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OfflineThufir_Hawat
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24883296 - 01/01/18 03:04 PM (6 years, 2 months ago)

Quote:

Yesum said:
Quote:

Thufir_Hawat said:
Hope these cakes fruit, cause things are not looking good in the cat-knocked-over tub. The process unseated the substrate and left space that is now filled by slightly yellow water (metabolites from mycellium competing with bacteria I assume). Was hoping to see some invitro pins on the cakes but ended up birthing them the other day to roll and put in the sgfc. All from a completely untested culture so who knows if anything will appear. :undecided:




Hey man sorry about thinking you threw your cat off of the fridge. Lol. Sorry to hear about your tub as well.

My guess is you'll get something off your cakes. I don't think I ever not had a cake fruit. I don't think.



lol I love the image of me somehow standing on top of my fridge chucking a cat off while bent over in the 4 feet between fridgetop and ceiling.

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InvisibleYesum
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Re: The "Midnight Mycology" Thread. [Re: Thufir_Hawat]
    #24883475 - 01/01/18 04:50 PM (6 years, 2 months ago)

Quote:

Thufir_Hawat said:
Quote:

Yesum said:
Quote:

Thufir_Hawat said:
Hope these cakes fruit, cause things are not looking good in the cat-knocked-over tub. The process unseated the substrate and left space that is now filled by slightly yellow water (metabolites from mycellium competing with bacteria I assume). Was hoping to see some invitro pins on the cakes but ended up birthing them the other day to roll and put in the sgfc. All from a completely untested culture so who knows if anything will appear. :undecided:




Hey man sorry about thinking you threw your cat off of the fridge. Lol. Sorry to hear about your tub as well.

My guess is you'll get something off your cakes. I don't think I ever not had a cake fruit. I don't think.



lol I love the image of me somehow standing on top of my fridge chucking a cat off while bent over in the 4 feet between fridgetop and ceiling.




When u put it that way yeah the image of you bent over that way standing on your counter top probably. Lol


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OfflineFractal420
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24884506 - 01/02/18 07:02 AM (6 years, 2 months ago)

Personally i think MS > LC is a waste but agar to LC/LI is awesome. But knocking up MS grains and G2G, i feel theres a much better chance of some workable spawn than going the liquid route.

Lc is super fast when it works tho, so once you get some clean ones, its bomb. I just only got that far once and my LCs contammed a bunch of times from MS


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Grinning wide
And comforting me with it's three warm and wild eyes.

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InvisibleYesum
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Re: The "Midnight Mycology" Thread. [Re: Fractal420]
    #24884995 - 01/02/18 01:10 PM (6 years, 2 months ago)

I've yet to be successful at making a "clean" LC.
I have much more success just making LI


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InvisiblemushboyMDiscord
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24885037 - 01/02/18 01:26 PM (6 years, 2 months ago)

that sounds like your cultures are dirty. LI its just water. thats why LC is unforgiving.

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InvisibleYesum
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Re: The "Midnight Mycology" Thread. [Re: mushboy]
    #24885079 - 01/02/18 01:49 PM (6 years, 2 months ago)

Yes sir it does.

I even fucked up EZ LCs I mean come on.

Hey remember the clones I took that day. bro lost 2 plates to that yellow slime bacteria shit so I wasn't even trying to save those. But these. That's the tissue plates.
Making some transfers tonight.

Those were made.up on the 26th pretty sure


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InvisiblehamloafS
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24886250 - 01/02/18 10:32 PM (6 years, 2 months ago)

Inoculating LC with an agar culture that's been transferred at least three times from spores is a good route to take.


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InvisiblePterasteve
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Re: The "Midnight Mycology" Thread. [Re: hamloaf]
    #24886574 - 01/03/18 02:28 AM (6 years, 2 months ago)

Just spawned an assortment of tubs, trays, and shoeboxes. I'll post pictures in their respective threads after I get some sleep.

Tired...  :sosleepy:


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InvisibleYesum
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Re: The "Midnight Mycology" Thread. [Re: hamloaf]
    #24886656 - 01/03/18 03:59 AM (6 years, 2 months ago)

I haven't really tried making one since my agar works been better. I wanna get some whatmans before I try again.

When I finally do get around to making a LC. It's gonna be of cultures that's I wanna keep because there friggin potent, fast colonizing, and funky to grow.

Not because I'm trying to prove something to myself. Which at this point however. I probably am. Lol.


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InvisibleYesum
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24887933 - 01/03/18 05:26 PM (6 years, 2 months ago)

pulling a all nighter.
Changed it up a little with the wbs instead of oats.


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OfflineAcaterpillar
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24899829 - 01/09/18 01:55 AM (6 years, 2 months ago)

About to harvest my first proper flush from my tub, woooooh!!! :rockon:

Gonna try taking some clones and spore prints too.
Hope they come out clean :yesnod:


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OfflineFractal420
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Re: The "Midnight Mycology" Thread. [Re: Acaterpillar]
    #24899838 - 01/09/18 02:08 AM (6 years, 2 months ago)

Really i guess any MS culture is "dirty" more likely than not. Just a matter of how dirty. If clean= pure, 0-contam, transferred agar myc

I guess with grains, sometimes its not dirty enough to fuck up the project. Im sure theres always some bacteria. In LC made from Syringes as well


--------------------
Dreaming of That face again.
It's bright and blue and shimmering.
Grinning wide
And comforting me with it's three warm and wild eyes.

