (6 years, 6 months ago)

hey U4EUh, nice writeup, hope all is well, nobody heard from you in a while

put together a couple things from what you condensed, thanks much

again, hope all is well

--------------------
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"the leaf doesn't make it's own decision's. Instead, it is the wind that decides where the leaf shall be, and how long the trip shall take"-m

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Psychedeliciouss
Scrappy CoCo

Registered: 10/18/17
Posts: 110
Last seen: 5 years, 4 months

Re: c10's Agar Guide + Tips & Tricks (post #1000)

[Re: mekannic]
#24831774 - 12/07/17 11:28 AM (6 years, 4 months ago)

hey thanks, great post and nice written. i have a question, if i follow your recipe and also make dishes thin like you it will be possible to let them sit in the fridge for 1 month and later use them with success?

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Pages: < Back | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Next > [ show all ]

Shop:

Buy Bali Kratom Powder

Buy Kratom Extract

Unfolding Nature: Being in the Implicate Order

Liquid Cultures

Isolated Cubensis Liquid Culture For Sale

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Certified Organic All-In-One Grow Bags

Red Vein Kratom

CBD Concentrates

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