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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24471828 - 07/10/17 06:13 AM (6 years, 7 months ago)

Yes, any live sample, eventually the tissue will decay, but you should get transferable growth.


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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24471838 - 07/10/17 06:26 AM (6 years, 7 months ago)

Can you use substitute for CaCO3 like with powdered eggshell for example?? I totally want to try this out.

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24471860 - 07/10/17 06:43 AM (6 years, 7 months ago)

Yes, egg shell is 95-97% CaCO3, other names: Chalk, Limestone, Oyster shell, Egg shell, Agricultural lime.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24471981 - 07/10/17 07:43 AM (6 years, 7 months ago)

Sweet!!

By the way, have you seen this? https://www.shroomery.org/forums/showflat.php/Number/17356799

Is the T-agar essentially the same as the link I'm referencing or is that just some sort of upgraded PDA?? It will be more efficient to use pour agar tek I think if you have smaller PC I think.

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24471995 - 07/10/17 07:51 AM (6 years, 7 months ago)

Thanks for the link, no the T-Gel recipe is not based of that. The recipe is based on carbon content and enzymes.
However, I admit both the tea extract and CaCO3 are considered antibacterial, so yes there's a bonus.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24472044 - 07/10/17 08:22 AM (6 years, 7 months ago)

Can you use pour instead of no pour for T-gel like from my link?? I have a small electric PC, if can I'll buy loads of 45ml glass jars and start with them since it would be uber efficient to not PC the jars and just let the tea, CaCO3 and heat to do the work.

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24472060 - 07/10/17 08:32 AM (6 years, 7 months ago)

Yes, pour or no pour, entirely up to you. Handle as you would with any other agar.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24472083 - 07/10/17 08:50 AM (6 years, 7 months ago)

Sweet! :wink:

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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24472085 - 07/10/17 08:52 AM (6 years, 7 months ago)

Oh, one more thing! What's the consensus on green tea?? Is it workable with the beef gelatin and the CaCO3??

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24472089 - 07/10/17 08:56 AM (6 years, 7 months ago)

Yes, a few other community members have tried green tea, and got positive results.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24480435 - 07/13/17 02:34 PM (6 years, 7 months ago)

I added my transfer pegs yesterday, it has already begun transferring.

       


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24486654 - 07/16/17 05:56 AM (6 years, 7 months ago)

The medium is more populated than I expected, as in there is not many gaps between the growth, a wide spread.
There is also signs of genetic differences, several areas are much faster and denser than others.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24486657 - 07/16/17 05:56 AM (6 years, 7 months ago)

In conclusion, my T-Gel agar recipe works as intended, without sugar, starch and cellulose contamination dropped to none.
I suggest assembling and working with it in a SAB, but open air is more than doable without a SAB-other.

Only organisms capable of re-assembling materials into essential sugars will grow.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24488421 - 07/16/17 09:33 PM (6 years, 7 months ago)

This T-Gel works best for dirty wild samples, right? Oh, btw about green tea. How much green tea powder for 125 gr of CaCO3 water?? Green tea usually comes in dissolvable powder and I don't think 12 gr per 125 gr water is a realistic ratio, that'll be too much tea.

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24489133 - 07/17/17 09:32 AM (6 years, 7 months ago)

Any dirty sample, wild or not, the recipe I am using uses 1 tea bag, which is 3.25g.
Given only the extract is used, I'd say that is up to 1g of extract, roughly.


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Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineDailyShroomer
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24495218 - 07/19/17 10:34 PM (6 years, 7 months ago)

*noted

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: DailyShroomer]
    #24495723 - 07/20/17 05:53 AM (6 years, 7 months ago)

No problem, the recipe is cheap and easy to make, and works like other antibiotic agar's.
I suggest pH strips, you can add lime to the tea extract to pH 7-8 (oysters).

Then add the mixture to the dry agar and 240B gelatin.

:thumbinator:


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24496149 - 07/20/17 11:28 AM (6 years, 7 months ago)

I'm trying another recipe, using just CaCO3 as the primary carbon source.

125g > Boiling hot water + 10g CaCO3.

0.1g > MG soluble nutrients.
0.2g > 240B gelatin.
2.5g > Agar.


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather]
    #24496152 - 07/20/17 11:29 AM (6 years, 7 months ago)

I am transferring one of the pegs I produced using the T-Gel recipe.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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OfflineFerather
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Re: Antibacterial Tea Agar [Re: Ferather] * 1
    #24500279 - 07/22/17 06:27 AM (6 years, 7 months ago)

The C-Gel agar is working, faster than T-Gel agar, and about the same speed as malt extract agar.
Both the MG solubles as nutrients and CaCO3 as carbon, are working as I intended.

Growth is slightly thicker than T-Gel, however still radial as normal.

   


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

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