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InvisiblebodhisattaMDiscordReddit
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Bod's proper spore printing TEK * 59
    #24461957 - 07/06/17 12:52 PM (6 years, 8 months ago)

This tek is part of bod's cultivation is easy as fuck link list


Bod's Proper Spore Printing TEK


For making high quality dark spore prints for personal use and trade. A double printing technique is employed for spore prints for trade and prints that will be made into syringes. Even without this extra precaution you'll find the first of the two printings is suitable for agar use with a very low contamination rate when plated on agar.





You will need


A SAB(still air box) or LFH(laminar flow hood)
A roll of heavy duty foil
A spray bottle with sanitizer(70% iso)
A scalpel with a fresh sharp blade
Disposable powder free gloves
Sporulating mushrooms, use second flush or third flush ones for larger prints.


Even though it only takes two spores and a small light print will do, the bigger and darker the spore print the more spores there are. This out-competes any extraneous contaminant spores that may be present. If there are 10 rogue spores on your print. Would you rather have 1,000,000 cube spores or 100 million? Each time you swab that print you'd have a 10 to 1 million ratio or a 10 to 100 million ratio of spores to contaminants.

To make spore prints for trades or to people who may not have access to agar or haven't gotten there yet in proficiency. You will make two prints from one cap, and use the second one for those circumstances. The logic behind this is that the caps of the mushrooms grown and transported through open air have accumulated some rogue mold spores. The first print is taken for 4-6 hours and any rogue contaminant spores should be dislodged on to the first print. This first print should contain more of the rogue contamination than the second print will. In practice this first print still works very well. Save these first prints for yourself and agar work. The second spore print will take 12-24 hours to develop a nice dark print. This second spore print can be assumed to be even more contaminant free and is good choice for riskier methods like making spore syringes in lieu of agar work.

How to do it


This TEK's examples were made in a SAB

First you'll want to get some sporulating mushrooms


Set up your SAB as you normally would.
I wipe the table down with a clorox or alcohol wipe and the inside surfaces of the SAB then put something heavy on the top so it cannot slide around.

Since I'm not using a torch I lightly spray the inside of the SAB with the 70% iso this helps the foil stick to the floor better and has nothing to do with making the SAB environment more clean. The SAB works by virtue of it's design.

Put some gloves on and spray your hands down with your 70% iso alcohol.

inside the SAB lay out a piece of foil this will be the new floor.

then lay out a piece of foil on top of that. Foil comes sterile off the roll. the floor piece was removing the first roll of foil off the roll, the second sheet is our printing surface which has been protected from contamination by the outer roll of foil we used as the floor.

Cut the caps off your mushrooms and peel off any veil remnants that are in the way of the gills. I spray the scalpel with iso, I see no point in flaming the scalpel to cut the caps off mushrooms grown in open air. The second printing technique takes care of any worrying contaminants.

Then place your caps onto the printing foil


Lay the caps only on the bottom half of the foil. You'll be folding the foil in half to cover the print later.

I can comfortably fit about 9-12 prints of large 3-5 inch caps in my 110q SAB at a time with room to eventually transfer them to new foils and let the first ones dry.

Let these first ones print for 4-6 hours. loosely tent the prints with another piece of foil.

while you're printing cover the holes of your SAB

push this to the back of your sab. After 4-6 hours put the tent aside and remove the caps to a newly prepared printing foil in the front of the SAB.


once you remove the caps and put them on to new foil for the 2nd printing round you'll notice your prints have some moisture on them. They will need to dry before you fold them up. I put the prints in the back of the SAB to dry, loosely tented with the same tent you used when they were printing


The second round of printing will take 12-24 hours to develop a dark print. You can take the dry first prints out and fold them up or make a new tent for the second round prints and then fold them all at once. The spent mushroom caps can be then eaten right away or used to make tea.


your prints should look like this once dry

Your printing foils will resemble this diagram


first you'll fold your foil in half to cover the spore prints. Then fold in between each print and you'll be able to tear the individual prints away. I would avoid using scissors with anything having to do with print making. Why cut through something with a sanitized interments when you can chose more sterile steps to take.

Once the foils are all cut out you'll have squares with the print on the inner surface protected by the sterile foil

Fold them up like this



The sides first then the top over



Flip it over and label it immediately to avoid confusion later on


While this method may not be the most time and space efficient for spore prints. You will obtain a superior product make with care and mindfulness. You will avoid sanitation when possible in favor of taking more sterile steps. This more exacting and careful aseptic technique will be appreciated by all who receive your prints. 

Things to be aware of to make a proper print

Foil comes sterile off the roll.
You can sterilize foil in the oven if you compromise them or want to cut foil to size first.

Chemicals sanitize. Do not wipe foil with alcohol or bleach to try to sterilize it. It won't be sterilized. It will only be sanitized, we want sterile foil to print on.

Don't print more than one row of caps at a time. To do multiple rows you'll want to tent one row as you go. Don't put your non sterile hands over anything you want to keep aseptic.
Even with a flow hood you don't want to hover your hands over the tops of other prints you have taken.

if you print multiple rows like that you not only have to move your hands over areas you shouldn't.
Even with a flow hood caps closest to the flow will blow on the caps further away as well.
Also, while cutting out multiple prints on foil it will be hard to do so aseptic without introducing contaminants even if utilizing a hood.
Printing a single row on foil allows you do dry your prints then fold them over to keep them protected. Once they're protected by folding over you can aseptically cut or crease and tare apart individual prints.

