Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineRushMore
persistant
Male User Gallery

Registered: 08/15/08
Posts: 16
Loc: Montana
Last seen: 5 years, 3 months
Opening LC Lab Help!
    #24570874 - 08/22/17 12:14 AM (6 years, 7 months ago)

So I have a request for technique's and or advice on working with my new LC Lab. First let me say that I have Bachelors Degree (heavy science background) a solid foot in Sterile Technique, and my last job was in an FDA Registered Lab at a pharmaceutical company. That said, I must also up-front confess that my experiences thus far have been largely in-line with the PF Tek from days of old and not anything heroic.
I purchased the Fungi Perfecti Master LC Lab with everything on it and had several issues upon delivery that were really unfortunate, but that's another subject entirely.
So I've never worked with an Eberbach or a LFH and I now have both. I had previously done my SAB work with very good results and could use some step by step pointers on working in front of the hood... I've done some video watching, but the thought of say pouring plates or knocking up dishes in a "Breeze" (sterile or not) is kinda freaking me out right now honestly.
And would the transfer from blender to glass be better performed in my SAB?
Seriously do not want to ruin many many jars with a bad LC...
Thanks in advance for any pointers you can send my way.


--------------------
-=When the Going Gets Wierd, the Wierd turn Pro=-

Edited by RushMore (08/22/17 12:33 AM)

Extras: Filter Print Post Top
Offlinedrake89
Mushroom Magnate
Male User Gallery


Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 5 years, 27 days
Trusted Cultivator
Re: Opening LC Lab Help! [Re: RushMore]
    #24571316 - 08/22/17 09:06 AM (6 years, 7 months ago)

So you bought a Ferrari without knowing how to drive a stick shift basically.


--------------------
Fiery Fungi (like us on faeboo)

Extras: Filter Print Post Top
Invisiblejkz
Padawan
I'm a teapot User Gallery

Registered: 06/16/13
Posts: 1,910
Loc: Coruscant
Re: Opening LC Lab Help! [Re: drake89]
    #24571542 - 08/22/17 11:24 AM (6 years, 7 months ago)

Quote:

drake89 said:
So you bought a Ferrari without knowing how to drive a stick shift basically.




Without knowing how to ride a bike is more like it.

No offence or anything Op, but why would you buy a LC lab if you've never made a LC before, or even done bulk?
This just sounds like a "more money then brains" type of scenario.

If you have a hood, it's better to use the hood then the sab I'd say.
LC is very particular, and you're probably going to ruin some stuff before you get it down. With that kinda lab or not. Failure is a part of learning.

Be prepared to lose grains dude. It's a part of it.
Start small, and do pint or half pint grain jars until you have your process down. And test every LC on agar before putting it to grain.


--------------------
TEKs I like
Trade list (inc)


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Opening LC Lab Help! [Re: jkz]
    #24573068 - 08/22/17 09:40 PM (6 years, 7 months ago)

My LC lab cost under 30 dollars.

Don't ruin a lot of jars. Test your LC and practice. Youll never get better if you dont fail a few times so get going

I have a BS too. A decade later and I suggest most people get a 4 year head start on life and avoid college.


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Offlinedrake89
Mushroom Magnate
Male User Gallery


Registered: 06/26/11
Posts: 4,168
Loc: TN
Last seen: 5 years, 27 days
Trusted Cultivator
Re: Opening LC Lab Help! [Re: bodhisatta]
    #24575903 - 08/24/17 07:43 AM (6 years, 7 months ago)

Quote:

bodhisatta said:


I have a BS too. A decade later and I suggest most people get a 4 year head start on life and avoid college.



If you want to do science why avoid college?  Where else are you going to have free access to millions of dollars in scientific equipment and people who are paid to teach you how to use it?


--------------------
Fiery Fungi (like us on faeboo)

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Opening LC Lab Help! [Re: drake89]
    #24576027 - 08/24/17 09:10 AM (6 years, 7 months ago)

Had i known better i could have got my foot in QA without a degree and could have got sent to school on someone else's dime for a BS in biology when I would have gave a shit.
I used way more cool equipment and learned way more about yeast and other microorganisms that contaminate  beverages in a real lab that had to keep a business in business.


--------------------
Welcome to the Advanced Mycology Forum

Bod's Library    Article behind a paywall? I can try to get it for you

Extras: Filter Print Post Top
Invisibleskuzzy1232
:))
Male User Gallery


Registered: 05/31/10
Posts: 1,460
Re: Opening LC Lab Help! [Re: bodhisatta]
    #24579755 - 08/25/17 08:04 PM (6 years, 7 months ago)



Run your hood for an hour before use  sterilize workspace with bleach . Etc


Basic sterile technique wipe everything down with alcohol before it goes in .

Operate close to the filter , never come between the filter air flow and what ever your working with.
Keep open air exposure to a minimum even in the "clean zone"

As far as pouring plates I just pressure cook the agar in a quart jar wrap a towel around it and transfer it to the flowhood to cool.  I line all the petri dishes up close to the filter. Then  alcohol swab down the jar . My jar has a simple hole on one side of the lid that was done with a nice pic and filled with poly fill before I pressure cook. It's hot, so remember to wrap a alcohol soaked towel or rag around it when you pour.  I just remove the poly fill with my left hand quickly remove the lid to the agar plate with my right hand poor and then put the lid back on with my left hand done .
Just remember to pour from the side and don't block the filter to the agar plate
.
Ps degrees or any schooling at all are not needed in this hobby. Neither is fancy equipment for that matter .


--------------------
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/8468463
Have any questions comments PM me ill try to help out the only stupid question is the one you dont ask .

Edited by skuzzy1232 (08/25/17 08:09 PM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   Myyco.com Isolated Cubensis Liquid Culture For Sale   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Cultivation Supplies


Similar ThreadsPosterViewsRepliesLast post
* PDYA LC question Lapius 1,849 5 07/06/05 11:23 AM
by Roadkill
* equipment questions/suggestions Anonymous 578 3 07/26/02 07:54 PM
by Anonymous
* Lc questions among other things xaxphaanes 2,526 16 11/10/05 02:16 AM
by jarroddupont
* Deleted Jared 1,683 19 06/16/01 02:30 AM
by Jared
* Constant-stir LC showing expotential growth in first 3.5 days. (pics)
( 1 2 3 4 5 all )
Blue Helix 27,902 96 07/31/05 09:07 AM
by scatmanrav
* Agar&LC
( 1 2 all )
Aiko Aiko 3,460 23 10/09/05 09:39 AM
by Blue Helix
* Open air grain to grain transfer idea..
( 1 2 all )
Sorted 7,368 20 06/06/03 07:52 PM
by FungusmaximusFM
* LC straight to bulk without grain
( 1 2 all )
StrophariaShaman 6,563 21 02/13/24 04:21 AM
by CapSlinger

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
800 topic views. 0 members, 1 guests and 6 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.006 seconds on 14 queries.