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OfflinePsychedelicScience
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Grocery Store Agar Tek question.
    #24427859 - 06/23/17 12:14 AM (3 years, 10 months ago)

I was reading this tek and I noticed he stated he would sterilize a needle with water and squirt water into the agar dish and suck it back up to make a Liquid inoculate?
Who has any experience making LC's this way?


Edited by PsychedelicScience (06/23/17 01:06 AM)


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Offlineichugwindex
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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24427905 - 06/23/17 12:36 AM (3 years, 10 months ago)

Search "liquid inoculant" and behold


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OfflinePsychedelicScience
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Re: Grocery Store Agar Tek question. [Re: ichugwindex]
    #24427964 - 06/23/17 01:06 AM (3 years, 10 months ago)

Quote:

ichugwindex said:
Search "liquid inoculant" and behold



Nothing I found answered my question...


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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24427981 - 06/23/17 01:11 AM (3 years, 10 months ago)

Quote:

PsychedelicScience said:

Who has any experience making LC's this way?





i dont think anybody does because nobody would do that. why WOULD you need to make LC like that?


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OfflinePsychedelicScience
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Re: Grocery Store Agar Tek question. [Re: mushboy]
    #24428003 - 06/23/17 01:22 AM (3 years, 10 months ago)

Quote:

mushboy said:
Quote:

PsychedelicScience said:

Who has any experience making LC's this way?





i dont think anybody does because nobody would do that. why WOULD you need to make LC like that?



Umm because some people follow the tek and have clones and isolates they want to make into a LC plus going straight to grains. It seems a lot more sterile then taking a piece and dropping it into a LC solution IMO is why I'm interested.


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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience] * 1
    #24428085 - 06/23/17 02:13 AM (3 years, 10 months ago)

That's kind of more like LI look up liquid inoculant
https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&how=all&words=Liquid+inoculant&newerval=4&newertype=y&minwords=200&limit=25&forum=f2&showmain=1&image=1&sort=r&way=d
Click the 2nd and 4th link

If you really want to make a LC putting a wedge from an agar dish into a Lc jar is the best way. If your sterile technique isn't up to par enough to feel comfortable and get reliable results doing It that way then you don't belong doing LC work.


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Re: Grocery Store Agar Tek question. [Re: bodhisatta]
    #24428095 - 06/23/17 02:20 AM (3 years, 10 months ago)

:whathesaid:

might find my agar guide informative as well


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Re: Grocery Store Agar Tek question. [Re: c10h12n2o]
    #24428300 - 06/23/17 05:53 AM (3 years, 10 months ago)

https://www.shroomery.org/forums/showflat.php/Number/23740857 pasty does that on his smaller agar jars.... but thats only for a small amount of jars that link bodhi posted has anything and everything on it you need to make some good inoculants


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OfflinePsychedelicScience
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Re: Grocery Store Agar Tek question. [Re: bodhisatta]
    #24430464 - 06/24/17 12:39 AM (3 years, 10 months ago)

Quote:

bodhisatta said:
That's kind of more like LI look up liquid inoculant
https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&how=all&words=Liquid+inoculant&newerval=4&newertype=y&minwords=200&limit=25&forum=f2&showmain=1&image=1&sort=r&way=d
Click the 2nd and 4th link

If you really want to make a LC putting a wedge from an agar dish into a Lc jar is the best way. If your sterile technique isn't up to par enough to feel comfortable and get reliable results doing It that way then you don't belong doing LC work.



Nobody said "my sterile technique isn't up to par" and I'm looking for a easy way out!
I'm simply asking who has experience with this! If you don't have any experience then please save your arrogance.


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OfflinePsychedelicScience
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Re: Grocery Store Agar Tek question. [Re: c10h12n2o]
    #24430470 - 06/24/17 12:41 AM (3 years, 10 months ago)

Quote:

c10h12n2o said:
:whathesaid:

might find my agar guide informative as well



How about you guys learn how to read at the same time. I never said I don't know how to do agar! Iv done 100+ transfers not counting G2G! Why is it so hard asking a simple question here nowadays. No wonder why this site is dead.


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OfflinePsychedelicScience
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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24430481 - 06/24/17 12:45 AM (3 years, 10 months ago)

The reason I ask this is because it seems very redundant to isolate a strain for a month to then have to wait another week or more for the LC to colonize. Have any of you actually tried the method I'm asking about?