If you're printing with a flow hood don't put sanitized instruments or surfaces in between the flow and your prints. You'll want your prints to be closest to the flow so that nothing can blow onto them from surface that has only been sanitized.


Prints should be folded over so that the print itself is not exposed.

Exposed prints are a hassle for people down the line and to remove them from a baggie can wipe off spores. They're harder to keep contamination free for multiple uses when done like that.



More information about mushroom spores

Spore discharge and dispersal in mushrooms

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OfflineCarmodycaramel
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Re: Bod's proper spore printing TEK [Re: bodhisatta] * 4
    #24461980 - 07/06/17 12:59 PM (6 years, 8 months ago)

You are smashing with all of these teks man, loving it!


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Invisiblejkz
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Re: Bod's proper spore printing TEK [Re: Carmodycaramel] * 2
    #24462400 - 07/06/17 03:30 PM (6 years, 8 months ago)

Good shit.
Can't go wrong with a step by step guide.
I've gotten some prints done this way, they're beauty.


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Offlinecatnip40
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Re: Bod's proper spore printing TEK [Re: jkz] * 2
    #24465536 - 07/07/17 04:01 PM (6 years, 8 months ago)

:takingnotes:

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InvisiblemushboyMDiscord
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Re: Bod's proper spore printing TEK [Re: catnip40] * 4
    #24465625 - 07/07/17 04:35 PM (6 years, 8 months ago)

nice.

that packaging is amazing. can you make a video of how you do that for us morons? :shrug:

i love dark prints.

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InvisibleZiran
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Re: Bod's proper spore printing TEK [Re: bodhisatta] * 5
    #24465731 - 07/07/17 05:10 PM (6 years, 8 months ago)

:thumbup: i knew you'd make one if I did too.


--------------------
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Ziran's Teks
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Offlinefatboyjeff
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Re: Bod's proper spore printing TEK [Re: Ziran] * 3
    #24465906 - 07/07/17 06:21 PM (6 years, 8 months ago)

Good tek,just want to add i find a thumb tack in the center of the cap makes it easier to pickup and move the caps without touching the foil.

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OfflineLogicaL ChaosM
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Re: Bod's proper spore printing TEK [Re: bodhisatta] * 2
    #24465916 - 07/07/17 06:25 PM (6 years, 8 months ago)

Now thats easy as fuck! :douchewink:

Whats the purpose of "tenting" the tinfoil?


--------------------
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OfflineCarmodycaramel
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Re: Bod's proper spore printing TEK [Re: LogicaL Chaos] * 3
    #24465919 - 07/07/17 06:27 PM (6 years, 8 months ago)

Quote:

LogicaL Chaos said:
Now thats easy as fuck! :douchewink:

Whats the purpose of "tenting" the tinfoil?





I believe its to keep the cap from drying because a dry cap wont drop spores.


--------------------
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OfflineLogicaL ChaosM
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Re: Bod's proper spore printing TEK [Re: Carmodycaramel] * 2
    #24465921 - 07/07/17 06:27 PM (6 years, 8 months ago)

Ah, i see.

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OfflineMr.Mushie402
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Re: Bod's proper spore printing TEK [Re: LogicaL Chaos] * 3
    #24465964 - 07/07/17 06:39 PM (6 years, 8 months ago)

Thanks bod. Will be making my first prints  in a few days. Now i dont even have to go searching for a good tek. Perfect timing:thumbup:

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InvisibleJust_A_Noob
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Re: Bod's proper spore printing TEK [Re: LogicaL Chaos] * 3
    #24465966 - 07/07/17 06:39 PM (6 years, 8 months ago)

looks professional  :takingnotes:


--------------------

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OfflineSteevo
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Re: Bod's proper spore printing TEK [Re: Just_A_Noob] * 2
    #24466350 - 07/07/17 09:42 PM (6 years, 8 months ago)

Nice :congrats:


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Offlinepotnug
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Re: Bod's proper spore printing TEK [Re: Steevo] * 2
    #24466601 - 07/08/17 12:04 AM (6 years, 8 months ago)

Good stuff, thanks Bod. :thumbup:

I suppose it would be beneficial to take a first print before trying to swab a cap too, in order to eliminate rogue mold spores and what not, what do you guys think? And what about something like PE that may not drop spores well, do you think you should set it in the sab a couple hours before swabbing?

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's proper spore printing TEK [Re: potnug] * 3
    #24466606 - 07/08/17 12:14 AM (6 years, 8 months ago)

With hard to print varieties or species sometimes you gotta do what you gotta do. You can try that technique and report back but swabbing gills is sometimes what has to happen to get spores from the stubborn ones

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Invisiblemustangbob3
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Re: Bod's proper spore printing TEK [Re: bodhisatta] * 3
    #24466806 - 07/08/17 04:36 AM (6 years, 8 months ago)

great old tek, not seen it mentioned for a while.
great write up about time someone wrote it up as a tek :thumbup:


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Offlinebill bixby
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Re: Bod's proper spore printing TEK [Re: mustangbob3] * 2
    #24466825 - 07/08/17 05:08 AM (6 years, 8 months ago)

sweet tek:thumbup:cheers

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Offlinebill bixby
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Re: Bod's proper spore printing TEK [Re: bill bixby] * 5
    #24493401 - 07/19/17 03:57 AM (6 years, 8 months ago)

hey Bodhi how long do you dry your prints for?

