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Invisibledowodenum
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: Hysteria]
    #28218193 - 03/06/23 10:34 PM (10 months, 17 days ago)

Start PF from your prints. Use up to half the syringe on the first batch and try to get fruits to clone to agar, for LI on the next batch. Keep the half syringe as backup.

If you want to be extra safe, take spores to agar before even making a syringe!


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Invisibledowodenum
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: Hysteria]
    #28218526 - 03/07/23 07:24 AM (10 months, 17 days ago)

Does the glossary say PK tek?? That's really bad lol

I've thought it could use some updating too. The acronyms list is missing some things like MPT (micropore tape)

Updated PF tek guide


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Invisibledowodenum
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: Hysteria]
    #28218576 - 03/07/23 08:16 AM (10 months, 16 days ago)

Yeah PF tek. Don't worry, you'll learn via context alone, eventually.


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Invisibledowodenum
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: Hysteria]
    #28218606 - 03/07/23 09:19 AM (10 months, 16 days ago)

I do yeah, 15+ year old stuff lol. Ran out recently, got some Royal Blue gel from Ann Clark on the way.


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Invisibledowodenum
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Re: [STICKY] ⚠ Where to Start Flow Chart, QnA, and helpful links ⚠ [Re: Hysteria]
    #28218712 - 03/07/23 10:27 AM (10 months, 16 days ago)

I think it's darker than what I had, but just use less.


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Re: flowchart all in one [Re: Way]
    #28270294 - 04/09/23 06:26 PM (9 months, 14 days ago)

There are water chiller units with a pump built-in that you can run on a cycle timer. A coil of flexible tubing in a TIT incubator might work?


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