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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24430484 - 06/24/17 12:48 AM (3 years, 10 months ago)

No need to get all crazy haha ive made liquid inoculant to Lc which helps speed things up but no matter what you decide on doing its going to take atleast 10+ days for the lc to be done unless youre only using 1 or 200ml of lc


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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience] * 1
    #24430491 - 06/24/17 12:48 AM (3 years, 10 months ago)

Quote:

PsychedelicScience said:
I was reading this tek and I noticed he stated he would sterilize a needle with water and squirt water into the agar dish and suck it back up to make a Liquid inoculate?
Who has any experience making LC's this way?




I did that back before I had an account here. It works but is a bit clumsy. I refined his method a bit. Someone posted the link to it above.

Don't get salty, this place is hardly dead. There are 1000's of posts a day. But people often think LC is a way to bypass agar which is why people were trying to steer you to it. It's not that they are trying to bust your balls, their intent is to help.


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OfflinePsychedelicScience
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Re: Grocery Store Agar Tek question. [Re: Pastywhyte]
    #24430527 - 06/24/17 01:04 AM (3 years, 10 months ago)

Quote:

Pastywhyte said:
Quote:

PsychedelicScience said:
I was reading this tek and I noticed he stated he would sterilize a needle with water and squirt water into the agar dish and suck it back up to make a Liquid inoculate?
Who has any experience making LC's this way?




I did that back before I had an account here. It works but is a bit clumsy. I refined his method a bit. Someone posted the link to it above.

Don't get salty, this place is hardly dead. There are 1000's of posts a day. But people often think LC is a way to bypass agar which is why people were trying to steer you to it. It's not that they are trying to bust your balls, their intent is to help.



Thanks a lot brother!


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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24430610 - 06/24/17 01:43 AM (3 years, 10 months ago)

I have done it and don't like it personally. I would recommend cutting a wedge and putting it in a jar of water and making LI that way. LI and LC are not the same, btw. Are you asking if you can make an LC from this type of LI? Of course you could, but again, i'd recommend just putting a wedge directly into your nutrient solution.


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Re: Grocery Store Agar Tek question. [Re: Boromyc]
    #24430771 - 06/24/17 04:01 AM (3 years, 10 months ago)

Why drop a whole wedge into nutrient broth when you can easily scrape mycelium off the agar surface and transfer?


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Re: Grocery Store Agar Tek question. [Re: Morelman]
    #24430778 - 06/24/17 04:08 AM (3 years, 10 months ago)

I drop a whole plate in id figure it will colonize faster


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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24430789 - 06/24/17 04:29 AM (3 years, 10 months ago)

Quote:

PsychedelicScience said:
Quote:

ichugwindex said:
Search "liquid inoculant" and behold



Nothing I found answered my question...




Quote:

PsychedelicScience said:
I was reading this tek and I noticed he stated he would sterilize a needle with water and squirt water into the agar dish and suck it back up to make a Liquid inoculate?
Who has any experience making LC's this way?




thats a Liquid Inoculate. A LC is a liquid culture and is a lot more nutrient rich than an LI. and LI uses sterile water and an agar plate to distribute mycelium mass. LC uses a broth to grow out more mycelium in a nutrient rich environment that is liquid. Much like an agar plate, except with a clean liquid broths. (IE honey, karo mix)


Edited by Ziran (06/24/17 04:35 AM)


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InvisibleMunchauzen
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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24431007 - 06/24/17 09:27 AM (3 years, 10 months ago)

Quote:

PsychedelicScience said:
I was reading this tek and I noticed he stated he would sterilize a needle with water and squirt water into the agar dish and suck it back up to make a Liquid inoculate?
Who has any experience making LC's this way?



i made this tek video some years ago, and i typically dont share it. but since u asked...



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InvisiblebodhisattaM
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Re: Grocery Store Agar Tek question. [Re: PsychedelicScience]
    #24431087 - 06/24/17 10:22 AM (3 years, 10 months ago)

Quote:

PsychedelicScience said:
Quote:

bodhisatta said:
That's kind of more like LI look up liquid inoculant
https://www.shroomery.org/forums/dosearch.php?where=body&tosearch=main&how=all&words=Liquid+inoculant&newerval=4&newertype=y&minwords=200&limit=25&forum=f2&showmain=1&image=1&sort=r&way=d
Click the 2nd and 4th link

If you really want to make a LC putting a wedge from an agar dish into a Lc jar is the best way. If your sterile technique isn't up to par enough to feel comfortable and get reliable results doing It that way then you don't belong doing LC work.



Nobody said "my sterile technique isn't up to par" and I'm looking for a easy way out!
I'm simply asking who has experience with this! If you don't have any experience then please save your arrogance.



:facepalm:


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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