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Offlinedoc34
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For those of you who said it couldnt be done!
    #2438219 - 03/16/04 08:19 AM (12 years, 8 months ago)

A friend of mine wanted to try and see if he could take a "wild print"and use it to colonate pf jars.Out of twelve jars he managed to get one that has prominent myc growth.
Now hold on before you say "Contams are on their way"!Under the controled invironment of a glovebox he took the healthiest part of the myc and made a honey syringe and it worked just fine-now then ,he innoculated 12 more jars from this and now he has - so far 10 jars showing growth- he is very wary of contams and as soon as he sees any hint of contams he then takes the healthest part of myc and transfers it to a syringe(honey) and he innocs more jars.
So far he has done well-IMO.
I thought I would post this cause if you use your head you can do this without agar-you may not be able to isolate a strain but you can grow useing a wild print-just a lot of work.But for those of us who cant find agar , I'm sure this could be an option.
As soon as he can he'll post pics of his progress. :thumbup:


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InvisibleMilkVein
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Re: For those of you who said it couldnt be done! [Re: doc34]
    #2438325 - 03/16/04 09:12 AM (12 years, 8 months ago)

had a friend on the board send my a wild print...made one jar...1/2pint...3 flushs..maybe like 10g in all...it didnt go to contams...and thanks for that print ->bullet.


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Offlinedoc34
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Re: For those of you who said it couldnt be done! [Re: MilkVein]
    #2438390 - 03/16/04 09:33 AM (12 years, 8 months ago)

Could this be a "Poor man's non-agar tek"?


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Edited by doc34 (03/16/04 09:34 AM)


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InvisibleSemilanceata
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Re: For those of you who said it couldnt be done! [Re: doc34]
    #2438521 - 03/16/04 10:29 AM (12 years, 8 months ago)

Good for you man, but was it good luck or do you think this procedure is going to work more than one time?. IMO, that?s the important thing.


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OfflineEkstaza
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Re: For those of you who said it couldnt be done! [Re: doc34]
    #2438531 - 03/16/04 10:32 AM (12 years, 8 months ago)

Being from Georgia, I had to use wild prints out of neccessity and my first grow went ok. You just have to pay more attention to your sterile techniques is all.


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Offlinedoc34
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Re: For those of you who said it couldnt be done! [Re: Semilanceata]
    #2438620 - 03/16/04 11:26 AM (12 years, 8 months ago)

Well,I'm sure luck is playing a factor,but 10 out of twelve jars look great and that is pretty good odds I believe!I think it will work out as long as he can keep contams at a minnimum .
If you take a jar that gets contams and you transfer some healthy myc to a honey or karo jar, even to another jar, you have just decreased your contamination rate by say at least half maybe more(maybe more sounds more like it)and then if you transfer from this one to another one you have decreased your contam rate from your original jar to say 20%(dont hold me to my figures this is just a guesstimate)this is basically the same thing as "agar" but its more "barbaric" lol.I think he is on to something cheap and effecient :wink:


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Offlinedoc34
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Re: For those of you who said it couldnt be done! [Re: Ekstaza]
    #2438633 - 03/16/04 11:30 AM (12 years, 8 months ago)

Quote:

Ekstaza said:
Being from Georgia, I had to use wild prints out of neccessity and my first grow went ok. You just have to pay more attention to your sterile techniques is all.




Exactly!Plus being creative adds "spice"to it.IMO,I think things like this is how we find new facts and new teks and new- well you know what I mean. :wink:

I am not saying that this can replace agar or anything of the sort- I'm not a grower-therefore I dont know, but,this seems to be something that any noobie can do instead of forking out $20.00 for a print or syringe ,or having to purchase agar and petri dishes and an autoclave(pressure cooker) when you can do the same thing (basically) with one "wild mushroom"and the standard pf tek "improvised" all under $20.00!
Maybe I should have posted this in basic cultivation(just realised)sorry-but then again its something to look at by all. :smirk:


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Edited by doc34 (03/16/04 12:37 PM)


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