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InvisibleEdon
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24528397 - 08/03/17 08:19 PM (6 years, 6 months ago)

For killing bacteria in a culture, has anyone tried adding a small amount of copper?
That or pouring a very thin agar film over copper foil and trying to transfer mycelium to it.

Reason I bring it up is that hospitals are now starting to use copper more to replace stainless because the super bugs that are resistant to antibiotics die when exposed to copper.


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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: Edon]
    #24528444 - 08/03/17 08:39 PM (6 years, 6 months ago)

Never tried, but it's a very interesting idea to find out!

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OfflineQuadman
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24528780 - 08/03/17 11:04 PM (6 years, 6 months ago)

Copper breaks down proteins. Therefore it will kill bacteria , but also fungi and even plants. Copper sulfate is routinely used to control algae in ponds and lakes.


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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: Quadman]
    #24529313 - 08/04/17 08:10 AM (6 years, 6 months ago)

Ferather, I just realized that my "Reverse WL-tek" I proposed to you is actually pretty similar to your idea in utilizing paper pellets as vermiculite  :lol:
(except that if you use vermiculite you're adding soluble carbon into mineral based substrate while on paper pellets you're adding soluble carbon into carbon based substrate)

If you use cellulose as substitute for vermiculite and enriching it with soluble carbons and nutrients and utilizing it as substrate, should the condition kept sterile until fruiting?? Or the ph will do all the job for you??

Btw I think utilizing cellulose as spawn is a bad idea unless the condition during inoculation of spawn material kept sterile to keep contaminant spores getting in because they will activate when you add plant extract to enrich your spawn base. What do you think?

Edited by DailyShroomer (08/04/17 08:17 AM)

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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24529757 - 08/04/17 12:03 PM (6 years, 6 months ago)

"Grainless" Cellulose Based Spawn : Paper pellets enriched with plant extract, macro micro nutrients as needed, CaCO3. All sterile procedure, from inoculating into paper pellets to inoculating into substrate.

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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24529803 - 08/04/17 12:42 PM (6 years, 6 months ago)

Another idea would be to spawn your "cellulose cake" into lime treated straw.

Put some straw into plastic bags as base, then put mounds of your cellulose cake into the center and put more straw surrounding the cake. The end result will be similar to conventional straw logs.

Other substrates like cotton waste can be used as long as lime treated to adjust the ph.

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24529904 - 08/04/17 01:38 PM (6 years, 6 months ago)

I'm going to ignore some of the "over-excited" questions, mostly because you seem to understand it enough.

You can indeed use WL-Tek as spawn once it has fully colonized, or as final spawn expansion.
Forget the brown rot, white rot label, both types digest cellulose, doesn't apply.

Simply put, make grain spawn, spawn it to WL-Tek, expand or fruit it.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24529979 - 08/04/17 02:20 PM (6 years, 6 months ago)

Cellulose and starch are both composed of C6-H10-O5, in very different arrangements.
Here is some WL-Tek spawn to WL-Tek, using the cellulose agar alternative.

Pre-decay and active enzymes allow it to work like grain.

     

     


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24529998 - 08/04/17 02:29 PM (6 years, 6 months ago)

Here is some very "happy" mycelium, breaching the surface of WL-Tek.

   


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24530035 - 08/04/17 02:50 PM (6 years, 6 months ago)

Heavy Rain effect: hydration, waste removal and access to nutrients, also triggers pinning.

   

----

Here is some outdoor Golden oyster. Both are WL-Tek, cased in wood rich compost.
Even the last few flushes of a cake can be very beautiful, big and rich.

     

     

Ample O2 (fresh air), light and hydration.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24530104 - 08/04/17 03:14 PM (6 years, 6 months ago)

Additional notes:

Organic soluble nutrients and liquid nutrients will likely contain varied bacteria, sterilization is required.
Liquid seaweed products contain a few prolific cellulose digesting organisms, also sterilize.

I suggest dry nutrient additives, as they should already be aseptic (or enough).
Check the product label, add around 1-1.5g nitrogen per 100g paper.

