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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Contam identification please.
#24574714 - 08/23/17 04:56 PM (6 years, 6 months ago) |
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Whats this contam? Brown color all over mycelium seems to have taken over in 2 days. Also, this jar has been removed from my incubation chamber where i keep my liquid cultures. I have two honey based cultures in the box and it smells like alcohol? Is this normal?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Contam identification please. [Re: Amante]
#24574719 - 08/23/17 05:00 PM (6 years, 6 months ago) |
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Can you make a slide and get an image through a microscope?
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Re: Contam identification please. [Re: bodhisatta]
#24574725 - 08/23/17 05:03 PM (6 years, 6 months ago) |
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If only, then i could just cross refernce and bingo.. was hoping someone has seen a full brown/yellow takeover before and would be able to pass on some knowledge of what it is, maybe how it works and what to watch out for. My others are going good aside from the alcohol smell in my incubation chamber.
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Re: Contam identification please. [Re: Amante]
#24574938 - 08/23/17 06:52 PM (6 years, 6 months ago) |
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Anyone else have a clue? Bump...
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Re: Contam identification please. [Re: Amante]
#24577229 - 08/24/17 04:43 PM (6 years, 6 months ago) |
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Just bumping again. Still curious, would like to just use it outside and see if something fruits but if its something dangerous ill just dump it.
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"Through our eyes, the universe perceiving itself. Through our ears, the universe is listening to its harmonies. We are the witnesses through which the universe becomes conscious of its glory, of its magnificence.” -Alan Watts
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MarilynManson
Psilence


Registered: 03/24/10
Posts: 69
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Re: Contam identification please. [Re: Amante]
#24577238 - 08/24/17 04:46 PM (6 years, 6 months ago) |
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My lc looked similar with to much honey. If u shine ur flash light on it under it you'll see it's pretty much white. However one of mine i believe had trich on it as wellthat smelled like alcohol it seemed like steaming It deformed the lid therefore no seal. Its hard to tell honestly unless you do a test run. Could try agar/isolate/isolate etc if you really need it
Edited by MarilynManson (08/24/17 04:47 PM)
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mynakedrat
The phantom hourglass



Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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That's looks? Myc is good. Water is mostly clear. Test first, on a cake or Small jar. Or a plate
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Just bumping again. Still curious, would like to just use it outside and see if something fruits but if its something dangerous ill just dump it.Quote:
MarilynManson said: My lc looked similar with to much honey. If u shine ur flash light on it under it you'll see it's pretty much white. However one of mine i believe had trich on it as wellthat smelled like alcohol it seemed like steaming It deformed the lid therefore no seal. Its hard to tell honestly unless you do a test run. Could try agar/isolate/isolate etc if you really need it
I will check with a l.e.d to see if it looks white. Also whats your recomended testing method. Is agar and isolate two different methods or a series of steps. I started from a spore print. They were some feild shrooms but i wanted to do everytjing myself. Its a pride thing. I know its very likely it can have a contam. If it looks good to yall though then thats great news. Ill read up on testing, care to link me to a good thread? Thanks in advance.
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"Through our eyes, the universe perceiving itself. Through our ears, the universe is listening to its harmonies. We are the witnesses through which the universe becomes conscious of its glory, of its magnificence.” -Alan Watts
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Cameron1996
Stranger
Registered: 11/28/15
Posts: 384
Last seen: 1 year, 10 months
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Re: Contam identification please. [Re: Amante]
#24577328 - 08/24/17 05:25 PM (6 years, 6 months ago) |
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Metabolites possibly or carmalization? Maybe bacteria. Only way to test is to inoculate and perform a smell check
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Re: Contam identification please. [Re: Cameron1996]
#24578531 - 08/25/17 08:51 AM (6 years, 6 months ago) |
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What exactly am i smelling for. A sourness right?
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"Through our eyes, the universe perceiving itself. Through our ears, the universe is listening to its harmonies. We are the witnesses through which the universe becomes conscious of its glory, of its magnificence.” -Alan Watts
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Contam identification please. [Re: Amante]
#24578535 - 08/25/17 08:53 AM (6 years, 6 months ago) |
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Smell helps but a lot of contamination including bacterial wont make a smell so its not a end all be all test
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Re: Contam identification please. [Re: bodhisatta]
#24578551 - 08/25/17 08:59 AM (6 years, 6 months ago) |
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Okay i will inoculate a pint jar and see where it takes off. I dont wanna waste a bunch of grain using the whole jar and i dont have self healing injection ports so what would be the best process. Feel free to link me somewhere. I do read but its hard to pick out the viable information from the garbled nonsense.
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"Through our eyes, the universe perceiving itself. Through our ears, the universe is listening to its harmonies. We are the witnesses through which the universe becomes conscious of its glory, of its magnificence.” -Alan Watts
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Contam identification please. [Re: Amante]
#24578559 - 08/25/17 09:02 AM (6 years, 6 months ago) |
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If you make a LC you have to test it. We dont really bother to identify contaminants we toss them. Since it doesn't matter what it is.
Also if any one contamination occurs that means that any other contaminants could have made it in as well.
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Amante
The Chosen One



Registered: 07/09/17
Posts: 125
Loc: Somewhere over the rainbo...
Last seen: 3 years, 4 months
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Re: Contam identification please. [Re: bodhisatta]
#24578566 - 08/25/17 09:07 AM (6 years, 6 months ago) |
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My multiple jars are from different spore prints incase one did have contams. I understand most dont try to identify but my moto makes me curious. I beleive and intectual mind knows no bounds and fear is absent in understanding. We must perfect our knowledge of all that interest us, only then can we be happy. In that sense i like to know every detail of my hobbies. Thats why ive bene trying to figure out what the contam is, how it works, and what enviorments its predominant in. I will continue to research testing methods. Im very new to the hobby and these are my first lc's so sorry if i seem clueless.
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"Through our eyes, the universe perceiving itself. Through our ears, the universe is listening to its harmonies. We are the witnesses through which the universe becomes conscious of its glory, of its magnificence.” -Alan Watts
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Contam identification please. [Re: Amante]
#24578570 - 08/25/17 09:09 AM (6 years, 6 months ago) |
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Even if you buy a scope you'll get genus level identification if youre skilled, but probably not species level identification in most cases
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