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OfflineDrMushroom
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Is just diammonium phosphate good enough to replace yeast nutrient in PDYA
    #24510647 - 07/27/17 12:11 AM (4 years, 3 months ago)

the main active compound in the various nutrients yeast powder contains which is utilized by our mushroom mycelium in PDYA agar cultures, is diammonium phosphate (DAP).
There is other compounds too, miscelaneous vitamins and minerals like zinc.

Is it possible to substitute yeast powder (the nutrient enhanced kind we use), with just its equivalent DAP content? or will excluding those other compounds have a significant impact on the speed and strength of the culture?


And also, while im asking about PDYA, what would happen if you added DAP to MEA agar? would it have a substantial boost or does the malt address the nitrogen and phosphorus needs of the culture on its own?


I ask this because i have a 25kg drum of DAP and recently remembered that DAP is the main ingredient in yeast nutrient, and i hate filtering yeast thickened water to get nice clear agar plates when doing PDYA.


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OfflineWiiiiilson
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Registered: 06/07/17
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Re: Is just diammonium phosphate good enough to replace yeast nutrient in PDYA [Re: DrMushroom]
    #24511193 - 07/27/17 09:21 AM (4 years, 3 months ago)

I didnt think most fungi could (agaricales) metabolise inorganic phosphates? MEA is pretty much a complete nutrient source, as far as I know and is probably a better option than adding DAP to PDY agar.
DAP is better for plants. I think adding a little malt extract to a potato dextrose solution would boost significantly boost the vitamin and mineral content. However, for agar, just potato dextrose alone should be enough.

If the purpose of the agar is to isolate away from bacteria and other fungi, I think a simple PDA is best - adding a nitrogen source to the mix is really going to give any bacteria a significant advantage.


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