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OfflineThenewbie321
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Registered: 01/08/17
Posts: 12
Last seen: 4 years, 11 months
Re: Cultivation General Discussion [Re: hamloaf]
    #24505250 - 07/24/17 03:45 PM (6 years, 7 months ago)

Hey yall i need some help with my grow. I had success, and had 6 strains, then tried to copy them to agar, to which i failed. I stopped using rye grain and started using wbs but im still not haing success. I think its the lids on my jars because i am following the grain tek to a t and sterilized my syringe following another tek. My syringe is still good and so is the lc that i pulled from so i am certain my box is working fine, and that it must be my lids or my grains. Im using a self healing innoc port with polyfil in the other hole. Any advice guys? starting to lose hope here.

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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Etnospace] * 1
    #24505259 - 07/24/17 03:50 PM (6 years, 7 months ago)

Quote:

Etnospace said:
Quote:

bodhisatta said:
Directly inoculating manure won't work



can you explain why its not gonna work ,please? just interesting



No,
People tried it and still try it all the time. Doesn't work. that's why we know it doesn't work.
No one has a lab or the desire to find out why

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OfflineDaken
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Registered: 07/24/17
Posts: 6
Last seen: 6 years, 6 months
Re: Cultivation General Discussion [Re: bodhisatta]
    #24505269 - 07/24/17 03:54 PM (6 years, 7 months ago)

Can anyone  help me with a question about using rye grain

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OfflineDaken
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Registered: 07/24/17
Posts: 6
Last seen: 6 years, 6 months
Re: Cultivation General Discussion [Re: Daken]
    #24505292 - 07/24/17 04:07 PM (6 years, 7 months ago)

I inoculated my rye grain in my all in one grow bag and it's is turning a whiteish color but seems rather soggy instead of wispy . I thought  maybe it was from introducing  heat raising the humidity and the water settling towards the bottom (the old mycopiss) because there is no smell  but am unsure is anyone familiar with this and does it sound like a problem.

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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: Daken]
    #24505324 - 07/24/17 04:24 PM (6 years, 7 months ago)

pics are awful but looks milky like bacteria and other nasties:uhoh:

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OfflineDaken
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Registered: 07/24/17
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Re: Cultivation General Discussion [Re: mushboy]
    #24505390 - 07/24/17 04:52 PM (6 years, 7 months ago)

It is rather milky and has been almost 2 weeks like I said no bad smells spore syringe looks rather light on spores could perhaps no spores taken root and solution cased this? Will try and get better pics

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OfflineDaken
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Registered: 07/24/17
Posts: 6
Last seen: 6 years, 6 months
Re: Cultivation General Discussion [Re: Daken]
    #24505410 - 07/24/17 04:59 PM (6 years, 7 months ago)

Hopefully better pics
[=https://files.shroomery.org/files/17-30/093707782-IMG_20170724_185450.jpg][/url]

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InvisibleJosex
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Re: Cultivation General Discussion [Re: Daken]
    #24505415 - 07/24/17 05:02 PM (6 years, 7 months ago)

That looks toast.

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OfflineDaken
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Registered: 07/24/17
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Last seen: 6 years, 6 months
Re: Cultivation General Discussion [Re: Josex]
    #24505427 - 07/24/17 05:08 PM (6 years, 7 months ago)

I was thinking same thing any idea as to what may have caused it ordered spores and bag from reputable sources followed all procedures, bad inoculation, bad spores they seemed very light too much heat. Just so I can adjust my procedure and avoid wasting money more failed materials.

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OfflineDaken
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Registered: 07/24/17
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Last seen: 6 years, 6 months
Re: Cultivation General Discussion [Re: Daken]
    #24505437 - 07/24/17 05:14 PM (6 years, 7 months ago)

Also could the bag be restralized and inoculated again.

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OfflineDA Double G
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Registered: 06/16/17
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Re: Cultivation General Discussion [Re: Daken]
    #24506085 - 07/24/17 10:23 PM (6 years, 7 months ago)

I think if you try that, it will throw off the moisture content. I know you don't want to lose what you spent, but I never had much luck with any pre-mixed substrates. Might be best to take a loss on this one.
If you have some spores left, might give this a try https://www.shroomery.org/forums/showflat.php/Number/19208976
Its helped me alot,
Then when you get some good cultures, put them to grain jars.
Multi-spore inoculation can be a gamble, but using agar has really helped me obtain clean mycelium, so I know what I'm putting in my jars rather than hoping what's in the syringe is good


--------------------
Now they've found me, at last they've found me, it's hard to run from a starving family. Now they've found me, I won't run. I'm tired of hearing, there goes a well known gun.

