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InvisibleprettyMushy
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Registered: 04/03/12
Posts: 1,828
Loc: Canada
Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: MushrooMom]
    #24703807 - 10/12/17 12:59 AM (6 years, 3 months ago)

great info, why is grain spawn not recommended? Thats the only kind i have done and had no problems? lol.. rye to manure


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InvisibleprettyMushy
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: PreparationH]
    #24703857 - 10/12/17 02:08 AM (6 years, 3 months ago)

Quote:

PreparationH said:
It's not recommended to inoculate your grains with spores. spores/syringe may have contams.  I've gotten away with it too but you're much better off germinating your spores on agar then inoculating with a wedge into your grains.





is it because of the colonization time from spore to active colony on the grain? Im guessing brown rice will take a foothold a lot quicker than on grain therefore giving the colony a better chance at life against contams? i dunno, ive always done pressure cooked grain to manure never really thought about it or had problems.


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InvisibleprettyMushy
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: bodhisatta]
    #24704280 - 10/12/17 08:52 AM (6 years, 3 months ago)

Quote:

bodhisatta said:

No its because spore syringes are not really clean enough to inoculate anything but cakes or agar with.

Cakes don't get shaken and agar can separate the nasty from the good. With agar you get clean mycelium for inoculation of grain





thats crazy because thats the only way ive ever done it :shrug:


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InvisibleprettyMushy
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Re: Where to Start Flow Chart and helpful links also ask your questions here Q&A [Re: bodhisatta]
    #24704391 - 10/12/17 09:52 AM (6 years, 3 months ago)

Quote:

bodhisatta said:

Well it can work sure. Ive done grows with syringes too.

Quote:

bodhisatta said:
if you make a LC with a spore syringe or spores in general just assume it's going to be contaminated and fail. unless you test it and it works, in which case you got lucky.

LC needs to be started with a clean culture. Like on agar.

A single drop of spore syringe solution can have literally millions of spores
https://www.shroomery.org/forums/showflat.php/Number/21365919/fpart/all/vc/1

the chances of 1 contaminant being part of 1,000,000 cube spores is pretty high, we grow cubes out in the open in fruiting chambers. You would have to grow the shrooms in a bio-hazard clean room to get a print to be clean enough to make reliable LC with.

LC is liquid so one single contamination will and homogenize so that the entire LC will contain the contaminant microorganism.

on agar a single contaminant or two stays localized on the first dish and you can transfer clean mycelium to a new dish leaving any contamination away. this clean culture is what you need for LC work.







thats pretty cool man =D


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