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Offlinemushpunx
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Re: Bod's proper spore printing TEK [Re: bill bixby] * 3
    #24493435 - 07/19/17 04:39 AM (6 years, 8 months ago)

This is a great write up.

Honestly it never occurred to me to double print, I've never heard of it. I don't usually make syringes so it was never much of an issue.

Learned something new


--------------------

Amateur Mycologists United
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OfflineAbeyance
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Re: Bod's proper spore printing TEK [Re: bodhisatta] * 2
    #24493437 - 07/19/17 04:40 AM (6 years, 8 months ago)

Awesome Bod! Thanks!!


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OfflineManifoldPrime
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Re: Bod's proper spore printing TEK [Re: Abeyance]
    #24493516 - 07/19/17 05:51 AM (6 years, 8 months ago)

Thanks bod I was just looking for a Printing Tek.

The last three times I tried to pring I got absolutely nothing. I think I harvested too early. How long after the veil breaks does it usually take for the cap to start sporulating? the veils of my current Texan batch broke yesterday.

:thaaannks:


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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Invisiblemorty422
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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24493684 - 07/19/17 08:07 AM (6 years, 8 months ago)

:asianofapproval:

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24493760 - 07/19/17 08:44 AM (6 years, 8 months ago)

Quote:

bill bixby said:
hey Bodhi how long do you dry your prints for?



Until they're dry. I just come back after a hour or two

Quote:

ManifoldPrime said:
Thanks bod I was just looking for a Printing Tek.

The last three times I tried to pring I got absolutely nothing. I think I harvested too early. How long after the veil breaks does it usually take for the cap to start sporulating? the veils of my current Texan batch broke yesterday.

:thaaannks:



Caps make spores before the veil breaks. The inside of the veil should be black with spores

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Offlinebill bixby
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24494563 - 07/19/17 04:48 PM (6 years, 8 months ago)

cheers. that's good to no
I've been leaving them for 12-24hrs.
all the gear no idea

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OfflineManifoldPrime
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24496400 - 07/20/17 01:48 PM (6 years, 8 months ago)

Quote:

Caps make spores before the veil breaks. The inside of the veil should be black with spores




My Texans dont sporulate but the B+ does. Ima try print tonight, with this tek. Will post results.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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OfflineManifoldPrime
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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24498121 - 07/21/17 07:43 AM (6 years, 8 months ago)

Quick update:

The B+ printed fine , 4 prints done so far I'm awaiting the second round batch. prints look like 6/10 quality. I have more than enough B+ myc and grains going, So I'll trade a few of these.

The Texans? not even a smidge of spores. The caps were open for 3 days, and the printing done over 12 hours. not a single spore by the looks of things. Is this a common issue? Is there a term for this? I got plenty of mycelium but I would like to reset the genetics. Can I take scrapings of the gills and put it to agar ?


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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InvisibleShroomway
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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24500433 - 07/22/17 09:05 AM (6 years, 8 months ago)

...just want to say I absolutely love this tek. I am very sure this will be my standard tek to use :smile:. I like the extra print step. Seems worth it to me! Thanks!

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24500438 - 07/22/17 09:07 AM (6 years, 8 months ago)

Quote:

ManifoldPrime said:
Quick update:

The B+ printed fine , 4 prints done so far I'm awaiting the second round batch. prints look like 6/10 quality. I have more than enough B+ myc and grains going, So I'll trade a few of these.

The Texans? not even a smidge of spores. The caps were open for 3 days, and the printing done over 12 hours. not a single spore by the looks of things. Is this a common issue? Is there a term for this? I got plenty of mycelium but I would like to reset the genetics. Can I take scrapings of the gills and put it to agar ?


Probably done making spores if they were open three days already

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OfflineManifoldPrime
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24500617 - 07/22/17 11:11 AM (6 years, 8 months ago)

They didnt seem to make a single spore. nothing on the caps or stems.


--------------------
My Magnum Opus:Thai Variety Comparison

Please correct me if I say something wrong!

| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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OfflineVoiceOfLogos


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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24515845 - 07/29/17 10:22 AM (6 years, 7 months ago)

Do you bring the roll of foil in the SAB to pull out the peices? I'm thinking if it's done outside the SAB shit could fall onto it?

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InvisiblebodhisattaMDiscordReddit
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Re: Bod's proper spore printing TEK [Re: VoiceOfLogos]
    #24515856 - 07/29/17 10:28 AM (6 years, 7 months ago)

The foil is in the sab

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Invisiblejkz
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24515940 - 07/29/17 10:55 AM (6 years, 7 months ago)

Did this the other day.
Used 5 fruits, ended up with 2 nice prints. Apparently 3 of them were sporeless.
This tek is so easy.
Makes printing a breeze.


--------------------
TEKs I like
Trade list (inc)


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OfflineManifoldPrime
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Re: Bod's proper spore printing TEK [Re: jkz]
    #24516261 - 07/29/17 02:00 PM (6 years, 7 months ago)

So sporeless is a thing. Its not just me. Phew

Edited by ManifoldPrime (07/29/17 02:00 PM)

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Invisiblejkz
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Re: Bod's proper spore printing TEK [Re: ManifoldPrime]
    #24516387 - 07/29/17 03:09 PM (6 years, 7 months ago)

Quote:

ManifoldPrime said:
So sporeless is a thing. Its not just me. Phew




Yeah. Caps were nice and flat too. Gills were creamy tan colour, the others were kinda dark. Tan caps dropped nothing I could see with the naked eye.