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OfflineKenetic
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Re: Cultivation General Discussion [Re: DA Double G]
    #24506415 - 07/25/17 02:26 AM (6 years, 7 months ago)

Gotta diy man.  I'd start with jars, not presterilized bags.  I actually started the same way you did and it never worked for me (plus I got kicked out of the house when they found out, stupid teen lol). 

pf tek or wait until you move out of your parent's house lol

rye sux also imo (used it exclusively for years).  It's dirty as fuck and easily contams.....


--------------------
Todo Cambia
   

               
                                                :cookiemonster::elmo:



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Offlinedylatov.pass
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Registered: 07/22/17
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Re: Cultivation General Discussion [Re: Inocuole]
    #24506771 - 07/25/17 09:15 AM (6 years, 7 months ago)

can i make an inoculation syringe from an old brf cake?

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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: dylatov.pass] * 1
    #24506799 - 07/25/17 09:35 AM (6 years, 7 months ago)

No, you can make one from spores from a mushroom though

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OfflineKyshroomer19xx
Est 19xx soldier
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Re: Cultivation General Discussion [Re: bodhisatta]
    #24506897 - 07/25/17 10:18 AM (6 years, 7 months ago)

I need some info on making a sterile spore syringe. Can I just scrap the spores in a shot glass and use distilled water squirt it in the shot glass an suck it up in the syringe? I don't have a PC can I just boil it in water or steam sterilize it?


--------------------
RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore

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InvisibleBoogieman47
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Re: Cultivation General Discussion [Re: Kyshroomer19xx] * 1
    #24506959 - 07/25/17 10:38 AM (6 years, 7 months ago)

Spore syringes are never sterile you can aspirate the syringe with boilimg water a few times to kill off any bacteria inside of it and just do the shot glass but you only need to use like a tiny corner of the spores like the size of a pea


--------------------

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InvisibleGerms
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Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #24506961 - 07/25/17 10:39 AM (6 years, 7 months ago)

Quote:

Kyshroomer19xx said:
I need some info on making a sterile spore syringe. Can I just scrap the spores in a shot glass and use distilled water squirt it in the shot glass an suck it up in the syringe? I don't have a PC can I just boil it in water or steam sterilize it?



No such thing as a sterile spore syringe. Spores are inherently not sterile. Boil the water, it's what I do


--------------------

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InvisiblebodhisattaMDiscordReddit
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Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #24506963 - 07/25/17 10:40 AM (6 years, 7 months ago)

Quote:

Kyshroomer19xx said:
I need some info on making a sterile spore syringe. Can I just scrap the spores in a shot glass and use distilled water squirt it in the shot glass an suck it up in the syringe? I don't have a PC can I just boil it in water or steam sterilize it?



Watch the lets grow mushrooms spore syringe TEK

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OfflineKyshroomer19xx
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Re: Cultivation General Discussion [Re: Germs]
    #24506978 - 07/25/17 10:44 AM (6 years, 7 months ago)

Ok cool do I really need to put lube in the water I plan to use it a day or 2 after I make it?  So I just mix the spores in distiller water suck it in the syringe and boil it or do I boil it before hand so i don't cook the spores? I just watched that video after I posted  that but I am still a little unsure since I have no PC


--------------------
RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore

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OfflineManifoldPrime
Per Ardua Ad Astra
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Registered: 03/16/17
Posts: 1,313
Loc: South Africa Flag
Last seen: 1 year, 2 months
Re: Cultivation General Discussion [Re: Kyshroomer19xx]
    #24507020 - 07/25/17 11:02 AM (6 years, 7 months ago)

The lube isnt necessary just helpful. Mine are done sans lube and I have good success. I tend to make 6-8 syringes per big print.


.
Boiling store bought Distilled water should get it sterile *enough*. Willy Myco has a method of placing a bowl/jar in a saucepan with water in it, and letting the steam collect in the bowl. I wouldnt do this considering the bowl would have to be pristine. My personal method to get water is to just pressure cook glass jars with silicone ported lids.

If you wanna get into mycology seriously , get your mitts on a pressure cooker. Ask your family if they have one, say you wanna make a hearty stew:hehehe:

If you can, get into agar, so you can directly apply spores with an inoculation loop to the agar(I make ghetto ones with paperclips that get flame sterilised), this is much more efficient in terms of germination.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

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Shop: Left Coast Kratom Buy Kratom Extract   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Grow Bags   North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale


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