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Elegua
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Registered: 03/24/17
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Consistent problem with Pasty EzLC tek vs. Pastyplates
#24562501 - 08/18/17 11:01 AM (6 years, 5 months ago) |
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I've been working with agar in various improvised dishes -- Glad round Pastyplates, and small Ball Elite jars w/ LC lids as per the Pasty EzLC tek.
So far, the pastyplates have been quite efficient and effective for me, with no real major issues that couldn't be explained by various flaws in my technique as I go through the agar learning curve.
However, I keep getting the exact same problem whenever I use the glass jars for the agar-based EzLC: around the edge of the glass, I keep getting this odd appearance of roughness on the agar. Looking at the surface, it's like someone took rough sandpaper to a smooth surface, in a 1/4" ring right where the agar meets the side of the jar. It's colorless, and pretty much impossible to photograph in the jar (which is why I don't have any images), but I just keep getting the vibe that this is a bacterial infection.
Has anyone encountered this issue? Is there any chance it could be anything other than an infection (such as something about the glass altering the heating of the agar, leading to some kind of irregularity along the sides of the glass jar)?
And if you've encountered it, how did you address it in future attempts?
Thanks in advance...
Edited by Elegua (08/18/17 11:02 AM)
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JHOVA
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Elegua]
#24562506 - 08/18/17 11:02 AM (6 years, 5 months ago) |
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Pics
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Elegua
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Registered: 03/24/17
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: JHOVA]
#24562523 - 08/18/17 11:12 AM (6 years, 5 months ago) |
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OK I popped open one jar which has this particular development, as that was the only way to get any sort of photo -- it still doesn't really convey what I'm seeing very well...
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Elegua
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Registered: 03/24/17
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Elegua]
#24562524 - 08/18/17 11:14 AM (6 years, 5 months ago) |
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It looks like some kind of infection, I just can't figure why it's only happening with my glass jar plates w/ the LC lids. Bad lid? Bad seal?
Edited by Elegua (08/18/17 11:16 AM)
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Mirlin
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: JHOVA]
#24562527 - 08/18/17 11:14 AM (6 years, 5 months ago) |
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Could just be some mild clumping of sediments
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JHOVA
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Mirlin]
#24562564 - 08/18/17 11:29 AM (6 years, 5 months ago) |
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Can you post a perpendicular pic?
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BLINKfan420
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: JHOVA]
#24562583 - 08/18/17 11:36 AM (6 years, 5 months ago) |
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*EDITED*
It looks a little slimy to me which makes me think bacteria... But being that I'm not familiar with these teks AND it's A2A transfers I can't think of where the bacteria would come from.. especially if it's growing on the edges, away from the new agar wedges..
I'm guessing you followed the teks 100% and properly PC'ed the containers?
BLiNK
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  All my pictures are drawn from imagination. I don't even know what a mushroom is.
Edited by BLINKfan420 (08/18/17 11:42 AM)
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Elegua
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Registered: 03/24/17
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: BLINKfan420]
#24562592 - 08/18/17 11:39 AM (6 years, 5 months ago) |
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No, it's an agar transfer (off of Pastyplates). The only reason there's that extra fluff there on the side is because that particular jar had a fragment of the agar transfer break off just as I was transferring.
All my agar work on the PP5 rounds has been very consistent so far, it's just the glass doing this...thing.
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BLINKfan420
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Elegua]
#24562610 - 08/18/17 11:46 AM (6 years, 5 months ago) |
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Can you tell if it's growing/spreading? Also Its hard to tell from the pic, But is there any discoloration or is it always perfectly clear?
On the next batch maybe leave a jar or two as controls. If it doesn't pop up then you'll know it's your technique.
You're 100% sure the agar your transferred wasnt bacterial already? It can be hard to see sometimes.
BliNK
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WeavieWonder
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Mirlin]
#24562642 - 08/18/17 12:01 PM (6 years, 5 months ago) |
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Can't really say for sure whats going on in that jar.
You need a little more practice with agar work before progressing to LC. The agar shouldn't crumble apart like that. IMO, Pasty plates are great, but may not be the best way to start out. I say this because with regular disposable petris, they are a lot easier to visually inspect, and you don't have to open them to do so. Much better for pictures too should you need someone else to take a look at it.
Here is a pic of one of my recent petris. Infected with bacteria I believe. Slightly enhanced in photoshop.

Just something to think about. Good luck!
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Elegua
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Registered: 03/24/17
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: WeavieWonder]
#24562664 - 08/18/17 12:07 PM (6 years, 5 months ago) |
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Of course I can't guarantee that my other agar plates weren't infected before the transfer, but it just strikes me that this one particular problem only happens with the glass jars. I've seen infections on the PP5 rounds, and dealt with them individually, but it just never manifests in this particular fashion.
I will do what Blink suggests with setting aside an un-inoculated tester jar.
Oh, and so far it's always remained clear like this. Just those weird bumps.
Horizontal photo --

Edited by Elegua (08/18/17 12:13 PM)
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Elegua
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Registered: 03/24/17
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Last seen: 5 years, 3 months
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Elegua]
#24563109 - 08/18/17 03:13 PM (6 years, 5 months ago) |
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Oh, one thing I forgot -- I have taken samples from the same PP5 agar plate and transferred them to other pastyplates, and none of them have had the same particular issue of slick roughness right around the edges of the plate (or slick roughness in general...other contaminants, sure, but not that one). So far it's only the glass jars w/ LC lids that have given me this particular weirdness.
Edited by Elegua (08/18/17 03:32 PM)
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senseit
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Re: Consistent problem with Pasty EzLC tek vs. Pastyplates [Re: Elegua]
#24566378 - 08/19/17 11:22 PM (6 years, 5 months ago) |
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I can't see from the pictures too clearly.
Are your jars level when they are cooling? Try heating them up and letting them cool level. I have small glass jars that I use and if they are not level when they cool, the surface doesn't always turn out smooth. I think it might have to do with the curve of the bottom of the jar, but I'm not sure.
Edited by senseit (08/19/17 11:22 PM)
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