Good thing I grabbed one of the tan caps stems for a clone :wink:


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OfflinePsychedeliciouss
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24793747 - 11/19/17 01:50 PM (6 years, 4 months ago)

hello and thanks for the tek, at a point you say (loosely tent the prints with another piece of foil.) and also you have a picture for that. i may sound dump but i dont understand what i have to do at that point can u explain please.

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Re: Bod's proper spore printing TEK [Re: Psychedeliciouss]
    #24795063 - 11/20/17 07:36 AM (6 years, 4 months ago)

Hello, im new to this and have a question about spore prints (cant seem to understand how to make a completely new post lol)

I read that if you doing pf tek and let the mushroom break their veils they will shoot spores that will ruin future flushes? Is this true?

If so, how can i do a spore print without sacrificing upcoming flushes?
If i have one mushroom with a veil thats breaking(that is 8x bigger than the rest of the mushrooms), can i take that one and help it open the veil by cutting it and then make a spore print?

Cheers


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Re: Bod's proper spore printing TEK [Re: OericOss]
    #24795074 - 11/20/17 07:43 AM (6 years, 4 months ago)

Quote:

Psychedeliciouss said:
hello and thanks for the tek, at a point you say (loosely tent the prints with another piece of foil.) and also you have a picture for that. i may sound dump but i dont understand what i have to do at that point can u explain please.



Reread it maybe

Quote:

OericOss said:
Hello, im new to this and have a question about spore prints (cant seem to understand how to make a completely new post lol)

I read that if you doing pf tek and let the mushroom break their veils they will shoot spores that will ruin future flushes? Is this true?

If so, how can i do a spore print without sacrificing upcoming flushes?
If i have one mushroom with a veil thats breaking(that is 8x bigger than the rest of the mushrooms), can i take that one and help it open the veil by cutting it and then make a spore print?

Cheers



Yep mushrooms make spores...
You harvest the mushrooms before they shoot spores all over your cakes. Spores Don't stop the next flush

You can do whatever you want

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24864708 - 12/23/17 08:27 PM (6 years, 3 months ago)

Cheers for this TEK, Bod! My n00b question is in between the first and second printing, do you respray the inside of the SAB with anything like the warm water/soap mixture or 70% ISO? Thanks in advance!


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Re: Bod's proper spore printing TEK [Re: RagonkTerramy]
    #24864711 - 12/23/17 08:29 PM (6 years, 3 months ago)

No. Respraying only would help stir stuff up imo

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OfflineRagonkTerramy
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24864779 - 12/23/17 09:34 PM (6 years, 3 months ago)

Quote:

bodhisatta said:
No. Respraying only would help stir stuff up imo




That's what I figured but I wanted to make sure. :thanx:


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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #24938525 - 01/24/18 11:30 AM (6 years, 2 months ago)

Boom! Simple and makes sense, clean and easy, gonna print the next batch, no need to chase down spores again.

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Offlineomegaman
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Re: Bod's proper spore printing TEK [Re: mustangbob3]
    #25063541 - 03/14/18 05:31 PM (6 years, 16 days ago)

veils just broke gills are really pale right now...they are albino a+ when should i take a print.

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Re: Bod's proper spore printing TEK [Re: omegaman]
    #25063572 - 03/14/18 05:48 PM (6 years, 16 days ago)

When it starts to make spores. They'll be purple/black

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Offlineomegaman
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25063577 - 03/14/18 05:50 PM (6 years, 16 days ago)

thank you brother!

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25264784 - 06/13/18 01:11 AM (5 years, 9 months ago)

Very well written and clear. Thanks for the info!

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Re: Bod's proper spore printing TEK [Re: Vargus0]
    #25309491 - 07/05/18 09:06 AM (5 years, 8 months ago)

Fantastic as always. How long do spore prints keep in the fridge?

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Re: Bod's proper spore printing TEK [Re: watermelonman]
    #25309524 - 07/05/18 09:36 AM (5 years, 8 months ago)

Beats me, never put a print in the fridge before. They last a decade in a drawer

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25311431 - 07/06/18 10:15 AM (5 years, 8 months ago)

some great info here, thanks!

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Re: Bod's proper spore printing TEK [Re: dickfartmcpoopus]
    #25311619 - 07/06/18 12:07 PM (5 years, 8 months ago)

Thank you for this :smile:

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25328349 - 07/15/18 06:08 PM (5 years, 8 months ago)

:thumbup:

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Re: Bod's proper spore printing TEK [Re: BatchScript]
    #25344248 - 07/24/18 11:55 AM (5 years, 8 months ago)

These TEKs are saving my ass! Very grateful to you Bod!  :bow2:

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Re: Bod's proper spore printing TEK [Re: Nosheeple]
    #25383320 - 08/12/18 10:30 AM (5 years, 7 months ago)

Using caps with newly torn veils, a 5 hours wait created decent first prints.  But 22 hours later, none of 10 caps left any secondary print.

Anyone else experience this?  (Weather here is humid and balmy.)

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25404924 - 08/22/18 09:55 AM (5 years, 7 months ago)

Very good information. Thank you Bod, again.

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Re: Bod's proper spore printing TEK [Re: drprobablyno]
    #25432692 - 09/03/18 10:40 AM (5 years, 6 months ago)

Very informative, thank you!


