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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather] * 1
    #24333528 - 05/19/17 04:58 PM (6 years, 8 months ago)

Sorted, here is the setup, Tarragon spawn at the bottom.

   


Edited by Ferather (05/20/17 12:54 PM)


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24337543 - 05/21/17 08:34 AM (6 years, 8 months ago)

WL-Tek + sugar germinates spores in 3-4 days.


Substrate, non sterile:

Optional: 25-50g > Dry Compost Powder (Sieved), Calcium Water (pH 8-10).

3g, (3.75ml) > Miracle-Gro Nutrients.
0.5g, (0.62ml) > Yeast Nutrients.
100g > Dry Pellets.


Spore work, aspectic:

Optional: Calcium Water (pH 8).

3g, (3.75ml) > Miracle-Gro Nutrients.
0.5g, (0.62ml) > Yeast Nutrients.
6g, (5.68ml) > Sucrose.
100g > Dry Pellets.


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24337592 - 05/21/17 09:10 AM (6 years, 8 months ago)

Here is the Tarragon oyster, thick aggressive growth.

   


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24337667 - 05/21/17 09:47 AM (6 years, 8 months ago)

For a semi aseptic easy way to germinate your spores, make sugar free WL-Tek (enrichment), add your spores, use a toothpick marker.
Assemble a sterile sucrose + water, 6g sucrose to 100g water, feeding syringe, and add a few drops to your spores.

Any infections will be localized to areas with added sugar, transfers (outer edge) can be used.


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24337672 - 05/21/17 09:52 AM (6 years, 8 months ago)

If you are using wood pellets, of any kind, buy some pH strips, and increase the pH to 6.5-8 (depends on the mycelium).
Use only calcium products, ideally free of any magnesium or sodium, which become toxic at certain levels.

Mycelium appear to have no known toxicity level with CaCO3, only reactions to the pH.


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather] * 1
    #24379536 - 06/05/17 12:38 PM (6 years, 7 months ago)

Not that a normal microwave will go above 120-150°C, but here is an interesting image for microwaving cellulose.



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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24385069 - 06/07/17 11:42 AM (6 years, 7 months ago)

I got some store bought black poplar, which smelled like earthy potato peel.
After 4 days, the whole substrate smells like earthy potato peel.

I'm guessing this is normal for black poplar clones?


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24391099 - 06/09/17 12:59 PM (6 years, 7 months ago)

Compilation of data, for better awareness of materials:


Manure, hydrated, note the three main nutrients, contains both ammoniacal and ureic nitrogen.



----

Wood ash, notice P2O5 and K2O, as well as calcium and other, has a high pH.



----

Soluble nutrients, here are the items I am using, note the nutrients.

     

----

Effect of nitrogen sources on the growth of oyster mushroom PO and PC

----

Proteins and some vitamins, and other (such as caffeine, C8-H10-N4-O2), can-are utilized as nitrogen sources.

Composition of: Tea leaves, Coffee grounds, Wheat bran, Pleurotus ostreatus.

----

Ideally you want the substrate pH at the highest tolerance for your mycelium, or slightly lower, to reduce contamination chances.




Rice pH (all cooked) - not modified. -- Source

Brown: 6.20 - 6.80
Krispies: 5.40 - 5.73
White: 6.00 - 6.70
Wild: 6.00 - 6.50

----





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Edited by Ferather (11/09/17 05:55 AM)


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24391131 - 06/09/17 01:12 PM (6 years, 7 months ago)

Organic nutrients may contain bacteria, handle as necessary.


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24391242 - 06/09/17 02:06 PM (6 years, 7 months ago)

Here is a very basic list for cultivating mycelium, adapt as necessary.


Key: [X] means this value can change depending on the species and-or strain.


Building a colony:  [X] spawn >> [X] substrate >> [X] pH >> [X] enrichment (optional) >> [X] water content >> Container with [X] air exchange and [X] humidity >> 22-24°C (71.6-75.2°F), [X], colonization and consolidation, [X] time span.
Fruiting a colony:  Consolidated colony >> [X] triggers and [X] temperature to produce primordia formation (there are many) >> [X] temperature, [X] humidity and [X] air exchange to fully produce fruit body's.


Tips:

> In cases of secondary and tertiary decomposers, take the necessary steps to grow-fruit them correctly.
> Some mycelium may prefer pasteurized substrates, some may prefer fully sterilized substrates.


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24391243 - 06/09/17 02:06 PM (6 years, 7 months ago)

If you wish to cultivate fruits outdoors and know the optimal settings (you may need to measure your fruiting area), you can wait until nature provides them.
Methods: Isolated tubs or large plant pots, beds with stone-pea shingle or other barriers, a plastic pond, membrane or plastic sheet.

Pretty much anything that holds your mycelium in place and helps prevent critters, while allowing drainage.


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24441586 - 06/28/17 08:08 AM (6 years, 6 months ago)

Got new agar, it also has other names.



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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24441591 - 06/28/17 08:12 AM (6 years, 6 months ago)

100g agar = 50 cultures @ 2g agar + 100g water, or 5 liters total.


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24441619 - 06/28/17 08:39 AM (6 years, 6 months ago)

End pH data for oyster substrates, external links:


"Coconut fiber recorded the shortest duration to pinning and a 5 times increase in ABE when pH was adjusted to 6.0. Among the substrates tested, sawdust recorded more than 10 times increase in ABE at pH 7.0..." -- Source

"In conclusion use of 2% (pH 7.8) lime is good for the production of Oyster mushroom (Pleurotus spp.) using cotton waste as a substrate." -- Source

----

Here is pine (about pH 4.4, see above), modified to pH 7.5 (CaCO3):

   


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OfflineFerather
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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24441622 - 06/28/17 08:40 AM (6 years, 6 months ago)

Conclusion: End pH (varies) effects mycelial BE on substrates.


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24498040 - 07/21/17 06:26 AM (6 years, 6 months ago)

Carbonater: Genetics, coming to a cinema near you!


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24502221 - 07/23/17 06:56 AM (6 years, 6 months ago)

Mycelium which do not require symbiosis or alternative organisms in order to grow and fruit (even 1 fruit) are primary decomposers.
Types of mycelium still fitting this category, that are fussy, restricted, or media specific, are still primary decomposers.

Button mushrooms for example, target very few proteins, and only detects them in small units.
It is therefore restricted in genetics (detection-digestion), but not fully.


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24522431 - 08/01/17 09:33 AM (6 years, 5 months ago)

For those interested in wood, paper pellets or cardboard, and visibly looking for contamination.


> No starch or sugar added, essentially all cellulose, or cellulose and lignin (+other).
> Wood loving molds, usually fruitless and coloured, else substrate browning.

- Due to a cellulose or an adaptable micro organism, wood compatible.



> Starch and-or sugar added, essentially universal, mixed carbon sources.
> Almost anything that germinates, mold, yeast, bacteria, other.

- No browning, dots, fruiting in unusual areas (not surface) likely yeast.
- Browning, slime, smells, likely due to bacteria or other.



You can also get random fruiting mycelium in both cases.


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24522443 - 08/01/17 09:43 AM (6 years, 5 months ago)

In order to prevent mold and other micro-organisms you will need to alter the end pH of the substrate.
Within range for your live mycelium, but high enough to help stop spore germination.

Next you need a complex carbon medium with no "soluble" or "easy" carbon.
The complex medium should be enriched, and grain spawn used.

The starchy grain will act as the "easy" carbon.


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Re: Ferather's Corner | Experiments, tests and data [Re: Ferather]
    #24522562 - 08/01/17 10:45 AM (6 years, 5 months ago)

You the man, Ferather!


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