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Offlinejimmyjimjames
blocking the sun

Registered: 06/23/17
Posts: 20
Last seen: 6 years, 6 months
cloning / refining strategy question
    #24504010 - 07/23/17 10:38 PM (6 years, 8 months ago)

My plan is to grow out 4 MS monotubs, then do some refining via cloning. What makes intuitive sense is to only take samples from the prettiest fruits of the most successful fruiting tub, and then narrow down on potency with subsequent grows. Is this a good plan, or are there better selection methods? When does it make sense to begin using slants for storage and reference? Thanks.

Also, is there much benefit from pursuing an isolate over a successful multi-strain clone lineage?

Edited by jimmyjimjames (07/23/17 10:50 PM)

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OfflineB-nizzle
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Registered: 07/23/17
Posts: 2
Last seen: 6 years, 5 months
Re: cloning / refining strategy question [Re: jimmyjimjames]
    #24504049 - 07/23/17 11:09 PM (6 years, 8 months ago)

That's basically how you create a master culture. Yes, do it. Every section of Rhizomorphic mycelium will carry specific genes that can be isolated. When you fully cultivate the master culture let me know. Extremely excited to see how you do.

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Offlinejimmyjimjames
blocking the sun

Registered: 06/23/17
Posts: 20
Last seen: 6 years, 6 months
Re: cloning / refining strategy question [Re: B-nizzle]
    #24504102 - 07/23/17 11:45 PM (6 years, 8 months ago)

Any suggestion on how many samples to take from a tub?

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InvisibleBoogieman47
Let's boogie
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Registered: 03/05/16
Posts: 9,712
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Re: cloning / refining strategy question [Re: jimmyjimjames]
    #24504163 - 07/24/17 12:27 AM (6 years, 8 months ago)

You take however many clones you like you test potency after the sample has been moved to agar ... once you have that culture cleaned you grow it out if its shit toss it cloning a clone is a waste of time because you already have the genetics ...


I will clone a clone if my cultures get fucked up somehow but from the time you clone it till the time you stop using it it will pretty much be the same


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