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Re: Bod's proper spore printing TEK [Re: gizmodo] * 1
    #25445164 - 09/08/18 01:23 PM (5 years, 6 months ago)

Thanks Bod for this easy step by step way to make spore prints.  :jackiechanofapproval:


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Re: Bod's proper spore printing TEK [Re: Luko]
    #25445216 - 09/08/18 02:02 PM (5 years, 6 months ago)

:takingnotes:


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Re: Bod's proper spore printing TEK [Re: Johnny.rotten]
    #25445329 - 09/08/18 02:52 PM (5 years, 6 months ago)

Thanks! very useful and clear  :takingnotes:


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Re: Bod's proper spore printing TEK [Re: ElViajero]
    #25508717 - 10/03/18 10:12 AM (5 years, 5 months ago)

By tent it simply means to fold foil in a triangular shape to be laid over the prints?

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Re: Bod's proper spore printing TEK [Re: Psychonautt] * 1
    #25508733 - 10/03/18 10:17 AM (5 years, 5 months ago)

Yep



My picture that bad ? Lol

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25508881 - 10/03/18 11:05 AM (5 years, 5 months ago)



--------------------
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Re: Bod's proper spore printing TEK [Re: JHOVA]
    #25508895 - 10/03/18 11:11 AM (5 years, 5 months ago)

:rasta: man i still dont get the picture
i dont think your edit did it better.... or am i just too high?
:smokinacandycane:


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Re: Bod's proper spore printing TEK [Re: ElViajero]
    #25508904 - 10/03/18 11:13 AM (5 years, 5 months ago)

Pretend you are Looking through a tunnel of aluminum shaped like a tent.:shrug:


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Re: Bod's proper spore printing TEK [Re: JHOVA]
    #25509130 - 10/03/18 12:31 PM (5 years, 5 months ago)

I just want to be 100% clear before I dump a couple hundred in all the bells and whistles to take my shitty PF Tek up to Bodh Tek. I told myself before I checkout on Amazon I want every last step so understood and memorized I can recite them as if I've done them before. I wrote down anything that was unclear then searched around and now it's question time. There will be a couple more tomorrow in other Bodh Tek threads.

Thanks

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25519858 - 10/07/18 02:52 PM (5 years, 5 months ago)

G.O.A.T.

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Re: Bod's proper spore printing TEK [Re: ElViajero] * 1
    #25599261 - 11/07/18 05:36 PM (5 years, 4 months ago)

Quote:

ElViajero said:
:rasta: man i still dont get the picture
i dont think your edit did it better.... or am i just too high?
:smokinacandycane:




Honestly confused AF about the tent thing too (and I'm not that high) :confused:

Bod...do you mean a flat, folded triangle/tent, or a container-type tent with air-volume over the print like a pyramid?

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Re: Bod's proper spore printing TEK [Re: Kodanama]
    #25606515 - 11/11/18 04:25 AM (5 years, 4 months ago)

Great Tek Bod. I'll try this. 👍🏻

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Re: Bod's proper spore printing TEK [Re: gaia-madre]
    #25623900 - 11/18/18 06:44 PM (5 years, 4 months ago)

Trying this tonight for the first time, wish me luck! :smile:

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Re: Bod's proper spore printing TEK [Re: captmarc]
    #25661997 - 12/06/18 03:44 PM (5 years, 3 months ago)

Thanks Bod!

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Re: Bod's proper spore printing TEK [Re: whitewombat]
    #25678543 - 12/14/18 09:38 AM (5 years, 3 months ago)

I have made a spore print a few days ago before reading this post.
WOndering if I should just toss it, as I didn't use my SAB or any sterile technique. Just did the spore print the way I do it for identification...

Is it worth keeping it or will the attempt fail for certain?

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Re: Bod's proper spore printing TEK [Re: Time Tourist]
    #25678611 - 12/14/18 10:09 AM (5 years, 3 months ago)

It's probly dirty but u should be able to start it on agar and clean it up. Or if u have other shrooms growing make a cleaner print.

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Re: Bod's proper spore printing TEK [Re: nube424]
    #25678638 - 12/14/18 10:25 AM (5 years, 3 months ago)

Yeah was planning to use other mushrooms as well.
But was curious if this was going to be useless or not... Might be worth it. Am a beginner with agar so it might be a good test.

Is it okay to still dry the used cap and store it for ingestion later?

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Re: Bod's proper spore printing TEK [Re: Time Tourist]
    #25678641 - 12/14/18 10:25 AM (5 years, 3 months ago)

Yes.

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Re: Bod's proper spore printing TEK [Re: nube424]
    #25741140 - 01/13/19 01:20 AM (5 years, 2 months ago)

By sound of this tek, the second print is more sterile which would be good for syringes, but first print, good for agar? Since first print may drop most contams, is this correct?

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Re: Bod's proper spore printing TEK [Re: woofwoof]
    #25741288 - 01/13/19 05:39 AM (5 years, 2 months ago)

2nd print should be cleaner yes. But if u wanna make syringes from a print ur sterile techniques gotta be ON POINT or u will get bacteria.

I only fuck with agar... but I know some people like spore syringes, so yes u would have better luck with the second print.

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Re: Bod's proper spore printing TEK [Re: woofwoof]
    #25741438 - 01/13/19 07:50 AM (5 years, 2 months ago)

In theory yes that's the idea sounds like you read the tek

99.9% of syringes are made with the the first print because 99.9% of people only take one print. They work fine.

