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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 478
Last seen: 3 days, 3 hours
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MEA vs PDA agar does it really need potato?
#24569857 - 08/21/17 04:29 PM (6 years, 6 months ago) |
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So I was looking g at the malt extract agar recipe vs the potato/dextrose agar recipe. Now malt is just a simple sugar just like honey so why does honey need potato and malt doesn't?
I'm thinking can't I just use honey and agar just like malt and agar?
Malt is hard to find around my area and when I make the potato/honey it makes the agar harder to see through makes it cloudy vs the malt/agar so if I just do honey/agar and leave the potato out then I'll have a nice clear agar dish to see through
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Shroomway
It's me!



Registered: 07/05/17
Posts: 438
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Re: MEA vs PDA agar does it really need potato? [Re: more4u2c]
#24570898 - 08/22/17 12:43 AM (6 years, 6 months ago) |
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PDA is lighter in color and thus even more see through then MEA. But it needs filtering probably to achieve that. I use cheese cloth (threefold). Works wonders. You get a very clear medium! You can't get dextrose? That is what keeps PDA nice and clear...
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foragedfungus



Registered: 09/30/13
Posts: 1,851
Loc: out there
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Re: MEA vs PDA agar does it really need potato? [Re: Shroomway]
#24571250 - 08/22/17 08:15 AM (6 years, 6 months ago) |
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Except malt is not "just a simple sugar".
" Due to the type of enzymes naturally present in malt, malt extracts have carbohydrate profiles very similar to a high-maltose syrup. Because they are made from a whole grain, they also contain about 6% protein (8% db), as well as an abundance of free amino acids, vitamins, and minerals." Quoted from- http://www.briess.com/food/Processes/nswhat.php
You can buy malt locally at beer brewing supply stores.
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more4u2c
Mad Hatter

Registered: 07/08/14
Posts: 478
Last seen: 3 days, 3 hours
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Re: MEA vs PDA agar does it really need potato? [Re: foragedfungus]
#24572119 - 08/22/17 03:20 PM (6 years, 6 months ago) |
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Yup found a nice big bag is light malt powder at the ballast point brewery. My agar is almost 100% clear this is going to be so much better than the potato/honey I'm beginng to think my PC was slightly Carmelizing the honey turning it darker.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: MEA vs PDA agar does it really need potato? [Re: more4u2c]
#24572379 - 08/22/17 05:09 PM (6 years, 6 months ago) |
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Quote:
more4u2c said: So I was looking g at the malt extract agar recipe vs the potato/dextrose agar recipe. Now malt is just a simple sugar just like honey so why does honey need potato and malt doesn't?
I'm thinking can't I just use honey and agar just like malt and agar?
Malt is hard to find around my area and when I make the potato/honey it makes the agar harder to see through makes it cloudy vs the malt/agar so if I just do honey/agar and leave the potato out then I'll have a nice clear agar dish to see through
Probably would help if you had your info right. Malt ain't simple. Its a complete media as is with all the necessary nutrients for fungi.
Pda needs potatos as just dextrose works poorly.
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: MEA vs PDA agar does it really need potato? [Re: bodhisatta]
#24572412 - 08/22/17 05:27 PM (6 years, 6 months ago) |
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Quote:
bodhisatta said:
Quote:
more4u2c said: So I was looking g at the malt extract agar recipe vs the potato/dextrose agar recipe. Now malt is just a simple sugar just like honey so why does honey need potato and malt doesn't?
I'm thinking can't I just use honey and agar just like malt and agar?
Malt is hard to find around my area and when I make the potato/honey it makes the agar harder to see through makes it cloudy vs the malt/agar so if I just do honey/agar and leave the potato out then I'll have a nice clear agar dish to see through
Probably would help if you had your info right. Malt ain't simple. Its a complete media as is with all the necessary nutrients for fungi.
Pda needs potatos as just dextrose works poorly.
How would just honey do as an agar recipe? I'm curious since when i make LC with 4% honey it colonizes a half full 1L bottle in 7 days completely thick.
Is there a big difference in how the mycelium digests in water compared to on petri dishes? Maybe I'm asking stupid questions in that case please forgive me!!
-------------------- Cakes inside Water Tub
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: MEA vs PDA agar does it really need potato? [Re: Mateja]
#24572417 - 08/22/17 05:29 PM (6 years, 6 months ago) |
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Honey LC works poorly compared to other media broth used as LC.
Its no surprise it colonized tho
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: MEA vs PDA agar does it really need potato? [Re: bodhisatta]
#24572437 - 08/22/17 05:39 PM (6 years, 6 months ago) |
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I usually go with your recipe 1.5% BRF Agar, I've germinated both cubes and Pan.Cyan on that recipe works great!
Now i want to try BRF LC, has anyone done it? What % should i try?
-------------------- Cakes inside Water Tub
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: MEA vs PDA agar does it really need potato? [Re: Mateja]
#24572439 - 08/22/17 05:41 PM (6 years, 6 months ago) |
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Brf LC seems like a bad idea. Imo LC is pretty useless for most cultivators when G2G is safer and faster. Unless youre trying to do a shitload of bags why bother with the extra step
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: MEA vs PDA agar does it really need potato? [Re: bodhisatta]
#24572450 - 08/22/17 05:48 PM (6 years, 6 months ago) |
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I am doing a shitload of BRF cakes
-------------------- Cakes inside Water Tub
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: MEA vs PDA agar does it really need potato? [Re: Mateja]
#24572465 - 08/22/17 05:54 PM (6 years, 6 months ago) |
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My unused Presto 23 PC does not work on my stove so until i solve that i have no use for the quart jars :/ I'm currently spawning BRF cakes to CVG with moderate success.
I'm dropping wedges into LC of course but still i know what you saying Bod!! I hope to do my first g2g really soon, even if i have to PC the quart jars two at the time with my IKEA PC.
-------------------- Cakes inside Water Tub
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mynakedrat
The phantom hourglass