Test small amounts of end media, such as 25g pellets.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24530258 - 08/04/17 04:02 PM (6 years, 6 months ago)

If you place a large enough tissue sample onto WL-Tek, it will grow out in about 4-5 days.
You can decide to take samples from it, by placing pegs or taking samples.

The tissue sample, if enough, will supply the starter units.



--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24530279 - 08/04/17 04:09 PM (6 years, 6 months ago)

Wild sample of Summer oyster on WL-Tek with added tea extracts.
I buried the tissue sample to lower the overall decay rate.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24530694 - 08/04/17 06:36 PM (6 years, 6 months ago)

Thank you, Ferather. Sorry if  a few, no.. I mean a lot of my questions are actually already answered from your experiments.  I tend to read and try to find answers for questions in mind but ignoring some useful facts, I'm a "scan and skim" type of reader. Thus, when I have few new questions popping I forgot that you actually have explained beforehand. I should have reread your teks and journal a bit more.

The "All Natural Fruiting Tek" holds all the answer for my recent question and I'm going to try it soon and I'll let you know. Thank you.

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24531856 - 08/05/17 08:33 AM (6 years, 6 months ago)

Absolutely no problem at all, good luck with your grows.

:thumbinator:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24534091 - 08/06/17 04:54 AM (6 years, 6 months ago)

I have another question, but this one is a new one. I might know the answer to the question already but because you're the one to come up with the tek so I might as well seek consultation about it.

So I just prepared WL-dishes yesterday and today I tried to clone from dried tissue. The tissue is ABM btw. The problem is dried tissues are notorious for holding large amounts of contaminant spores. I know, your carbon complex WL-substrate will handle those, mostly.

What I just realized is the contaminants might as well germinate in the dried tissues!

So, I thought maybe if I open the jars and incubate them outside in the shade with lots of FAE, it might deter growth of mold and other nasty stuff. And it also a quite fortunate coincidence since the species I'm working on really really REALLY loves FAE. The climate here also works well with this species. Since the WL-tek is specifically engineered to avoid spores germination, I think it would be a neat idea. What do you think??

Edited by DailyShroomer (08/06/17 04:57 AM)

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24534311 - 08/06/17 08:37 AM (6 years, 6 months ago)

If the mycelium you are handling can handle low temperature's you would be better off putting it into a fridge.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24534439 - 08/06/17 09:57 AM (6 years, 6 months ago)

Sadly no, fridge is out of the question. Any other suggestion?

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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: DailyShroomer]
    #24536554 - 08/07/17 09:34 AM (6 years, 6 months ago)

Hmm, I would get another container, the allows for full GE, and is filtered. You will still want GE and not FAE when colonizing.
Mycelium will respond to certain O2-CO2 levels as "grow out", e.g. fuzzy feet, underground or inside plant matter.

You can then put your sample into that container and put that outside, exposed might dry out too much.
There is also bugs and other possible issues (water logging) if it's fully exposed.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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OfflineDailyShroomer
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24559443 - 08/16/17 11:44 PM (6 years, 6 months ago)

I hereby declare that the WL-tek is not suited to clone dried mushroom tissue. But it was partly due to my greed that I used some of the outer part of the tissue, till I realized that nasties could germinate on the mushroom tissue itself. It really germinated lots of nasties and I dumped them away.

But, I really like your idea of adding CaCO3 to agar to prevent germination. So, I got a happening here :

I used "manure tea" as replacement for the regular tea because the species I'm working with is in the Agaricus genus, meaning they like to grow on decomposed substrates. Can you tell if the growth I just got is contaminant or mycelium?? Because it is circular and took a while to appear and also it's growing slowly. If it is indeed mycelium, I hope it is from spore germination, because the way I did this is by pouring hot agar over pieces of dried cap. And the reason I'm looking for spore germination is because I need birth of new organism instead of clone since store bought mushrooms are known to senesce and stuff. I also made a thread in case you haven't checked it out : https://www.shroomery.org/forums/showflat.php/Number/24557987

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