Vendors buy prints from lots of people sometimes they're not even TCs.

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25741445 - 01/13/19 07:55 AM (5 years, 2 months ago)

Vendors are just some dude buying spore prints and diluting them in syringes?

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Re: Bod's proper spore printing TEK [Re: Failboat]
    #25741461 - 01/13/19 08:03 AM (5 years, 2 months ago)

Yes. Well a couple places grow in legal countries but still heavily rely on bought prints too.

Really just living off the fact noobs are too paranoid to do something so easy themselves

Even with an average spore syringe you can still go straight to grain and get like 5-10 out of 10 jars good

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Offlineqiffrox
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #25761130 - 01/22/19 03:15 PM (5 years, 2 months ago)

thanks for that, very useful stuff

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Re: Bod's proper spore printing TEK [Re: qiffrox]
    #25874291 - 03/14/19 04:55 PM (5 years, 16 days ago)

Awesome tek, I will be trying this soon with a syringe. I would also like to try agar. More research for that. Plus I'm using spawn bags. Hopefully it goes well. Thanks for the info. It is much appreciated. :rocket:


--------------------
:rockon::mushroom2::rockon:

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26097701 - 07/09/19 08:42 AM (4 years, 8 months ago)

:takingnotes: These TEKs are amazing! Thank you for sharing your knowledge with us!!

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26109278 - 07/15/19 08:55 AM (4 years, 8 months ago)

Good write-up!

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OfflineSilver_Peaks
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Re: Bod's proper spore printing TEK [Re: shroomanly]
    #26117538 - 07/19/19 08:56 AM (4 years, 8 months ago)

When following this TEK is it worthwhile to dry the spent caps use them as normal, or will they have lost potency compared to a cap that wasn't used to make a spore print?

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Re: Bod's proper spore printing TEK [Re: Silver_Peaks]
    #26117617 - 07/19/19 09:56 AM (4 years, 8 months ago)

Ive always ate them or dried them up

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26126307 - 08/08/19 12:36 AM (4 years, 7 months ago)

Does this method require placing an empty mason jar over top of the caps? Would boiling empty 1/2 pint mason jars for 10 minutes be adequate for sterilization? TIA

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Re: Bod's proper spore printing TEK [Re: Spudz92]
    #26126412 - 08/08/19 04:46 AM (4 years, 7 months ago)

Requires everything that the first post shows and talks about. Idk where you got the mason jar idea from

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26294371 - 11/02/19 02:54 PM (4 years, 4 months ago)

Will you be doing any teks or videos specifically on cloning? What would seem as a better idea to you to get started? Gambling on a multispore to grain going well and cloning the best looking fruit? Or going spores to agar and isolating one strain and hoping it fruits ok?


--------------------

Edited by DeckardCain (11/02/19 02:54 PM)

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OfflineImpatientwonderer
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Re: Bod's proper spore printing TEK [Re: Carmodycaramel]
    #26368419 - 12/08/19 07:00 AM (4 years, 3 months ago)

Such dark prints! Nice Bod!

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26413705 - 01/03/20 06:21 AM (4 years, 2 months ago)

Wondering if its best practice to take a spore print of a 2nd flush? Would a second flush spore print be any different from the first flush as far as passing on genetics? Would the second flush spore print pass on genetics that lean toward slow growth?

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Re: Bod's proper spore printing TEK [Re: markclark]
    #26413786 - 01/03/20 07:44 AM (4 years, 2 months ago)

Prints basically reset genetics. You can weed out traits by carefully taking prints, but i wouldn't worry about where you're taking prints from so much unless you're trying to stabilize wild genetics

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26413937 - 01/03/20 09:21 AM (4 years, 2 months ago)

Ok cool. Thanks for this great tek and help. I had an awesome 1st flush with some GT, but didn't take prints. It sounds like I am good to go for taking prints with second flush though. :thumbup:

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Re: Bod's proper spore printing TEK [Re: markclark]
    #26445877 - 01/22/20 09:03 AM (4 years, 2 months ago)

I get perfect prints using this method!


--------------------
It's not so much that I am the center of the universe, it's that the universe revolves around me...




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Re: Bod's proper spore printing TEK [Re: Johnny51v3]
    #26447901 - 01/23/20 09:55 AM (4 years, 2 months ago)

great information thank you so much for your time and help

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Re: Bod's proper spore printing TEK [Re: Kodanama]
    #26495414 - 02/20/20 03:09 PM (4 years, 1 month ago)

Quote:

Kodanama said:
Quote:

ElViajero said:
:rasta: man i still dont get the picture
i dont think your edit did it better.... or am i just too high?
:smokinacandycane:




Honestly confused AF about the tent thing too (and I'm not that high) :confused:

Bod...do you mean a flat, folded triangle/tent, or a container-type tent with air-volume over the print like a pyramid?




Bod, I got the same questions as these dudes.  I don't see the answer or maybe miss it,  but I am so confused as them.  What kind of tent mane???

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OfflineEbilPhish
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Re: Bod's proper spore printing TEK [Re: PNat]
    #26495661 - 02/20/20 05:12 PM (4 years, 1 month ago)

👍

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26527943 - 03/10/20 06:30 PM (4 years, 19 days ago)

Bod your teks are fucking great man! Appreciate your hard work.