Registered: 02/16/17
Posts: 4,629
Loc: Inner Astral levels
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Re: MEA vs PDA agar does it really need potato? [Re: Mateja]
#24572649 - 08/22/17 07:07 PM (6 years, 6 months ago) |
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How many is shit load?
More or Less than a fuckton?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: MEA vs PDA agar does it really need potato? [Re: mynakedrat]
#24573011 - 08/22/17 09:16 PM (6 years, 6 months ago) |
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Doing a shitload of cakes just make four spore syringes. That will easily do 50-80 cakes
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Mateja



Registered: 07/14/16
Posts: 7,948
Loc: Here
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Re: MEA vs PDA agar does it really need potato? [Re: bodhisatta]
#24573727 - 08/23/17 07:30 AM (6 years, 6 months ago) |
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I'm doing around 50 cakes but it's from a clone and not from spores. I actually don't have any spores left, maybe a couple of ml.
So should i instead make own spore syringes for BRF cakes instead of using LC?
If my sterile technique is equally good/bad when printing and making clean syringes as when making a clean LC, which of those are gonna be more reliable for BRF success? I don't mind a week extra colonization if the spores are gonna produce healthier cakes.
(I'm using BRF cakes as spawn)
-------------------- Cakes inside Water Tub
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foragedfungus



Registered: 09/30/13
Posts: 1,851
Loc: out there
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Re: MEA vs PDA agar does it really need potato? [Re: Mateja]
#24573764 - 08/23/17 07:58 AM (6 years, 6 months ago) |
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Quote:
Mateah said: If my sterile technique is equally good/bad when printing and making clean syringes as when making a clean LC, which of those are gonna be more reliable
If one cfu of mold or bacteria (colony forming unit- one spore, a single bacterium, a fragment of mold myc.) lands on your print, you might (or might not) suck it up into the syringe. If you use the syringes immediately that single c.f.u. will only be introduced to one jar.
If that same single c.f.u. gets in your LC it will reproduce in the nutrient broth. Creating whole populations, which can easily contaminate everything.
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