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Offlinetryptkaloids
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Registered: 02/08/15
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: natedawgnow]
    #24510588 - 07/26/17 09:42 PM (6 years, 7 months ago)

both of them or just the second one?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Invisiblenatedawgnow
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Registered: 02/09/15
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: tryptkaloids]
    #24510655 - 07/26/17 10:14 PM (6 years, 7 months ago)

It's probably not necessary at all, but I wouldn't put any in the first jar if you intend on actually soaking the
fruit for an extended period of time. You cut from the inside and conks are hard as wood so they're usually
pretty good to just cut straight from the center. I've done it a few times with no bacteria issues or other contams


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InvisibleLizardWizard
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: natedawgnow]
    #24511056 - 07/27/17 04:47 AM (6 years, 7 months ago)

Throw them in the freezer overnight, the tissue will probably
survive as they also survive in nature.



I did this with a maggot infested Hericium jar, worked perfectly.

You could also go back into the woods and do the toothpick method (stick it in at the edge, go back after a week or so, take it out, put in fresh ziploc baggie, put the colonized section to agar, clean up from there).


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The best things in life
can be smelled on one's fingers.

Edited by LizardWizard (07/27/17 04:48 AM)

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OfflineManifoldPrime
Per Ardua Ad Astra
I'm a teapot


Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 2 months
Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: LizardWizard]
    #24511080 - 07/27/17 05:20 AM (6 years, 7 months ago)

would that toothpick method work with storebought mushies? I was thinking of getting some shiitake cultures going since I'm bored but I'd also like to try this

wait I just realised that this wouldnt work :frown:

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InvisibleLizardWizard
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: ManifoldPrime]
    #24511103 - 07/27/17 05:49 AM (6 years, 7 months ago)

According to RR, storebought shiitake's never really work well because of senescence already being present or close.


--------------------
The best things in life
can be smelled on one's fingers.

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OfflineManifoldPrime
Per Ardua Ad Astra
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Registered: 03/16/17
Posts: 1,313
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: LizardWizard]
    #24511212 - 07/27/17 07:40 AM (6 years, 7 months ago)

What about doing a spore scrape on agar, would that reset the genetics?


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InvisibleLizardWizard
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: ManifoldPrime]
    #24511289 - 07/27/17 08:45 AM (6 years, 7 months ago)

Good fruiting cultures of Shiitake are very hard to find starting from spores, that's why everyone just takes the cultures from the suppliers. There are some cheaper suppliers out there, if you want I could link you one in PM?


--------------------
The best things in life
can be smelled on one's fingers.

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InvisibleNothingsChanged
Striving for Excellence
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Registered: 05/28/11
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: LizardWizard]
    #24512449 - 07/27/17 06:21 PM (6 years, 7 months ago)

Fucked off another culture(time constraints). Moveing backwards. Good thing it's for fun and set backs are part of the game.



The post about shabby or chic was excellent.


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: NothingsChanged]
    #24512460 - 07/27/17 06:25 PM (6 years, 7 months ago)

because it would have been soaking in its own filth all night :lol:

a common practice for cleaning tissue samples is to dunk them in sterile water. ive done as many as 3 baths before to clean up a wild reishi fruit

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Invisibleeatyualive
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: Munchauzen] * 1
    #24512628 - 07/27/17 07:26 PM (6 years, 7 months ago)

i confess, today was a good day. worked hard, played harder. busted out the old fender strat. played a little. been some time. the calluses aren't what they used to be.


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EAT GETS SHIT DONE


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InvisibleLizardWizard
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: Munchauzen]
    #24512634 - 07/27/17 07:28 PM (6 years, 7 months ago)

Indeed, you basically do the same as with a spore print to spore syringe, you dilute the contaminants, only with spores, you can't just throw away the water so you add more.


--------------------
The best things in life
can be smelled on one's fingers.

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Offlinemushroom_therapy
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Registered: 03/23/17
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: LizardWizard]
    #24512714 - 07/27/17 08:06 PM (6 years, 7 months ago)

i confess ive been so busy with my new job, school, and helping my girl move, i havent had time to deep clean my place yet...i think im gonna buy a second larger hepa filter and have that run 24/7 as well though. Ive got 5 healthy looking plates in my fridge i wanna transfer


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Speak to me in energy, that way I can understand you better.


ALSO...check out this fellow shroomites dope tunes...
LOBIS' soundcloud playlist

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InvisibleBoogieman47
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Registered: 03/05/16
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: mushroom_therapy]
    #24512783 - 07/27/17 08:40 PM (6 years, 7 months ago)



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OfflinePsiloPsychIn
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Registered: 06/17/14
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: Boogieman47]
    #24515552 - 07/29/17 07:14 AM (6 years, 7 months ago)

True Confession:

I'm going on vacation to Hawaii with my wife...
I'm really excited to hang with her there, but I'm also excited by the possibility I may find some pan cyans to print!

:awedance:


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What are they saying? Listen carefully, it might be something you need to hear...




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InvisibleLizardWizard
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: PsiloPsychIn]
    #24515612 - 07/29/17 07:55 AM (6 years, 7 months ago)

If you get the chance to print a bunch, I'd love one :yesnod: :begger:


--------------------
The best things in life
can be smelled on one's fingers.

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Offlinemushroom_therapy
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Registered: 03/23/17
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: PsiloPsychIn]
    #24516031 - 07/29/17 11:42 AM (6 years, 6 months ago)

Ay badass!!! What island? I go to Kauai every year!


--------------------
Speak to me in energy, that way I can understand you better.


ALSO...check out this fellow shroomites dope tunes...
LOBIS' soundcloud playlist

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InvisibleNothingsChanged
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: PsiloPsychIn]
    #24516193 - 07/29/17 01:17 PM (6 years, 6 months ago)

Quote:

PsiloPsychIn said:
True Confession:

I'm going on vacation to Hawaii with my wife...
I'm really excited to hang with her there, but I'm also excited by the possibility I may find some pan cyans to print!

:awedance:





https://www.shroomery.org/forums/showflat.php/Number/17854241#17854241


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OfflineDoc9151M
Mycologist
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Registered: 02/23/17
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: mushroom_therapy] * 1
    #24516203 - 07/29/17 01:22 PM (6 years, 6 months ago)

Can u hide me in your luggage? I can fend for myself once we get there.


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Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593

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Invisibleeatyualive
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: Doc9151] * 1
    #24516345 - 07/29/17 02:41 PM (6 years, 6 months ago)

i confess, i cranked out the myco work last night so i could enjoy the weekend!


--------------------
EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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InvisibleBoogieman47
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Registered: 03/05/16
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Re: TRUE CONFESSIONS - of an amateur mycologist (what was deleted?) [Re: eatyualive]
    #24516363 - 07/29/17 02:47 PM (6 years, 6 months ago)

I confess im fucking pissed about some of my plates some are lookin shitty .. i think one issue may be the pasty lids are a little loose and with the fan bacteria is getting up and under


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