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OfflineLSA Woodrose
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Re: Bod's proper spore printing TEK [Re: BuzzAdvil]
    #26584539 - 04/07/20 05:38 PM (3 years, 11 months ago)

Amazing information! All right, I am about to ask what I can safely assume has to be the dumbest question in the thread, but here goes. I am going into the fruiting stage of my first grow. This is my thread [here] in case you're curious lol. 24 BRF Cakes, ala PF Tek and two Sterilite boxes I made into SGFCs. Anyway, birthined the final 4 cakes today, so as of tomorrow, after dunk & roll, all cakes will be in their fruiting homes.

As an aside, the reason I am in this thread is I was directed here from my thread. Since I am getting some serious fruits, though not yet ready to harvest, it was recommended to me by several awesome guys from the forum that I check out this thread for creating MS syringes, rather than going to the site sponsor to buy them like I did for this grow.

tl;dr - Sorry about the build up. But my question is, when you are making the prints, do you lose those big caps for consumption? Or do you just take slices, or whatever from them and then can eat the large caps later on?

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OfflineA.k.aM
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Re: Bod's proper spore printing TEK [Re: LSA Woodrose] * 1
    #26584770 - 04/07/20 07:16 PM (3 years, 11 months ago)

After you’re done printing the caps you just throw them in the dehydrator and they’re good to go.


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OfflineWhoa_Brah
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26934759 - 09/14/20 09:08 AM (3 years, 6 months ago)

Awesome TEK! You make life easy for us newbs with this stuff, :grin:

I've read in a few spots that it's recommended to put a drop of water on the top of the cap to encourage sporulation. Have you ever tried that? Any thoughts on whether or not it's advisable?

Cheers


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Re: Bod's proper spore printing TEK [Re: Whoa_Brah]
    #26934938 - 09/14/20 10:32 AM (3 years, 6 months ago)

I don’t think that’s necessary for cubes.

Stuff like tamps that have tiny caps and dry out quickly is where I’ve seen this done.


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OfflinePhony Phone

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Re: Bod's proper spore printing TEK [Re: A.k.a]
    #26970477 - 10/05/20 11:46 AM (3 years, 5 months ago)

Do you unroll the foil inside the sab?
How does one do it if their sab isnt that big?

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Re: Bod's proper spore printing TEK [Re: Phony Phone]
    #26983739 - 10/13/20 05:19 PM (3 years, 5 months ago)

how do you leave yur prints to dry? sab holes open? would sealing the sab holes with saran wrap hasten the evaporation?

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Re: Bod's proper spore printing TEK [Re: Phony Phone]
    #26983745 - 10/13/20 05:21 PM (3 years, 5 months ago)

Yea holes open. With also no air movement in the room I always felt good about it.

Also my home is usually dry as fuck for 9 months of the year

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #26983747 - 10/13/20 05:26 PM (3 years, 5 months ago)

I just followed these steps and it went real smooth. Thanks for sharing

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Re: Bod's proper spore printing TEK [Re: nogoodnamesleft]
    #26983879 - 10/13/20 06:45 PM (3 years, 5 months ago)

Pitt a towel over the holes. Should be fine.


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Offlineenzofilo
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Re: Bod's proper spore printing TEK [Re: PNat]
    #27058558 - 11/26/20 11:11 AM (3 years, 3 months ago)

Quote:

PNat said:
Quote:

Kodanama said:
Quote:

ElViajero said:
:rasta: man i still dont get the picture
i dont think your edit did it better.... or am i just too high?
:smokinacandycane:




Honestly confused AF about the tent thing too (and I'm not that high) :confused:

Bod...do you mean a flat, folded triangle/tent, or a container-type tent with air-volume over the print like a pyramid?




Bod, I got the same questions as these dudes.  I don't see the answer or maybe miss it,  but I am so confused as them.  What kind of tent mane???




Same here. English is not my first language, so I'm guessing he means cover??:confused:

Anyway, thanks for the tek bod!

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Re: Bod's proper spore printing TEK [Re: enzofilo] * 1
    #27058586 - 11/26/20 11:22 AM (3 years, 3 months ago)

Like a triangle with foil.

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OfflinePhony Phone

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27058603 - 11/26/20 11:31 AM (3 years, 3 months ago)

Ended up just saran wrapping the holes and folding them upwards a bit to leave small opening for aeration.

Works fine! And yeah, no drafts in the room or else you are looking as some dusty friends partying on your prints.  :harassment:

Quote:

enzofilo said:
Quote:

PNat said:
Quote:

Kodanama said:
Quote:

ElViajero said:
:rasta: man i still dont get the picture
i dont think your edit did it better.... or am i just too high?
:smokinacandycane:




Honestly confused AF about the tent thing too (and I'm not that high) :confused:

Bod...do you mean a flat, folded triangle/tent, or a container-type tent with air-volume over the print like a pyramid?




Bod, I got the same questions as these dudes.  I don't see the answer or maybe miss it,  but I am so confused as them.  What kind of tent mane???




Same here. English is not my first language, so I'm guessing he means cover??:confused:

Anyway, thanks for the tek bod!





Interpreted it as fold a piece of foil over the foil your spores drop on and have it act as a tent.
Well it worked. :cool:

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Offlinejoy-of-shrooms
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27184219 - 02/03/21 09:42 AM (3 years, 1 month ago)

Thanks!

