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Offlinefunky_cracker
backinthepile

Registered: 12/11/03
Posts: 58
Last seen: 14 years, 7 months
WHats goin on here????
    #2408482 - 03/08/04 06:34 PM (20 years, 25 days ago)

Hey, hows it goin?
My FOAF made his first print/syringe a few weeks ago and the jars o' grain he inoculated around two weeks ago haven't shown any growths at all. He finds this odd because he usually sees the first mycelium in two or three days. The only thing he noticed different between his and the syringes hes got from other people is that the spores were more like flakes and the syrines he bought were more like blobs or clouds. By the way he used this tek: www.shroomery.org/index/par/7958
He followed it exactley except he used a small country crock tub instead of the yogurt container.
Whats the deal????? Should he try a different syringe/sporemaking tek? What works the best?
thanks and mcpeace

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InvisibleCoolBlue
n00b
Registered: 09/16/03
Posts: 619
Re: WHats goin on here???? [Re: funky_cracker]
    #2408570 - 03/08/04 07:00 PM (20 years, 25 days ago)

shot some of the spores into a karo or malt solution and see if anything grows thats the best way to check your syringes

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Offlinefunky_cracker
backinthepile

Registered: 12/11/03
Posts: 58
Last seen: 14 years, 7 months
Re: WHats goin on here???? [Re: funky_cracker]
    #2412891 - 03/09/04 10:20 PM (20 years, 24 days ago)

Can anyone say why it didn't work? Or a better way to make syringes?

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OfflineScourge
The Dr.
Registered: 12/03/00
Posts: 293
Loc: Near flint Michgan, USA
Last seen: 18 years, 3 months
Re: WHats goin on here???? [Re: funky_cracker]
    #2413125 - 03/09/04 11:25 PM (20 years, 24 days ago)

perhaps bacterial infection or incompleat sterilization. a bactera could infect all the substrate and leave behind little evidance to the untrained eye. It's likly that there would be no growth on such a substrate. Are you using filter patches? if so put your nose up to it and give it a wiff. if it's bacteria that should tell you. if you dont use patches, you may want to sacrifice a jar to give the sniff test, sence they are doing nothing anyhow.


--------------------
"Those who walk in Love and Truth shall grow in Honor and Strength."

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Offlinefunky_cracker
backinthepile

Registered: 12/11/03
Posts: 58
Last seen: 14 years, 7 months
Re: WHats goin on here???? [Re: Scourge]
    #2435486 - 03/15/04 03:11 PM (20 years, 18 days ago)

Well, he sniffed the jar and it smelled like his pressure cooker after he sterilizes jars of grain, no funny smells at all. He did the jars the way he has for a while and it never fails. So, should he try a different syringe tek? Which one? Someone please help him.

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Offline003
mr. getSome

Registered: 10/20/03
Posts: 147
Loc: Kanata
Last seen: 11 years, 29 days
Re: WHats goin on here???? [Re: funky_cracker]
    #2435508 - 03/15/04 03:16 PM (20 years, 18 days ago)

I recently made my first prints also and had the same problem with a couple syringes. How long did the spores sit in the solution before you inoculated? If you innoculated soon after the syringes were made, they might not have had time to rehydrate resulting in slow or no growth.

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Offlinefunky_cracker
backinthepile

Registered: 12/11/03
Posts: 58
Last seen: 14 years, 7 months
Re: WHats goin on here???? [Re: 003]
    #2441486 - 03/16/04 10:43 PM (20 years, 17 days ago)

He made two syringes. One was used after about 12 hours after it was made, and the other was used a week after it was made.

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Invisiblegeorge castanzaM
Lord Of The Idiots!
Male User Gallery
Registered: 10/21/02
Posts: 8,749
Trusted Cultivator
Re: WHats goin on here???? [Re: funky_cracker]
    #2441518 - 03/16/04 10:54 PM (20 years, 17 days ago)

mabye syringe water was too hot?


--------------------
KRAMER CAKES



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Offlinefunky_cracker
backinthepile

Registered: 12/11/03
Posts: 58
Last seen: 14 years, 7 months
Re: WHats goin on here???? [Re: george castanza]
    #2441553 - 03/16/04 11:05 PM (20 years, 17 days ago)

Nope.
:stoned:

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Invisiblegeorge castanzaM
Lord Of The Idiots!
Male User Gallery
Registered: 10/21/02
Posts: 8,749
Trusted Cultivator
Re: WHats goin on here???? [Re: funky_cracker]
    #2441574 - 03/16/04 11:11 PM (20 years, 17 days ago)

?????????????????    try again :frown:


--------------------
KRAMER CAKES



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Offlinefunky_cracker
backinthepile

Registered: 12/11/03
Posts: 58
Last seen: 14 years, 7 months
Re: WHats goin on here???? [Re: george castanza]
    #2441587 - 03/16/04 11:17 PM (20 years, 17 days ago)

Should I try a different tek? Which one?

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Invisiblegeorge castanzaM
Lord Of The Idiots!
Male User Gallery
Registered: 10/21/02
Posts: 8,749
Trusted Cultivator
Re: WHats goin on here???? [Re: funky_cracker]
    #2441594 - 03/16/04 11:20 PM (20 years, 17 days ago)

kramer bakes a 1/2 pint @450F for an hour in place of the yougurt cup. he claims it works :crazy2:


--------------------
KRAMER CAKES



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