Home | Community | Message Board


Edabea
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: Agar, Parafilm, Petri Dish

Jump to first unread post. Pages: 1
Offlinedaywalker
Do you dream in color?

Registered: 10/21/03
Posts: 783
Loc: in my skin
Last seen: 7 years, 11 months
film on agar
    #2441613 - 03/17/04 01:29 AM (12 years, 8 months ago)

I have got this film on all of the agar plates. The agar is completely set, no condensation on the lid but is this just water?

When you tilt it, you can see the liquid run down. The spores have germinated so I am not too concerned, but is there any way to avoid it?


--------------------
"I suggest we ... learn to love our ... selves before it ... becomes illegal."


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 3 months, 2 days
Re: film on agar [Re: daywalker]
    #2441658 - 03/17/04 01:48 AM (12 years, 8 months ago)

If it's not contaminated then it's caused by the water that condensates on the agar surface after the agar solidifies and IME it depends from the material used for wrapping. I tested 3 types of wrapping:
micropore medical tape - no condensation, but cultures dried quickly
polyethylene wrap - lots of condensation, probably doesn't allow much air exchange
parafilm - far the best. It seems expensive at first look, but it's very strechable, so you can use 1 roll for lots of petris. I'll never try anything else.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedaywalker
Do you dream in color?

Registered: 10/21/03
Posts: 783
Loc: in my skin
Last seen: 7 years, 11 months
Re: film on agar [Re: zeronio]
    #2441663 - 03/17/04 01:52 AM (12 years, 8 months ago)

They aren't contaminated as far as I can tell. Nothing nasty. May have to try that parafilm and see how these current cultures turn out in the meantime.

thanks for the info. cheers!


--------------------
"I suggest we ... learn to love our ... selves before it ... becomes illegal."


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 15 days
Re: film on agar [Re: daywalker]
    #2441729 - 03/17/04 02:14 AM (12 years, 8 months ago)

Try this method next time to prevent condensation at pouring:
www.fungifun.org/agar


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: film on agar [Re: Anno]
    #2446890 - 03/18/04 01:30 PM (12 years, 8 months ago)

I found Anno's link very helpful. Another suggestion: Flip the petri dishes so that the side with the condensation is at the bottom (agar on top). I found that this helps with the condensation within a day or two.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedaywalker
Do you dream in color?

Registered: 10/21/03
Posts: 783
Loc: in my skin
Last seen: 7 years, 11 months
Re: film on agar [Re: daywalker]
    #2449281 - 03/18/04 11:12 PM (12 years, 8 months ago)

Thanks. I tried doing it that way and my water in the mugs must not have been hot enough. I think I got it figured out now. Latest plates look really good.

cheers!


--------------------
"I suggest we ... learn to love our ... selves before it ... becomes illegal."


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: Agar, Parafilm, Petri Dish

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Agar does not solidify when PH is dropped ! ragadinks 2,998 11 02/04/04 01:41 PM
by ragadinks
* Lower the PH of agar ?
( 1 2 all )
ragadinks 2,878 20 01/19/04 01:01 PM
by Yarry
* Post deleted by MOE THE MAD SCIENTIST phreedom 684 9 12/05/02 01:37 PM
by EvilGir
* Few Noob Questions About Using Petri Dishes And Agar glenbelt 4,520 14 02/03/09 12:13 AM
by The Shape
* couple questions about agar CLuB99 1,207 5 08/26/01 02:02 PM
by Anno
* Re: I found out I'm getting an agar set for xmas! Anonymous 1,317 10 12/22/99 07:22 PM
by AnubisRonin
* Agar Strain Isolation - Is it worth it?
( 1 2 all )
Blue Helix 5,001 20 02/23/05 03:07 AM
by Blue Helix
* Salicylic Acid Agar
( 1 2 all )
P.Menace 4,169 38 08/10/08 06:16 PM
by P.Menace

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Prisoner#1, RogerRabbit, EvilMushroom666
732 topic views. 0 members, 0 guests and 0 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.001 seconds on 14 queries.