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Re: Bod's proper spore printing TEK [Re: joy-of-shrooms]
    #27242729 - 03/08/21 06:24 AM (3 years, 21 days ago)

I'm excited about to do my first prints...
On the 4th flush however, missed my previous opportunities.

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Re: Bod's proper spore printing TEK [Re: riomar]
    #27245949 - 03/10/21 01:21 AM (3 years, 20 days ago)

Got nice 1st row prints...
Waiting for the second.
Thx!

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Re: Bod's proper spore printing TEK [Re: riomar]
    #27245951 - 03/10/21 01:22 AM (3 years, 20 days ago)

by the way...
I used some empty small pp5 (the ones for agar) as "tent"

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Offlinegabbk
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Re: Bod's proper spore printing TEK [Re: riomar]
    #27273835 - 03/28/21 11:32 PM (3 years, 1 day ago)

Is it viable to cut caps and store them in the fridge to print later? let's say a day or two later?


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OfflineJohnS0N
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27592156 - 12/23/21 05:45 PM (2 years, 3 months ago)

Superb guide as usual from Bod. Cheers.

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Offlinevoodoviking
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Re: Bod's proper spore printing TEK [Re: JohnS0N]
    #27597489 - 12/28/21 02:29 PM (2 years, 2 months ago)

Thank you very much for sharing your knowledge and for posting a very beginner friendly description.

Is there an indication of the mushrooms state of growth where you exactly  can define when it is time to pick and harvest it spores. Every shroom can naturally defer from each other and unpredictable of the environment.

I guess better be to early than to late- Or the spores are all over in the container.

I heard that the mushrooms are more potential before they start to grow into the state of developing spores. 

If you are not able to be home and waiting for the moment. Is there another way of saving the spore harvesting ?

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Invisiblegoodcleanfun
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27603510 - 01/02/22 09:46 AM (2 years, 2 months ago)

Bad ass. Hoping to be using this tek here shortly.

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Offlinedreamwever
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27750866 - 04/25/22 03:53 PM (1 year, 10 months ago)

Hello Bob,
Thank you for the spore print instructions.


In short, westest summer autumn for awhile here in Australia, usually my area is abundant with cubensis, but this year has been bad for fungi, my only reason i think its so, as we have just come out of the worst drought in our area, and somehow the myc maybe retreated in the dry.

Finally went on a big trip to try to get spore prints found a few cubes, but only in small random spots, though the location would really go off in a good season.





I got home, i left the best cubes in the fridge. Sterlized the stovetop and razer, used gloves etc. Should have used the better shroom for the spore print, hopefully i can still use it for a print, as its cap wasnt as dark as the others.

How do i store the prints? i  have ziplock bags and mylar foil, currently in the pantry in sterlized containers.

How do you know when they are dry enough to store?

I have to read up on cultivation before proceeding.

THank you :smile:

Edit - update - prints now n foil in wardrobe. I tried to be as sterile as possible without a SAB to fold the prints with tweezers heated with jet ligher within the container and immediately into ziplock, fingers crossed not too much contam from the wild spores

Edited by dreamwever (04/26/22 10:48 PM)

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Offlinezak69
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27905352 - 08/16/22 08:42 AM (1 year, 7 months ago)

I wish I'd seen this earlier...
Made a spore print on foil and left in my desk draw for 24 hours.
Not sterile at all...
I'm guessing it's probably contaminated

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #27952643 - 09/16/22 10:36 AM (1 year, 6 months ago)

thanks

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Re: Bod's proper spore printing TEK [Re: thecrazyshroom]
    #27953422 - 09/16/22 07:01 PM (1 year, 6 months ago)

Quote:

thecrazyshroom said:
thanks



Noticing a trend here....everything good man?


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OfflineBoySage
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Re: Bod's proper spore printing TEK [Re: Tormato]
    #27953470 - 09/16/22 07:23 PM (1 year, 6 months ago)

Quote:

Tormato said:
Quote:

thecrazyshroom said:
thanks



Noticing a trend here....everything good man?



Have you seen Paul Stamets tell his Amanita pantherina story on JRE? Kinda reminded me of that :awesomenod:


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Appreciate all the input I can get. Willing to ask (possibly annoying) questions, but also good at taking shit.

Thanks fam!

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OfflineItsfullofstars2
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Re: Bod's proper spore printing TEK [Re: Carmodycaramel]
    #28217727 - 03/06/23 05:15 PM (1 year, 23 days ago)

Tent cover to protect from accidental contamination?

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OfflineItsfullofstars2
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Re: Bod's proper spore printing TEK [Re: Itsfullofstars2]
    #28217785 - 03/06/23 06:04 PM (1 year, 23 days ago)

Doesn’t seem like a tend would prevent dehydration much?

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #28302647 - 05/01/23 07:38 PM (10 months, 22 days ago)

Seems every tek I end up using as my favorite is yours. good job

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Offlinesocalchef00
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #28355398 - 06/11/23 09:55 AM (9 months, 12 days ago)

Damn. awesome way to do it. I should of read this before I made my prints.
Hopefully I didn't contaminate mine.

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Invisibleiomoth
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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #28495416 - 10/07/23 12:09 PM (5 months, 18 days ago)

many thanks

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Re: Bod's proper spore printing TEK [Re: bodhisatta]
    #28549645 - 11/19/23 09:35 PM (4 months, 6 days ago)

Favoriting thread for later, thanks for the info.

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