Prying open MY third eye


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Offline00Burnout
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Re: The "Midnight Mycology" Thread. [Re: Fractal420]
    #24899886 - 01/09/18 02:50 AM (6 years, 2 months ago)

Glad to see some life on this thread again. It's been a busy couple days for mycology work, I've pasteurized enough straw for 2 5gal buckets and spawned 3qt of blue oyster to each.

I still have to do 2 more buckets for my golden oysters. It's a major pain in the ass because my main burner went out, idk if I'll even be able to pc anything on my stove now. It's a wiring issue so it should be easy to fix if I can get to the short. If not it looks like I gotta get a propane burner and tank...yay spending more money...

I've got 9 jars of atl7 coming up on the 1 month mark. 2 jars are no good for stones, 1 contamed (mucor from what I can tell) and one hasn't colonized a small section of the bottom of the jar due to excess moisture (could be bacteria too). I had a really shitty sab sess and I'm glad I haven't lost more jars. I plan to case those 2 and fruit invitro in my gh.

As far as agar work, I have 4 golden oysters and 4 blue oysters on MEA, 1 atl7 in cold storage and 4 atl7 growing out on MEA as well.

I also have an atl7 lc I started a few days ago. I tried keeping the nutes low to increase clarity but I think I should've used at least a half gram more of malt extract. I might try grain water or brf lc next to see if I like it more.

**EDIT** I just "fixed" the stove, idk why I didn't think of this before. I just lifted the top and switched the front and back element connections and remarked which was which. Works better now than it did in the first place lol


--------------------
Peace, pot and microdot!:mushroom2:
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Edited by 00Burnout (01/09/18 03:10 AM)

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InvisibleYesum
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Re: The "Midnight Mycology" Thread. [Re: 00Burnout]
    #24902337 - 01/10/18 04:52 AM (6 years, 2 months ago)

I figured I'd share this. My first ever plates I poured the other day. I have a 1000ml. Media bottle. Made PDA and I managed to pour 39 plates. Lol. Not too sure if that's good or not.  Probably average. Not sure. Only had 4 plates with condensation. Couple plates had some air bubbles but not many. So all things considered. Plus being the first time I ever poured agar. I didn't do horrible I don't think.


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Offline00Burnout
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Re: The "Midnight Mycology" Thread. [Re: Yesum]
    #24902862 - 01/10/18 10:55 AM (6 years, 2 months ago)

39 plates is pretty good if you read bod's agar tek he pours 20 plates with a 500ml bottle. I rarely get 20 plates, but I like to pour a little thick cause sometimes I don't use all my prepared plates at once and some sit for a minute.


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Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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InvisibleAyePlus
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Re: The "Midnight Mycology" Thread. [Re: 00Burnout]
    #24902869 - 01/10/18 10:58 AM (6 years, 2 months ago)

I poured ~53 from 1L  last night, but usually about 50, I pour thin and carefully tilt the stack of dishes to spread the agar so they have nice even coverage


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InvisiblebodhisattaMDiscordReddit
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Re: The "Midnight Mycology" Thread. [Re: 00Burnout]
    #24902873 - 01/10/18 10:58 AM (6 years, 2 months ago)

I actually only put 400ml into the 500ml bottle.
20ml a plate give or take 400ml for 20

I used to pour some very expensive media. Cost per plate at over 3$

Wild yeast media. It inhibits saccharomyces cerevisiae growth but lets other saccharomyces species grow as well as a few other yeasts like candida species.
I would pour them super thin. Like get 40+ out of 400ml.

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Offline00Burnout
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Re: The "Midnight Mycology" Thread. [Re: bodhisatta]
    #24902892 - 01/10/18 11:08 AM (6 years, 2 months ago)

I use the same media bottle as you, so yeah 400-450ml is what I fill to. I'd love to see someone try to bring the full volume of the bottle to boil in a microwave...as long as it's not my microwave lol

I'd probably get more plates done if my house was even remotely level. If you sit in one of my kitchen chairs and lift your feet you will just keep spinning.

How's using a 1L in a sab? Seems like it would be a little harder to handle


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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InvisibleAyePlus
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Re: The "Midnight Mycology" Thread. [Re: 00Burnout]
    #24902953 - 01/10/18 11:40 AM (6 years, 2 months ago)

Quote:

00Burnout said:
I use the same media bottle as you, so yeah 400-450ml is what I fill to. I'd love to see someone try to bring the full volume of the bottle to boil in a microwave...as long as it's not my microwave lol

I'd probably get more plates done if my house was even remotely level. If you sit in one of my kitchen chairs and lift your feet you will just keep spinning.

How's using a 1L in a sab? Seems like it would be a little harder to handle




I use two 500mls, but I just upgraded to a FH soI recently  ordered a 1L off ebay, takes up less rack space is my thought.  I was pouring 50 in the SAB with only minor issues from the cramped workspace, failure rate of 1-3 everytime which I find acceptable as long as you make 2-3 transfers for every one you need..


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
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Offline00Burnout
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Re: The "Midnight Mycology" Thread. [Re: AyePlus]
    #24902973 - 01/10/18 11:54 AM (6 years, 2 months ago)

I normally only pour 10 plates at a time. Everything I have right now is clean and I'm not working on isolates so I don't have much need for agar work until my atl7 jars are done and my oyster buckets fruit.

After I get some good oyster cultures started from clones I'm ditching the ones I have, they're pretty weak.


--------------------
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No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
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