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 HerbBaker
Registered: 08/17/07
Posts: 2,506
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Vermiculite is not completely inert. Perlite however is.
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Some things can grow without the light.--RJD
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 PennyPincher
Nerd
Registered: 02/15/05
Posts: 28
Last seen: 12 years, 9 months
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 Re: clone from dried caps  [Re: HerbBaker]
#9553825 - 01/05/09 08:30 PM (15 years, 3 months ago) |
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I currently have 12-15 pieces of 2 year old dried caps and stems in PC'ed distilled water. The pieces were triple washed with PC'ed distilled water as per RR's "tek" found on several post. It has been 2 weeks now and the water has turned light brownish in color and filled with small floating blobs similar to past lc's that i've made. Several pieces have started to get a little fluffy, these will be transfered to agar. I'll try to get some pics. What do you all think the floaters in the water may be? Contaminants, mycelium from spores or the pieces?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Quote:
What do you all think the floaters in the water may be?
There's no way to tell.
Transfer the pieces with mycelium to agar, and be prepared to make a series of transfers to get live mycelium away from any contaminants. Since it's hard to tell contaminant fungi from mushroom mycelium, transfer each growth you see to new plates. That way, some will end up being contamination, but others will hopefully be mushroom mycelium.
RR
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semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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PennyPincher
Nerd
Registered: 02/15/05
Posts: 28
Last seen: 12 years, 9 months
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Re: clone from dried caps [Re: RogerRabbit]
#9558429 - 01/06/09 03:56 PM (15 years, 3 months ago) |
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RR, is this stuff floating in the water common when you use this method?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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I've seen mushroom tissue that floats and some that sinks. If that's not what it is, then I don't know.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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califarmer619
jardinero
Registered: 10/17/08
Posts: 255
Loc: your pussy
Last seen: 2 years, 11 months
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Re: clone from dried caps [Re: RogerRabbit]
#9594221 - 01/12/09 11:00 AM (15 years, 3 months ago) |
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hi all ive been reading this thread for some insight. I live in cali so I cant order any spores so I went ahead and bought some agar and petri dishes at the local teachers supply store. (ripoff) but anyways a buddy of mine gave me a dried mushroom of what looks like a pe strain. whell anyways I made up my petri dishes, and cut out some samples and but them on the dish. its been about 3 or 4 days and this is what I got. my question is "when do I cut out growth and transfer it onto another dish? hou long should I gro it out". thanks for your input
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Pinback
Stranger
Registered: 07/20/02
Posts: 836
Loc: Europe
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Sorry to say it, what you have there are bacteria and molds.
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,603
Loc: Oregon, USA
Last seen: 12 hours, 20 minutes
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It is a bit hard to tell from your photo, but the dark specks of mushroom tissue with just a bit of fuzz around them are likely the good stuff and look right considering the age. You should transfer away from the large slimy blobs (bacteria) immediately for best results. You may have to do this more than once before getting a clean culture. Good luck!
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lipa
Registered: 07/24/07
Posts: 2,684
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In case your confused with what Workman just told you. The two upper right pieces, big w/ small piece next to it. I would transfer the mycelium around the little piece. That would be a good start. After you transfer the mycelium place a sterile 3/4 inch by 3/4 inch piece of cardboard right next to the wedge and let the mycelium grow up above it then grab that mycelium with your loop.( this doesn't always work but most of the time it does) It will help separate it from bacteria if there is any present. I do this all the time with cultures that have bacteria in them and it works for me very well. Was there no gill tissue on the specimen you found? You can always just place a piece of gill tissue on the agar and try to get the spores on it to germinate.
Lipa
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califarmer619
jardinero
Registered: 10/17/08
Posts: 255
Loc: your pussy
Last seen: 2 years, 11 months
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Re: clone from dried caps [Re: lipa]
#9595545 - 01/12/09 03:20 PM (15 years, 3 months ago) |
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yea I had been gifted only one complete mushroom with the veil partially intact. does anyone know of a thread that has good picture of agar cultures or young ones for reference. thanks for your help
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califarmer619
jardinero
Registered: 10/17/08
Posts: 255
Loc: your pussy
Last seen: 2 years, 11 months
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heres a shot of amother dish
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Pinback
Stranger
Registered: 07/20/02
Posts: 836
Loc: Europe
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That looks excellent.
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Zelikoo
Stranger
Registered: 08/18/17
Posts: 14
Last seen: 2 years, 7 months
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 Re: clone from dried caps [Re: Pinback]
#24562733 - 08/18/17 12:36 PM (6 years, 7 months ago) |
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Hi!
Time to bump up this thread! I've just bought some dried mushrooms for the second time online and would just like someone to confirm with me if I can use any of these mushrooms/parts and just put on a petri dish with agar and with some luck it will start to produce mycelium which I then later can plant in a substrate?
[flash=,] [/flash]
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First Grow - 105g + 120g of Wet Shroomies!
     
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bodhisatta 
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: clone from dried caps [Re: Zelikoo]
#24563174 - 08/18/17 03:36 PM (6 years, 7 months ago) |
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You can make your own new thread too. But starting from dried is usually an uphill battle even for seasoned cultivators.
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vinsue
Grand Old Fart
Registered: 02/17/04
Posts: 17,953
Loc: The Garden State(NJ) 
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 Re: clone from dried caps [Re: bodhisatta]
#24567867 - 08/20/17 04:50 PM (6 years, 7 months ago) |
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13+ years ago. 
This was the first thread I ever made.
silly noob 
I never did clone those caps, just ate them and
went ahead and ordered some spore syringes.
  . . . 
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"All mushrooms are edible; but some only once." Croatian proverb. BTW ...
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